##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545213_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1624966 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.257103225544412 31.0 31.0 34.0 30.0 34.0 2 31.508739259775282 31.0 31.0 34.0 30.0 34.0 3 31.59775342991792 31.0 31.0 34.0 30.0 34.0 4 35.33598549138874 37.0 35.0 37.0 33.0 37.0 5 35.1788628192836 37.0 35.0 37.0 32.0 37.0 6 35.167792433810924 37.0 35.0 37.0 32.0 37.0 7 35.84756173359935 37.0 35.0 37.0 35.0 37.0 8 35.9765035083811 37.0 35.0 37.0 35.0 37.0 9 37.66988970846159 39.0 37.0 39.0 35.0 39.0 10 36.88503759463275 39.0 37.0 39.0 32.0 39.0 11 36.408906401733944 38.0 35.0 39.0 32.0 39.0 12 34.92291346403555 35.0 34.0 39.0 30.0 39.0 13 34.14409963039227 35.0 33.0 39.0 25.0 39.0 14 34.81431426872931 35.0 33.0 40.0 25.0 41.0 15 35.50242528151358 35.0 33.0 40.0 30.0 41.0 16 35.61701783298851 35.0 34.0 40.0 31.0 41.0 17 35.7246908550702 35.0 34.0 40.0 31.0 41.0 18 35.8604075408347 36.0 35.0 40.0 31.0 41.0 19 35.77024073119068 36.0 35.0 40.0 31.0 41.0 20 35.56418226596741 35.0 34.0 40.0 30.0 41.0 21 35.37529954472894 35.0 34.0 40.0 30.0 41.0 22 35.249027364264855 35.0 34.0 40.0 30.0 41.0 23 35.2739651168086 35.0 34.0 40.0 30.0 41.0 24 35.104235411694766 35.0 34.0 40.0 29.0 41.0 25 35.09658786706922 35.0 34.0 39.0 30.0 41.0 26 34.96076410214121 35.0 34.0 39.0 29.0 41.0 27 34.864585474403775 35.0 34.0 39.0 29.0 41.0 28 35.00954666128399 35.0 34.0 39.0 29.0 41.0 29 35.09401550555519 36.0 34.0 39.0 29.0 41.0 30 35.04605450206343 36.0 34.0 39.0 29.0 41.0 31 34.822769830261066 35.0 34.0 39.0 29.0 41.0 32 34.65441492314301 35.0 34.0 39.0 29.0 41.0 33 34.45114974713317 35.0 33.0 39.0 27.0 41.0 34 34.3556880574732 35.0 34.0 39.0 27.0 41.0 35 34.18425185511574 35.0 33.0 39.0 27.0 41.0 36 33.89185189105495 35.0 33.0 39.0 24.0 41.0 37 33.68869194801614 35.0 33.0 39.0 23.0 41.0 38 33.84664417593968 35.0 33.0 39.0 25.0 41.0 39 33.90551371536389 35.0 33.0 39.0 24.0 41.0 40 33.689885203751956 35.0 33.0 39.0 23.0 41.0 41 33.81461273651264 35.0 33.0 39.0 24.0 41.0 42 33.79169902631809 35.0 33.0 39.0 24.0 41.0 43 33.64077894552871 35.0 33.0 39.0 24.0 41.0 44 33.62512754113009 35.0 33.0 38.0 24.0 41.0 45 33.576122208095434 35.0 33.0 38.0 24.0 41.0 46 33.54997273789113 35.0 33.0 38.0 23.0 40.0 47 33.58280850183942 35.0 33.0 38.0 24.0 40.0 48 33.62289180204386 35.0 33.0 38.0 24.0 40.0 49 33.680426544309235 35.0 33.0 38.0 24.0 40.0 50 33.41048735788934 35.0 33.0 38.0 24.0 40.0 51 33.20186699290939 35.0 32.0 38.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 3.0 13 11.0 14 14.0 15 56.0 16 166.0 17 434.0 18 1000.0 19 2004.0 20 3433.0 21 5432.0 22 8169.0 23 11784.0 24 16267.0 25 22491.0 26 28731.0 27 33491.0 28 38181.0 29 44303.0 30 54160.0 31 66596.0 32 84815.0 33 113803.0 34 205477.0 35 315969.0 36 93682.0 37 105926.0 38 145601.0 39 222744.0 40 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.43382692314793 25.69186062969933 29.54597203879958 