##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545215_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	150266
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203964968788682	31.0	31.0	33.0	30.0	34.0
2	31.47463830806703	31.0	31.0	34.0	30.0	34.0
3	31.56955665286891	31.0	31.0	34.0	30.0	34.0
4	35.35220209495162	37.0	35.0	37.0	33.0	37.0
5	35.19860780216416	37.0	35.0	37.0	32.0	37.0
6	35.183647664807744	37.0	35.0	37.0	32.0	37.0
7	35.823206846525494	37.0	35.0	37.0	35.0	37.0
8	35.90323160262468	37.0	35.0	37.0	35.0	37.0
9	37.54822780935142	39.0	37.0	39.0	35.0	39.0
10	36.75431568019379	39.0	37.0	39.0	32.0	39.0
11	36.43930762780669	38.0	35.0	39.0	32.0	39.0
12	35.58059707452118	37.0	35.0	39.0	31.0	39.0
13	35.233632358617385	37.0	35.0	39.0	30.0	39.0
14	36.032888344668784	38.0	35.0	40.0	30.0	41.0
15	36.42108660641795	38.0	35.0	40.0	31.0	41.0
16	36.317337255267326	38.0	35.0	40.0	31.0	41.0
17	36.3856161739848	38.0	35.0	40.0	31.0	41.0
18	36.44689417433085	38.0	35.0	40.0	31.0	41.0
19	36.371441310742284	37.0	35.0	40.0	31.0	41.0
20	36.255506901095394	37.0	35.0	40.0	31.0	41.0
21	36.14120958833003	37.0	34.0	40.0	31.0	41.0
22	36.01613139366191	37.0	34.0	40.0	30.0	41.0
23	35.931235276110364	37.0	34.0	40.0	30.0	41.0
24	35.732095084716434	37.0	34.0	40.0	30.0	41.0
25	35.696411696591376	36.0	34.0	40.0	30.0	41.0
26	35.563154672380975	36.0	34.0	40.0	29.0	41.0
27	35.5373404496027	36.0	34.0	40.0	29.0	41.0
28	35.55083651657727	36.0	34.0	40.0	29.0	41.0
29	35.53694115768038	36.0	34.0	40.0	29.0	41.0
30	35.47090492859329	36.0	34.0	40.0	29.0	41.0
31	35.370556213647795	36.0	34.0	40.0	29.0	41.0
32	35.23123660708344	36.0	34.0	40.0	29.0	41.0
33	35.058270001197876	36.0	34.0	40.0	27.0	41.0
34	34.90882834440259	36.0	34.0	40.0	27.0	41.0
35	34.75881436918531	36.0	33.0	40.0	27.0	41.0
36	34.558948797465824	36.0	33.0	40.0	25.0	41.0
37	34.39400795921898	35.0	33.0	40.0	25.0	41.0
38	34.421226358590765	35.0	33.0	40.0	25.0	41.0
39	34.43289233758801	35.0	33.0	40.0	25.0	41.0
40	34.25353040607988	35.0	33.0	40.0	24.0	41.0
41	34.273009197023946	35.0	33.0	40.0	24.0	41.0
42	34.181744373311325	35.0	33.0	40.0	24.0	41.0
43	34.06730730837315	35.0	33.0	40.0	24.0	41.0
44	33.99472934662532	35.0	33.0	40.0	24.0	41.0
45	33.93114210799516	35.0	33.0	39.0	23.0	41.0
46	33.857040182077114	35.0	33.0	39.0	23.0	41.0
47	33.87929405188133	35.0	33.0	39.0	23.0	41.0
48	33.83825349713175	35.0	33.0	39.0	23.0	41.0
49	33.8299016410898	35.0	33.0	39.0	24.0	41.0
50	33.61974764750509	35.0	33.0	39.0	24.0	41.0
51	33.45227130555149	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	3.0
16	12.0
17	30.0
18	71.0
19	178.0
20	270.0
21	506.0
22	685.0
23	946.0
24	1323.0
25	1860.0
26	2435.0
27	2980.0
28	3282.0
29	3885.0
30	4795.0
31	5782.0
32	7244.0
33	9477.0
34	15378.0
35	21560.0
36	11220.0
37	13630.0
38	18122.0
39	24575.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.538751281061586	23.97881090865532	27.915163776236806	13.567274034046292
2	31.96331838206913	26.481705775092166	28.72372991894374	12.83124592389496
3	30.011446368439966	25.776290045652377	30.60572584616613	13.606537739741526
4	26.922923349260646	28.20598139299642	30.101952537500164	14.76914272024277
5	23.394513728987263	32.74526506328777	28.419602571439977	15.44061863628499
6	21.300227596395725	41.57028203319447	27.048700304792835	10.080790065616972
7	87.19337707798172	4.840083585109074	6.153088523019179	1.813450813890035
8	88.40988646799676	3.9855988713348327	5.9780655637336455	1.626449096934769
9	84.40698494669454	5.705881570015839	7.1712829249464285	2.7158505583432047
10	51.213847443866214	24.047356021987675	13.138700704084757	11.600095830061358
11	43.98799462286878	22.35435827133217	19.468143159463885	14.189503946335167
12	39.468675548693646	21.85723982803828	24.766746968708823	13.90733765455925
