##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545212_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1230380
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33989580454819	31.0	31.0	34.0	30.0	34.0
2	31.58695768786879	31.0	31.0	34.0	30.0	34.0
3	31.65891025536826	31.0	31.0	34.0	30.0	34.0
4	35.41862920398576	37.0	35.0	37.0	33.0	37.0
5	35.29091418911231	37.0	35.0	37.0	33.0	37.0
6	35.278142525073555	37.0	35.0	37.0	32.0	37.0
7	35.89035013573042	37.0	35.0	37.0	35.0	37.0
8	35.99519091662739	37.0	35.0	37.0	35.0	37.0
9	37.659292251174435	39.0	37.0	39.0	35.0	39.0
10	36.945760659308505	39.0	37.0	39.0	32.0	39.0
11	36.56304068661714	39.0	35.0	39.0	32.0	39.0
12	35.54315983679839	37.0	35.0	39.0	31.0	39.0
13	35.019941806596336	37.0	34.0	39.0	30.0	39.0
14	35.79868333360425	38.0	34.0	40.0	29.0	41.0
15	36.27838716494091	38.0	35.0	40.0	31.0	41.0
16	36.233969180253254	37.0	35.0	40.0	31.0	41.0
17	36.33393910824298	37.0	35.0	40.0	31.0	41.0
18	36.411126643801104	37.0	35.0	40.0	31.0	41.0
19	36.32749150668899	37.0	35.0	40.0	31.0	41.0
20	36.18192103252654	36.0	35.0	40.0	31.0	41.0
21	36.04907345698077	36.0	34.0	40.0	31.0	41.0
22	35.94624912628619	36.0	34.0	40.0	30.0	41.0
23	35.904724556641035	36.0	34.0	40.0	31.0	41.0
24	35.70847217932671	36.0	34.0	40.0	30.0	41.0
25	35.66771485232205	35.0	34.0	40.0	30.0	41.0
26	35.50290885742616	35.0	34.0	40.0	30.0	41.0
27	35.46491002779629	35.0	34.0	40.0	30.0	41.0
28	35.54423836538305	36.0	34.0	40.0	30.0	41.0
29	35.56678180724654	36.0	34.0	40.0	30.0	41.0
30	35.502381378110826	36.0	34.0	40.0	30.0	41.0
31	35.34712609112632	36.0	34.0	40.0	30.0	41.0
32	35.18174872803524	35.0	34.0	40.0	29.0	41.0
33	35.00847543035485	35.0	34.0	40.0	29.0	41.0
34	34.873286301792945	35.0	34.0	40.0	27.0	41.0
35	34.687898047757606	35.0	34.0	40.0	27.0	41.0
36	34.46023504933435	35.0	33.0	40.0	25.0	41.0
37	34.27496383231197	35.0	33.0	40.0	25.0	41.0
38	34.36015133535981	35.0	33.0	40.0	26.0	41.0
39	34.371166631447196	35.0	33.0	40.0	26.0	41.0
40	34.18945366472147	35.0	33.0	40.0	24.0	41.0
41	34.23784034200816	35.0	33.0	40.0	25.0	41.0
42	34.173307433475834	35.0	33.0	40.0	25.0	41.0
43	34.039084673027844	35.0	33.0	40.0	24.0	41.0
44	33.98536712235244	35.0	33.0	39.0	24.0	41.0
45	33.90126952648775	35.0	33.0	39.0	24.0	41.0
46	33.843411791479056	35.0	33.0	39.0	23.0	41.0
47	33.897458508753395	35.0	33.0	39.0	24.0	41.0
48	33.87030998553293	35.0	33.0	39.0	24.0	41.0
49	33.859731139973015	35.0	33.0	39.0	24.0	41.0
50	33.637694045741966	35.0	33.0	39.0	24.0	41.0
51	33.46919488288171	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	12.0
15	29.0
16	90.0
17	263.0
18	585.0
19	1128.0
20	2087.0
21	3337.0
22	5021.0
23	7703.0
24	10674.0
25	14932.0
26	19625.0
27	23237.0
28	26373.0
29	30660.0
30	37482.0
31	46762.0
32	58870.0
33	78924.0
34	135673.0
35	201597.0
36	86953.0
37	101730.0
38	139116.0
39	197374.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.45509517384873	24.0019343617419	29.08898064012744	14.45398982428193