13.32834040835316 2 28.03166343172719 26.681973653602597 31.90503678230806 13.381326132362153 3 27.08315127824213 26.597664197281667 33.203094710904715 13.116089813571483 4 24.64550027508268 28.29468431954884 31.65561617904621 15.404199226322273 5 21.76279380614733 33.164140049699505 30.867845850313174 14.205220293839993 6 20.908191309848945 41.32664929604681 27.056935345108755 10.708224048995486 7 87.7072504901641 5.619379113162983 5.361527564269037 1.311842832403878 8 91.13704532894842 3.3017306208252974 4.492463226922902 1.0687608233033798 9 87.72208156970669 4.412523092790865 5.977971231398072 1.887424106104374 10 63.222184341087754 20.169652780427406 9.25237820360549 7.3557846748793505 11 58.83163093873964 14.74584699002933 17.763263969830753 8.659258101400276 12 53.25988359141053 20.074573868007086 17.615568571896272 9.049973968686114 13 17.858958279742467 55.105399128350996 17.499996923012542 9.535645668893995 14 10.817457103717862 54.095962623217964 23.99914828987191 11.087431983192264 15 8.451192209560077 21.533865939348885 59.473921300507214 10.541020550583829 16 10.18310536958927 15.1878870080974 55.72775061139741 18.90125701091592 17 10.659607647175386 15.893932549973352 28.621275768231456 44.8251840346198 18 17.768802547253294 20.88246769470869 41.70776496246691 19.640964795571108 19 34.27610177689871 21.287953101787977 24.546421278968296 19.889523842345007 20 35.33218541187939 21.649806826727453 24.882674468265797 18.135333293127363 21 18.227704456585553 33.71332077101921 28.08212602602147 19.976848746373772 22 19.71468941504007 25.20052727257063 20.712556447334897 34.37222686505441 23 14.963266923738713 35.524127889445076 23.30257986936342 26.210025317452796 24 14.388731825773585 21.244690658143 50.806047634227426 13.56052988185599 25 11.218634728357394 26.048175777216258 44.25526441784013 18.477925076586217 26 11.427623716434681 42.50070463012764 26.760806072250126 19.310865581187546 27 11.538395264885542 46.14527319340836 26.47710782871765 15.839223712988456 28 9.488383141554962 32.38135444064676 43.64275929465602 14.48750312314227 29 9.685310338800935 24.36334052527868 42.986991727826926 22.964357408093463 30 15.30641256493982 39.39134726511201 29.803023570954718 15.499216598993456 31 34.1109906299578 28.563797642535288 22.98700403577675 14.338207691730165 32 37.52933907540219 23.72301943548357 24.787534016096338 13.960107473017896 33 34.67555628856235 26.750221235398158 20.632370154206303 17.941852321833196 34 17.621722546810208 25.47007137380105 25.61985912320627 31.288346956182465 35 17.741848137130255 24.32869364651322 34.77057366123353 23.158884555123 36 38.260369755428734 23.59723218824271 22.64872003475765 15.493678021570915 37 16.603055079306277 41.530099706701556 27.39989636706245 14.46694884692972 38 17.554398061251746 40.84663925275975 22.649089273252486 18.94987341273602 39 18.552942030787108 40.081515551710005 24.918552142014047 16.44699027548884 40 