##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545211_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1535211
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272197111667385	31.0	31.0	34.0	30.0	34.0
2	31.52714447720867	31.0	31.0	34.0	30.0	34.0
3	31.602775774795777	31.0	31.0	34.0	30.0	34.0
4	35.340781169493965	37.0	35.0	37.0	33.0	37.0
5	35.18794354652227	37.0	35.0	37.0	32.0	37.0
6	35.16564172612104	37.0	35.0	37.0	32.0	37.0
7	35.837851604763124	37.0	35.0	37.0	35.0	37.0
8	35.9562737630202	37.0	35.0	37.0	35.0	37.0
9	37.65628242632446	39.0	37.0	39.0	35.0	39.0
10	36.92307572053613	39.0	37.0	39.0	32.0	39.0
11	36.44093678328256	38.0	35.0	39.0	32.0	39.0
12	34.830588108084164	35.0	34.0	39.0	30.0	39.0
13	34.02677547255719	35.0	33.0	39.0	25.0	39.0
14	34.638499854417404	35.0	33.0	40.0	25.0	41.0
15	35.32401279042425	35.0	33.0	40.0	30.0	41.0
16	35.43576094751796	35.0	34.0	40.0	31.0	41.0
17	35.56428595157278	35.0	34.0	40.0	31.0	41.0
18	35.69031813867931	36.0	34.0	39.0	31.0	41.0
19	35.577729706209766	36.0	34.0	39.0	31.0	41.0
20	35.370892339880314	35.0	34.0	39.0	30.0	41.0
21	35.189546583498945	35.0	34.0	39.0	30.0	41.0
22	35.061230019847436	35.0	34.0	39.0	30.0	41.0
23	35.08528599651774	35.0	34.0	39.0	30.0	41.0
24	34.89818142261878	35.0	34.0	39.0	29.0	41.0
25	34.894261440284104	35.0	34.0	39.0	29.0	41.0
26	34.751471296128024	35.0	34.0	39.0	29.0	41.0
27	34.64851215891496	35.0	34.0	39.0	29.0	41.0
28	34.797038973795786	35.0	34.0	39.0	29.0	41.0
29	34.887591998754566	35.0	34.0	39.0	29.0	41.0
30	34.84038545841582	35.0	34.0	39.0	29.0	41.0
31	34.60468430723855	35.0	34.0	39.0	29.0	41.0
32	34.422545825948355	35.0	34.0	39.0	29.0	41.0
33	34.213385000498306	35.0	33.0	39.0	27.0	41.0
34	34.08746745561359	35.0	34.0	39.0	27.0	41.0
35	33.92063436231241	35.0	33.0	39.0	26.0	41.0
36	33.5933516630613	35.0	33.0	38.0	23.0	41.0
37	33.36156984284245	35.0	33.0	38.0	23.0	41.0
38	33.551392609875776	35.0	33.0	38.0	24.0	41.0
39	33.59782270971222	35.0	33.0	38.0	24.0	41.0
40	33.35163244661483	35.0	32.0	38.0	23.0	41.0
41	33.52308575173054	35.0	33.0	38.0	23.0	41.0
42	33.475264312202036	35.0	33.0	38.0	23.0	41.0
43	33.335306352025874	35.0	33.0	38.0	23.0	41.0
44	33.30213241046345	35.0	33.0	38.0	23.0	40.0
45	33.25040401612547	35.0	32.0	38.0	23.0	40.0
46	33.21188683509954	35.0	33.0	38.0	23.0	40.0
47	33.26055245826144	35.0	33.0	38.0	23.0	40.0
48	33.28619518750191	35.0	33.0	38.0	23.0	40.0
49	33.330952553101824	35.0	33.0	38.0	24.0	40.0
50	33.06367333219994	35.0	32.0	37.0	24.0	40.0
51	32.867927600831415	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	5.0
14	18.0
15	41.0
16	168.0
17	431.0
18	1046.0
19	2012.0
20	3581.0
21	5445.0
22	8137.0
23	11715.0
24	16461.0
25	22759.0
26	29112.0
27	33575.0
28	37664.0
29	43824.0
30	52676.0
31	64950.0
32	82914.0
33	111414.0
34	202037.0
35	314473.0
36	83909.0
37	93289.0
38	124096.0
39	189218.0
40	237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.12035479162148	25.25229431003295	31.01176320388533	13.61558769446024
2	28.594701314672704	26.273131185224702	31.891577118715276	13.240590381387316
3	26.67620281511792	26.405751391828225	32.14932670492851	14.768719088125346
4	24.403225354690658	29.290436298332935	31.087778813466034	15.218559533510378