2	31.0262683073522	26.652335050959863	29.479754222272792	12.84164241941514
3	29.331751166306347	25.9097189486175	31.883239324436353	12.875290560639801
4	26.39802337489231	27.828150652643895	30.565028690323317	15.208797282140477
5	23.15674832165672	33.00045514393927	29.514377671938753	14.328418862465256
6	21.457842292625042	41.26359336140054	26.780831938100423	10.49773240787399
7	86.1035615013248	5.472536939807214	6.729465693525577	1.6944358653424145
8	88.10099318909606	4.109218290284303	6.180529592483623	1.6092589281360232
9	83.89879549407499	5.792519384255271	7.61919081909654	2.6894943025731886
10	54.865569986508234	21.781482143727953	12.91600968806385	10.436938181699963
11	49.705131747915274	19.894585412636747	18.72454038589704	11.675742453550935
12	44.8501276028544	21.57089679611177	21.88299549732603	11.695980103707798
13	20.429948471204018	43.663502332612694	22.35252523610592	13.554023960077375
14	14.063378793543457	45.757895934589314	26.175490498870268	14.003234772996962
15	13.348640257481428	23.198280206115186	50.46384043953901	12.989239096864383
16	14.873453729742032	17.819860530892896	48.84710414668639	18.459581592678685
17	14.646613241437606	18.54093857182334	29.46049188055723	37.35195630618183
18	19.315008371397454	23.126920138493798	38.297111461499696	19.26096002860905
19	32.747525154830214	22.602366748484208	25.901347551163056	18.748760545522522
20	34.07085615826005	22.225166208813537	25.449373364326465	18.254604268599945
21	21.393634486906485	30.725060550399064	27.821648596368604	20.05965636632585
22	21.49587932183553	25.710756026593412	22.862205172385767	29.931159479185293
23	18.46120710674751	32.801004567694534	24.808189339878737	23.92959898567922
24	17.168029389294365	22.95559095563972	43.46437685918171	16.4120027958842
25	16.362912271005705	26.316341292933892	39.1600968806385	18.1606495554219
26	14.859636860157025	38.24517628700077	27.15104276727515	19.74414408556706
27	16.189551195565596	39.635722297176486	27.580828687072284	16.59389782018563
28	14.270225458801347	30.234642955834783	39.65791056421593	15.837221021147938
29	14.619304605081357	26.156715811375346	38.12114956354947	21.102830019993824
30	18.104650595750908	35.11598042881061	29.573383832637074	17.20598514280141
31	31.411677693070434	28.27500447016369	23.63871324306312	16.674604593702757
32	33.529478697638126	24.80445065752044	26.10730018368309	15.55877046115834
33	31.08104813147158	26.98613436499293	23.304832653326617	18.62798485020888
34	19.699605000081274	26.710040800403128	26.92371462475008	26.666639574765522
35	21.059510070059655	24.79916773679676	32.13186170126303	22.009460491880557
36	33.23607340821535	26.736536679724964	23.602951933548987	16.424437978510703
37	19.76356897868951	38.04418147239064	26.71507989401648	15.477169654903363
38	19.544774785025766	37.680879078008424	23.246720525366147	19.52762561159967
39	20.88200393374405	35.58705440595588	25.44660999040947	18.084331669890602