28.095541691333846 24.115704574741873 23.538584807312894 24.250168926611387 41 13.602623070267317 22.179787146315675 27.33761814093341 36.879971642483596 42 18.096747870416984 21.75565519524716 24.31589337869223 35.83170355564362 43 18.175272590318812 23.68412631402749 26.70892806372564 31.431673031928053 44 14.630890738636992 30.918800762600572 33.4283301927548 21.021978306007632 45 12.734297209910853 49.16607485941245 19.988048980717135 18.11157894995957 46 19.059598785451513 40.817592491165975 23.674279954165193 16.448528769217326 47 17.940067669108153 28.067910343970276 26.342089619105874 27.649932367815694 48 19.925093817347562 23.5512619956356 37.90602387988425 18.61762030713258 49 20.642893451309135 24.24413803119573 37.67844988756688 17.434518629928256 50 17.27186907295291 42.77406419580471 23.579324121243154 16.374742609999224 51 15.506724448388459 40.428292038110335 22.843739499780302 21.221244013720902 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6046.0 1 4991.0 2 3936.0 3 6532.5 4 9129.0 5 6587.5 6 4046.0 7 4716.5 8 5387.0 9 5808.0 10 6229.0 11 6728.5 12 7228.0 13 6986.0 14 6744.0 15 6429.0 16 6114.0 17 5853.0 18 5592.0 19 5116.5 20 4641.0 21 4449.5 22 4258.0 23 5542.5 24 6827.0 25 7207.0 26 9714.0 27 11841.0 28 15814.0 29 19787.0 30 22893.5 31 26000.0 32 30587.5 33 35175.0 34 41235.0 35 47295.0 36 49581.0 37 51867.0 38 71951.0 39 92035.0 40 127658.5 41 163282.0 42 200332.0 43 237382.0 44 228193.0 45 219004.0 46 194766.5 47 170529.0 48 144734.5 49 118940.0 50 108734.0 51 98528.0 52 85459.0 53 72390.0 54 59320.0 55 46250.0 56 39116.5 57 31983.0 58 29325.5 59 26668.0 60 23241.0 61 19814.0 62 17751.0 63 15688.0 64 14077.0 65 12466.0 66 10545.0 67 8624.0 68 6838.0 69 5052.0 70 4864.0 71 4676.0 72 3671.0 73 2666.0 74 2119.5 75 1175.0 76 777.0 77 561.5 78 346.0 79 390.5 80 435.0 81 255.5 82 76.0 83 45.5 84 15.0 85 19.0 86 23.0 87 15.5 88 8.0 89 5.0 90 2.0 91 2.5 92 3.0 93 1.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1624966.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.980424180885318 #Duplication Level Percentage of deduplicated Percentage of total 1 79.6452467620776 11.931195804872054 2 7.994497533396919 2.395219283266545 3 2.8440223501265622 1.2781398355444276 4 1.3816571147700467 0.827912386071738 5 0.799084407201367 0.5985311688103885 6 0.5172734889216825 0.4649385768943971 7 0.37133729498469603 0.3893953135137299 8 0.2825726180225125 0.33864461439044136 9 0.21418371016288218 0.2887706547788196 >10 2.853230602393524 10.572417790574713 >50 1.4476179482047193 15.608793073177033 >100 1.6098765592095092 50.69709893188755 >500 0.03728135189748337 3.416882746586716 >1k 0.0012709551783232967 0.49223318751326317 >5k 8.473034522155312E-4 0.6998266321181991 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5620 0.34585339016324035 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5407 0.33274542359655523 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 3770 