5	21.452751445892453	33.95194536777029	30.4440887930063	14.15121439333095
6	20.32333014810342	39.60282983902538	29.16915003865918	10.904689974212014
7	86.78461787988752	5.5791679449925775	5.953839569935338	1.6823746051845643
8	89.49154220494773	3.8988126062150417	5.331644966066554	1.278000222770681
9	86.57826188061446	4.60138704060875	6.7727498044242775	2.0476012743525156
10	65.8056775257603	17.235936949383504	9.97061641689644	6.987769107959753
11	61.49499971013756	15.085092537768425	14.771780556548904	8.648127195545108
12	54.625194842923875	19.61372085009813	17.88594531956845	7.8751389874095485
13	18.213327028011133	56.62550620077631	16.482294616179797	8.678872155032762
14	10.712078014031947	55.866587719863915	23.390139856996857	10.031194409107282
15	7.901845414083146	21.310556008262058	61.41950520156513	9.368093376089671
16	9.4854062405754	14.257584136643105	57.880773392061414	18.376236230720078
17	9.629881495116958	14.663391546829718	28.393360912604194	47.31336604544913
18	17.058567193695197	20.945720164850304	43.55355713318886	18.44215550826564
19	34.25913441214269	21.058538533139746	24.31164185248803	20.37068520222953
20	35.730658521857904	20.05470257834265	25.570361337952896	18.644277561846547
21	17.835268246514648	33.7194040428319	28.123235177444666	20.322092533208792
22	20.477445771297887	24.65934650025306	19.70445756316233	35.158750165286726
23	13.992278585810029	35.10637951395606	22.47808281728049	28.423259082953418
24	14.287352031740264	20.368731073448533	51.85560812161977	13.488308773191438
25	11.136123959507845	25.69138704712251	45.35708772279511	17.815401270574533
26	12.028704849040295	41.72325497928298	26.90652946077119	19.34151071090554
27	11.607068995727623	46.783797145799504	25.81957789515578	15.789555963317095
28	8.921965775388529	32.9862149242026	43.40308921705225	14.688730083356619
29	8.780096025888298	25.215231000820083	42.45527162064368	23.54940135264794
30	14.591870433445306	40.14764094316677	31.029806326296516	14.230682297091409
31	34.04098850255763	29.627914338810758	22.39509748171424	13.935999676917374
32	36.812203664512566	22.976971895068495	26.23815228004489	13.972672160374048
33	35.181092371016106	27.60708462875787	19.421629990926327	17.7901930092997
34	17.611259950586597	26.374876157088504	24.974352059749442	31.039511832575457
35	18.16597197388502	24.309101485072738	34.700962929525645	22.823963611516593
36	38.651234260306886	23.710095876071758	23.091874667390996	14.546795196230356
37	17.45297551932601	40.700919938692465	27.923979179409216	13.92212536257231
38	17.257497503600483	40.61715295161382	22.30208095173888	19.82326859304682
39	19.21905197396319	39.538734415008754	25.283690645780936	15.958522965247122
40	27.611644262580192	23.43514995658577	24.2844794624322	24.668726318401834
41	13.827936355328355	23.11578017614517	26.442684425789025	36.61359904273745
42	18.4181197242594	22.47580300036933	23.74924358931769	35.35683368605358
43	18.21717014794709	22.551883747576067	28.11782875448391	31.11311734999293
44	13.862459297125932	31.10477973386069	33.21999386403563	21.812767104977752
45	12.613705868444141	49.793546294287886	19.228822617868165	18.36392521939981
46	19.64498691059405	40.22046480907185	23.563666492749206	16.5708817875849
