FastQCFastQC Report
Thu 26 May 2016
SRR1545215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545215_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences150266
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.387313164654679No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3290.2189450707412189No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2040.1357592535902999No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1960.13043536129264105No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT1700.11313271132524988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGC453.8016879E-1045.0000044
AACGGGA453.8016879E-1045.0000044
TAGGGTA453.8016879E-1045.0000045
GAACGGG302.1528795E-645.0000043
TGATCCT502.1827873E-1145.018
GTGTTAG207.0137036E-445.01
ATGGGTA253.8745828E-545.05
CATATGC502.1827873E-1145.033
TTAGCGG253.8745828E-545.02
GACGTTC207.0137036E-445.012
ACCTGGT502.1827873E-1145.011
AGGGTGC253.8745828E-545.06
CGACAAT253.8745828E-545.020
GCTTGAG253.8745828E-545.01
GTAATTT253.8745828E-545.09
CTACGGG253.8745828E-545.03
CGGGACT253.8745828E-545.06
CTACCTT207.0137036E-445.017
AATTCGC207.0137036E-445.013
GCGATGA406.7466317E-945.09