40	26.470683853768755	25.572587330743346	25.412311643557274	22.544417171930622
41	16.254246655504804	24.957899185617453	27.219070531055443	31.5687836278223
42	19.394008355142315	24.830215055511307	24.75747330093142	31.018303288414963
43	19.893772655602334	25.09297940473675	27.32521659974967	27.688031339911245
44	17.230124026723452	30.005120369316796	31.66078772411775	21.103967879842
45	15.762609925388904	42.66576179716836	21.911279442123572	19.66034883531917
46	20.10850306409402	37.8739088736813	23.925372649100275	18.0922154131244
47	20.641265300151172	28.512492075618916	26.08600594938149	24.76023667484842
48	21.003998764609307	25.39109868495912	34.43001349176677	19.174889058664803
49	20.68661714267137	26.077309449113283	34.50941985402884	18.726653554186512
50	18.98494773972269	38.091971586014076	25.247078138461287	17.676002535801945
51	17.983305970513175	36.34373120499358	24.35759684000065	21.315365984492594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3855.0
1	3576.5
2	3298.0
3	6735.0
4	10172.0
5	7491.5
6	4811.0
7	5020.5
8	5230.0
9	5680.5
10	6131.0
11	6146.0
12	6161.0
13	6207.0
14	6253.0
15	5965.5
16	5678.0
17	5605.0
18	5532.0
19	5256.5
20	4981.0
21	5122.5
22	5264.0
23	5219.5
24	5175.0
25	6589.0
26	10727.0
27	13451.0
28	14987.0
29	16523.0
30	19779.0
31	23035.0
32	25218.0
33	27401.0
34	30856.0
35	34311.0
36	37207.5
37	40104.0
38	49443.5
39	58783.0
40	79513.0
41	100243.0
42	120944.0
43	141645.0
44	139558.0
45	137471.0
46	125009.5
47	112548.0
48	98111.0
49	83674.0
50	79088.5
51	74503.0
52	65588.0
53	56673.0
54	50345.5
55	44018.0
56	40810.0
57	37602.0
58	35292.0
59	32982.0
60	32390.0
61	31798.0
62	28363.0
63	24928.0
64	21977.5
65	19027.0
66	15480.5
67	11934.0
68	10612.0
69	9290.0
70	7995.5
71	6701.0
72	5780.5
73	4860.0
74	3977.5
75	2394.0
76	1693.0
77	1221.5
78	750.0
79	583.0
80	416.0
81	279.5
82	143.0
83	160.5
84	178.0
85	106.5
86	35.0
87	25.0
88	15.0
89	8.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1230380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.41763205569915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.43373788704834	14.445637251568064
2	7.120493192229812	2.6228524733919873
3	2.5665902745613858	1.4181154594382237
4	1.3287108803617167	0.9788683241162477
5	0.8488692054883	0.781708034504859
6	0.5984661231409394	0.6613397312286337
7	0.47629756401491985	0.6140591298136824
8	0.39753303475613955	0.5857293731299232
9	0.3385682675638477	0.5612063199953804
>10	5.349300217739877	24.48404130716982
>50	1.41837918740572	18.321513674163445
>100	1.1134981942629965	32.49013638821811
>500	0.006370647617360829	0.7181571406367296
>1k	0.0027302775502974982	0.8223314113581037
>5k	4.550462583829163E-4	0.49430398126682595
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5898	0.47936409889627596	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3401	0.27641866740356635	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1603	0.13028495261626488	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1407	0.11435491474178709	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1237	0.10053804515678083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.127570344121329E-5	0.0	0.0	0.017718103350184495	0.0