13	21.584390347783263	38.33002808353187	24.344163017582154	15.741418551102711
14	15.808632691360655	40.71979023864347	27.382774546470923	16.08880252352495
15	15.684186708902878	23.834400330081323	45.222472149388416	15.258940811627381
16	17.759173731915403	19.319074175129437	43.393715145142615	19.528036947812545
17	16.939294318075945	20.146273940878174	29.94157028203319	32.972861459012684
18	21.473254096069635	23.45041459811268	34.86417419775598	20.212157108061703
19	31.16473453742031	23.521621657593865	25.593281247920352	19.72036255706547
20	31.959990949383094	22.542690961361853	25.85282099743122	19.64449709182383
21	23.489678303807914	28.724395405480944	27.519199286598433	20.266727004112706
22	22.86012803960976	25.361026446434987	23.745225134095538	28.033620379859713
23	19.959937710460117	30.865265595677	25.760983855296608	23.413812838566276
24	19.731010341660788	23.820425112799967	38.97288807847417	17.47567646706507
25	18.97634860846765	25.51941224229034	34.98196531484168	20.52227383440033
26	17.691294105120253	35.3732713987196	25.930017435747278	21.005417060412867
27	18.678210639798756	35.52367135612847	27.213740966020257	18.584377038052523
28	16.821503200990243	28.800926357259794	36.25836849320538	18.119201948544582
29	17.25739688286106	25.929351949210066	34.83622376319327	21.9770274047356
30	19.547336057391558	32.72796241332038	28.724395405480944	19.000306123807114
31	29.584869498090054	27.69621870549559	24.298244446514847	18.42066734989951
32	31.570681325116794	25.58196797678783	25.862137808952124	16.985212889143252
33	30.268324171802004	26.446434988620183	23.496333169179987	19.78890767039783
34	20.991441843131515	26.581528755673272	26.69532695353573	25.731702447659483
35	22.10546630641662	25.019631852847617	30.995700956969642	21.87920088376612
36	30.85794524376772	27.32421173119668	23.745225134095538	18.072617890940066
37	20.853686129929592	35.24549798357579	26.487029667389827	17.413786219104786
38	20.774493232001916	35.536315600335406	23.329961534878148	20.35922963278453
39	22.41757949236687	33.79274087285214	24.211065710140684	19.578613924640305
40	25.979928926037825	25.90073602811015	26.101047475809562	22.018287570042457
41	18.08126921592376	25.46351137316492	27.18845247760638	29.26676693330494
42	20.789799422357685	26.454420827066667	24.014081695127306	28.74169805544834
43	21.42267711924188	25.552021082613496	25.83219091477779	27.193110883366828
44	18.704164614749843	30.245697629536956	29.89897914365192	21.151158612061277
45	18.45860008252033	37.97399278612594	23.101699652616027	20.465707478737706
46	20.76584190701822	34.98263080137889	24.77539829369252	19.476128997910372
47	21.362783330893215	28.22594598911264	26.13764923535597	24.273621444638174
48	21.911144237552076	25.40561404442788	32.36859968322841	20.314642034791635
49	21.86389469341035	26.58884910758255	31.539403457868048	20.007852741139047
50	19.02426363914658	35.347317423768516	25.961295302996017	19.66712363408888
51	18.719470805105612	34.00902399744453	25.608587438276125	21.662917759173734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	384.0
1	370.0
2	356.0
3	736.0
4	1116.0
5	846.0
6	576.0
7	573.5
8	571.0
9	584.0
10	597.0
11	612.5
12	628.0
13	626.0
14	624.0
15	604.5
16	585.0
17	554.0
18	523.0
19	528.0
20	533.0
21	528.5
22	524.0
23	577.5
24	631.0
25	780.5
26	1203.5
27	1477.0
28	1906.5
29	2336.0
30	2505.5
31	2675.0
32	3204.0
33	3733.0
34	4037.5
35	4342.0
36	4595.0
37	4848.0
38	5839.5
39	6831.0
40	8870.0
41	10909.0
42	12620.0
43	14331.0
44	14507.5
45	14684.0
46	13305.5
47	11927.0
48	11133.5
49	10340.0
50	9690.5
51	9041.0
52	8302.5
53	7564.0
54	6893.5
55	6223.0
56	5952.5
57	5682.0
58	5752.5
59	5823.0
60	5521.0
61	5219.0
62	4586.0
63	3953.0
64	3419.5
65	2886.0
66	2497.5
67	2109.0
68	1841.5
