FastQCFastQC Report
Thu 26 May 2016
SRR1545214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545214_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences55288
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.41057734047171174No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.2731153233974823No Hit
TGTAGCGGGGTTAAAGACAGCGAGAAAAATTTTTGCTGGAAAAACACGGAG1190.21523657936622775No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG830.15012299233106643No Hit
ACACCAGGGTCCAGAAAGCTGTGCAGTCCTAGGAGAATTAACACTGCAATC800.1446968600781363No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT620.11214006656055564No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC580.10490522355664882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACA453.7107384E-1045.00000445
AGGGCGA453.7107384E-1045.0000046
TGATACG351.18408934E-745.00000412
ATACGGC351.18408934E-745.00000414
TGGGCGA351.18408934E-745.0000046
TTCATTA253.8426377E-545.016
ATGGGCG206.975075E-445.05
GTTGAGG206.975075E-445.02
CAATGGG206.975075E-445.03
CACATCC206.975075E-445.045
TCTAGGG206.975075E-445.03
GCGATGT206.975075E-445.09
CAACCTC206.975075E-445.027
TCAGGGA253.8426377E-545.04
TATGTTC253.8426377E-545.012
AGGATAG206.975075E-445.01
TAAGCAG206.975075E-445.01
AGCTAGG253.8426377E-545.02
GGCGATA206.975075E-445.08
GTTCATT253.8426377E-545.015