FastQCFastQC Report
Thu 26 May 2016
SRR1545213_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545213_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1624966
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56200.34585339016324035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54070.33274542359655523No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37700.23200485425541212No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT23710.14591074520943823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG600.045.0000041
ACACGTG207.0341484E-445.00000414
ACCGGAT207.0341484E-445.00000415
ACACCGC207.0341484E-445.00000431
TGGAACG650.045.0000041
TTGTACG406.8175723E-945.0000041
CACGACA600.045.00000414
TCTAGCG406.8175723E-945.0000041
TGCGCAT207.0341484E-445.0000041
TACGATG302.166058E-645.0000041
CGTACTG207.0341484E-445.00000411
AGACACG207.0341484E-445.00000422
CATACGT207.0341484E-445.00000421
ACGCTAT406.8175723E-945.00000435
TCCGTCG302.166058E-645.0000042
CAAGCCG207.0341484E-445.00000445
GTTCACG750.045.0000041
TTAGTCA207.0341484E-445.00000444
CGTAAGA207.0341484E-445.00000422
AACCTCG302.166058E-645.00000422