69	1574.0
70	1364.5
71	1155.0
72	1048.5
73	942.0
74	723.5
75	404.0
76	303.0
77	231.5
78	160.0
79	112.0
80	64.0
81	47.0
82	30.0
83	21.5
84	13.0
85	9.0
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	150266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.952257995820744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.74694184377776	25.60991841135054
2	9.984282102097998	7.778206646879533
3	5.9847604729037105	6.993598019512065
4	4.211371557438666	6.561697256864493
5	2.9556481924417413	5.756458546843597
6	2.2773867286270755	5.322561324584404
7	1.6059591334654548	4.378901414824378
8	1.260848766486708	3.929032515672208
9	0.9909109546914507	3.473839724222379
>10	4.951137839130732	28.621244992213807
>50	0.020501605959133466	0.5057697682775877
>100	0.008542335816305612	0.6814582141003288
>500	0.0017084671632611223	0.387313164654679
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.387313164654679	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	329	0.2189450707412189	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	204	0.1357592535902999	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	196	0.13043536129264105	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	170	0.11313271132524988	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014640703818561751	0.0
2	0.0	0.0	0.0	0.04591857106730731	0.0
3	0.0	0.0	0.0	0.06721414025794258	0.0
4	0.0	0.0	0.0	0.09117165559740727	0.0
5	0.0	0.0	0.0	0.17502295928553366	0.0
6	0.0	0.0	0.0	0.26486364180852623	0.0
7	0.0	0.0	0.0	0.32675388976881	0.0
8	0.0	0.0	0.0	0.493125524070648	0.0
9	0.0	0.0	0.0	0.5530193124193098	0.0
10	0.0	0.0	0.0	0.6628245910585229	0.0
11	0.0	0.0	0.0	0.7866050869790904	0.0
12	0.0	0.0	0.0	0.8811041752625345	0.0
13	0.0	0.0	0.0	0.9290192059414638	0.0
14	0.0	0.0	0.0	0.946321855908855	0.0
15	0.0	0.0	0.0	0.982258128918052	0.0
16	0.0	0.0	0.0	1.0308386461341887	0.0
17	0.0	0.0	0.0	1.0874050017968135	0.0
18	0.0	0.0	0.0	1.173252765096562	0.0
19	0.0	0.0	0.0	1.21650939001504	0.0
20	0.0	0.0	0.0	1.2557730957102737	0.0
21	0.0	0.0	0.0	1.3096775052240692	0.0
22	6.654865372073522E-4	0.0	0.0	1.3609199685890354	0.0
23	6.654865372073522E-4	0.0	0.0	1.4401128665167104	0.0
24	6.654865372073522E-4	0.0	0.0	1.5086579798490676	0.0
25	6.654865372073522E-4	0.0	0.0	1.5579039836024118	0.0
26	6.654865372073522E-4	0.0	0.0	1.6151358258022441	0.0
27	6.654865372073522E-4	0.0	0.0	1.655065018034685	0.0
28	6.654865372073522E-4	0.0	0.0	1.7089694275484808	0.0
29	6.654865372073522E-4	0.0	0.0	1.7681977293599351	0.0
30	6.654865372073522E-4	0.0	0.0	1.856707438808513	0.0
31	6.654865372073522E-4	0.0	0.0	1.9325729040501511	0.0
32	6.654865372073522E-4	0.0	0.0	1.9991215577708863	0.0
33	6.654865372073522E-4	0.0	0.0	2.067001184566036	0.0
34	6.654865372073522E-4	0.0	0.0	2.14153567673326	0.0
35	6.654865372073522E-4	0.0	0.0	2.238696711165533	0.0
36	6.654865372073522E-4	0.0	0.0	2.317224122556001	0.0
37	6.654865372073522E-4	0.0	0.0	2.3977479935580903	0.0
38	6.654865372073522E-4	0.0	0.0	2.4815992972462166	0.0
39	6.654865372073522E-4	0.0	0.0	2.558130249025062	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGGC	45	3.8016879E-10	45.000004	4
AACGGGA	45	3.8016879E-10	45.000004	4
TAGGGTA	45	3.8016879E-10	45.000004	5
GAACGGG	30	2.1528795E-6	45.000004	3
TGATCCT	50	2.1827873E-11	45.0	18
GTGTTAG	20	7.0137036E-4	45.0	1
ATGGGTA	25	3.8745828E-5	45.0	5
CATATGC	50	2.1827873E-11	45.0	33
TTAGCGG	25	3.8745828E-5	45.0	2
GACGTTC	20	7.0137036E-4	45.0	12
ACCTGGT	50	2.1827873E-11	45.0	11
AGGGTGC	25	3.8745828E-5	45.0	6
CGACAAT	25	3.8745828E-5	45.0	20
GCTTGAG	25	3.8745828E-5	45.0	1
GTAATTT	25	3.8745828E-5	45.0	9
CTACGGG	25	3.8745828E-5	45.0	3
CGGGACT	25	3.8745828E-5	45.0	6
CTACCTT	20	7.0137036E-4	45.0	17
AATTCGC	20	7.0137036E-4	45.0	13
GCGATGA	40	6.7466317E-9	45.0	9
>>END_MODULE