FastQCFastQC Report
Thu 26 May 2016
SRR1545211_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545211_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1535211
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66180.43108080908748053No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60810.3961019039076713No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51200.33350464528980056No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT33770.2199697631139954No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20340.13248993135145593No Hit
TGTAATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA16740.10904038597951682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTA302.1659798E-645.00000439
CGTCTAC302.1659798E-645.00000418
TCTAGTC850.045.00000437
CCGTCTA302.1659798E-645.00000417
CGACTGG850.045.0000042
GACTACG302.1659798E-645.0000041
CGACGGT502.1827873E-1145.028
ACGTTAG351.2122837E-745.01
CTATCGA207.034029E-445.023
GCAACCG207.034029E-445.08
CTATACG351.2122837E-745.01
CACGCGT207.034029E-445.038
GAATCGT207.034029E-445.08
GCCGGAC207.034029E-445.018
GTTAGCG406.8175723E-945.01
CGATTAC207.034029E-445.010
AGGCGAT453.8562575E-1045.06
CGTTATC207.034029E-445.044
ACGATTG406.8175723E-945.01
CCACTTG253.8914204E-545.01