0.23200485425541212 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2371 0.14591074520943823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.024246661160910444 0.0 2 0.0 0.0 0.0 0.06941683702920554 0.0 3 0.0 0.0 0.0 0.09637124715224811 0.0 4 0.0 0.0 0.0 0.1321258414022201 0.0 5 6.153974913936661E-5 0.0 0.0 0.23982040239611166 0.0 6 6.153974913936661E-5 0.0 0.0 0.3374224445311471 0.0 7 6.153974913936661E-5 0.0 0.0 0.4128701769760106 0.0 8 6.153974913936661E-5 0.0 0.0 0.6137974579160426 0.0 9 6.153974913936661E-5 0.0 0.0 0.6977376757421386 0.0 10 6.153974913936661E-5 0.0 0.0 0.835279015068623 0.0 11 6.153974913936661E-5 0.0 0.0 0.9565738606223145 0.0 12 6.153974913936661E-5 0.0 0.0 1.0775610074303092 0.0 13 6.153974913936661E-5 0.0 0.0 1.122669643549465 0.0 14 6.153974913936661E-5 0.0 0.0 1.136946865349798 0.0 15 6.153974913936661E-5 0.0 0.0 1.159778112280503 0.0 16 6.153974913936661E-5 0.0 0.0 1.2185485727085983 0.0 17 6.153974913936661E-5 0.0 0.0 1.2888885059748942 0.0 18 6.153974913936661E-5 0.0 0.0 1.4139988159752266 0.0 19 6.153974913936661E-5 0.0 0.0 1.4558458453899958 0.0 20 1.2307949827873322E-4 0.0 0.0 1.5068007576773914 0.0 21 2.4615899655746644E-4 0.0 0.0 1.5797253604075407 0.0 22 2.4615899655746644E-4 0.0 0.0 1.6549269338558468 0.0 23 2.4615899655746644E-4 0.0 0.0 1.748959670540799 0.0 24 3.07698745696833E-4 0.0 0.0 1.8178841895768896 0.0 25 3.07698745696833E-4 0.0 0.0 1.8750546165273612 0.0 26 3.07698745696833E-4 0.0 0.0 1.9354251104330797 0.0 27 3.07698745696833E-4 0.0 0.0 1.9902570269162554 0.0 28 3.07698745696833E-4 0.0 0.0 2.0483505501038177 0.0 29 3.07698745696833E-4 0.0 0.0 2.1136442239406854 0.0 30 3.07698745696833E-4 0.0 0.0 2.2155540485154765 0.0 31 3.07698745696833E-4 0.0 0.0 2.301217379317475 0.0 32 3.07698745696833E-4 0.0 0.0 2.376111254020084 0.0 33 3.07698745696833E-4 0.0 0.0 2.452974400695153 0.0 34 3.07698745696833E-4 0.0 0.0 2.539253128988545 0.0 35 3.6923849483619966E-4 0.0 0.0 2.6741482591020365 0.0 36 3.6923849483619966E-4 0.0 0.0 2.7641193723437905 0.0 37 3.6923849483619966E-4 0.0 0.0 2.8580905692796033 0.0 38 3.6923849483619966E-4 0.0 0.0 2.941415389614306 0.0 39 4.3077824397556625E-4 0.0 0.0 3.034463490313028 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAG 60 0.0 45.000004 1 ACACGTG 20 7.0341484E-4 45.000004 14 ACCGGAT 20 7.0341484E-4 45.000004 15 ACACCGC 20 7.0341484E-4 45.000004 31 TGGAACG 65 0.0 45.000004 1 TTGTACG 40 6.8175723E-9 45.000004 1 CACGACA 60 0.0 45.000004 14 TCTAGCG 40 6.8175723E-9 45.000004 1 TGCGCAT 20 7.0341484E-4 45.000004 1 TACGATG 30 2.166058E-6 45.000004 1 CGTACTG 20 7.0341484E-4 45.000004 11 AGACACG 20 7.0341484E-4 45.000004 22 CATACGT 20 7.0341484E-4 45.000004 21 ACGCTAT 40 6.8175723E-9 45.000004 35 TCCGTCG 30 2.166058E-6 45.000004 2 CAAGCCG 20 7.0341484E-4 45.000004 45 GTTCACG 75 0.0 45.000004 1 TTAGTCA 20 7.0341484E-4 45.000004 44 CGTAAGA 20 7.0341484E-4 45.000004 22 AACCTCG 30 2.166058E-6 45.000004 22 >>END_MODULE