2	8.127570344121329E-5	0.0	0.0	0.06315122157382272	0.0
3	8.127570344121329E-5	0.0	0.0	0.09102878785415888	0.0
4	8.127570344121329E-5	0.0	0.0	0.126139891740763	0.0
5	8.127570344121329E-5	0.0	0.0	0.21765633381556918	0.0
6	8.127570344121329E-5	0.0	0.0	0.3106357385523172	0.0
7	8.127570344121329E-5	0.0	0.0	0.3813456005461727	0.0
8	8.127570344121329E-5	0.0	0.0	0.5600708724134007	0.0
9	8.127570344121329E-5	0.0	0.0	0.6543506884052082	0.0
10	8.127570344121329E-5	0.0	0.0	0.8266551797005803	0.0
11	8.127570344121329E-5	0.0	0.0	1.007981274077927	0.0
12	8.127570344121329E-5	0.0	0.0	1.1606170451405258	0.0
13	8.127570344121329E-5	0.0	0.0	1.2204359628732586	0.0
14	8.127570344121329E-5	0.0	0.0	1.2478258749329476	0.0
15	8.127570344121329E-5	0.0	0.0	1.287244591101936	0.0
16	8.127570344121329E-5	0.0	0.0	1.3674637103984135	0.0
17	8.127570344121329E-5	0.0	0.0	1.4589801524732195	0.0
18	8.127570344121329E-5	0.0	0.0	1.6116159235358183	0.0
19	8.127570344121329E-5	0.0	0.0	1.6752547993302882	0.0
20	8.127570344121329E-5	0.0	0.0	1.743445114517466	0.0
21	8.127570344121329E-5	0.0	0.0	1.8343926266681838	0.0
22	8.127570344121329E-5	0.0	0.0	1.921357629350282	0.0
23	8.127570344121329E-5	0.0	0.0	2.035387441278304	0.0
24	8.127570344121329E-5	0.0	0.0	2.121783514036314	0.0
25	1.6255140688242658E-4	0.0	0.0	2.1898112778166094	0.0
26	1.6255140688242658E-4	0.0	0.0	2.2599522098863765	0.0
27	1.6255140688242658E-4	0.0	0.0	2.3280612493701134	0.0
28	2.4382711032363985E-4	0.0	0.0	2.402997447942912	0.0
29	2.4382711032363985E-4	0.0	0.0	2.484679529901331	0.0
30	2.4382711032363985E-4	0.0	0.0	2.5910694257058795	0.0
31	2.4382711032363985E-4	0.0	0.0	2.694127017669338	0.0
32	2.4382711032363985E-4	0.0	0.0	2.785155805523497	0.0
33	2.4382711032363985E-4	0.0	0.0	2.8772411775223916	0.0
34	2.4382711032363985E-4	0.0	0.0	2.97225247484517	0.0
35	2.4382711032363985E-4	0.0	0.0	3.1090394837367317	0.0
36	2.4382711032363985E-4	0.0	0.0	3.223069295664754	0.0
37	2.4382711032363985E-4	0.0	0.0	3.3345795607860986	0.0
38	2.4382711032363985E-4	0.0	0.0	3.439587769632146	0.0
39	2.4382711032363985E-4	0.0	0.0	3.554917992815228	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	25	3.8909668E-5	45.0	1
GGTCGTA	20	7.03348E-4	45.0	8
ATTCGAC	20	7.03348E-4	45.0	14
ATTCGAA	25	3.8909668E-5	45.0	25
CCCGTAG	25	3.8909668E-5	45.0	21
TACGCGC	20	7.03348E-4	45.0	34
TGCGCAC	20	7.03348E-4	45.0	1
CGGATCG	20	7.03348E-4	45.0	30
GTATACG	40	6.8157533E-9	45.0	1
TTACGCG	25	3.8909668E-5	45.0	1
TCGAGGT	25	3.8909668E-5	45.0	3
GTGCGCT	20	7.03348E-4	45.0	9
AATCGAC	20	7.03348E-4	45.0	43
CGTATGA	30	2.165627E-6	44.999996	15
ATCTACG	30	2.165627E-6	44.999996	1
ACGTTAG	65	0.0	44.999996	1
GCCACGA	30	2.165627E-6	44.999996	43
TCGATAG	105	0.0	42.857147	1
TATACGG	160	0.0	42.1875	2
TACGGGT	75	0.0	42.000004	4
>>END_MODULE