47	18.56220415304476	29.46930421942	25.18858971177252	26.77990191576272
48	20.719757740141258	24.18833632640725	36.15216409991851	18.93974183353298
49	21.574037705566205	23.477749964011462	37.0956826130089	17.852529717413436
50	18.204663723748723	42.73184598078049	23.654468343439436	15.409021952031349
51	15.33098707604362	39.73590600901114	22.703654416233338	22.229452498711904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7523.0
1	5997.0
2	4471.0
3	7277.0
4	10083.0
5	7363.5
6	4644.0
7	5140.0
8	5636.0
9	6380.5
10	7125.0
11	7525.5
12	7926.0
13	7959.5
14	7993.0
15	7442.0
16	6891.0
17	6388.5
18	5886.0
19	5708.0
20	5530.0
21	5022.5
22	4515.0
23	4730.0
24	4945.0
25	6413.5
26	8768.5
27	9655.0
28	12394.0
29	15133.0
30	18300.5
31	21468.0
32	24634.0
33	27800.0
34	33615.0
35	39430.0
36	41183.5
37	42937.0
38	60975.0
39	79013.0
40	113715.0
41	148417.0
42	188275.5
43	228134.0
44	219048.5
45	209963.0
46	191376.0
47	172789.0
48	148042.0
49	123295.0
50	106947.0
51	90599.0
52	78512.0
53	66425.0
54	53804.5
55	41184.0
56	35802.5
57	30421.0
58	27476.0
59	24531.0
60	22349.5
61	20168.0
62	18982.5
63	17797.0
64	14755.5
65	11714.0
66	9664.5
67	7615.0
68	6427.0
69	5239.0
70	4635.5
71	4032.0
72	3449.0
73	2866.0
74	2098.0
75	1026.0
76	722.0
77	645.5
78	569.0
79	539.5
80	510.0
81	396.5
82	283.0
83	157.5
84	32.0
85	55.0
86	78.0
87	44.0
88	10.0
89	6.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1535211.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.977941559389382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.25032639725998	12.662570837379539
2	8.377863976837869	2.67722042028858
3	3.0038217979790964	1.4398466742878984
4	1.4686739666551019	0.9386554723604725
5	0.850615158936467	0.679553964950879
6	0.5426484062384049	0.5202242713303814
7	0.38768089432433644	0.43360398712442416
8	0.3027404632378578	0.38697355434215697
9	0.2403604085120848	0.3456418104357345
>10	2.7674493488617244	10.761557097583282
>50	1.345580063624959	15.471098427987581
>100	1.4260200610495009	48.40747567136994
>500	0.031165234647862687	3.1391434184439007
>1k	0.003790366376091408	0.9373690850787468
>5k	0.0012634554586971362	1.1990653070364816
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6618	0.43108080908748053	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6081	0.3961019039076713	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5120	0.33350464528980056	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3377	0.2199697631139954	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2034	0.13248993135145593	No Hit
TGTAATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1674	0.10904038597951682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021039453208712026	0.0
2	0.0	0.0	0.0	0.10480644028736115	0.0
3	0.0	0.0	0.0	0.16935782768622684	0.0
4	0.0	0.0	0.0	0.2399670143061768	0.0
5	0.0	0.0	0.0	0.43733402118666426	0.0
6	0.0	0.0	0.0	0.6201101998357229	0.0
7	0.0	0.0	0.0	0.7466074695921278	0.0
8	0.0	0.0	0.0	1.0350368776669787	0.0
9	0.0	0.0	0.0	1.168113047652733	0.0
10	0.0	0.0	0.0	1.376032349950593	0.0
11	0.0	0.0	0.0	1.5905305524778026	0.0
12	0.0	0.0	0.0	1.7753259975338895	0.0
13	0.0	0.0	0.0	1.8503645427240947	0.0
14	0.0	0.0	0.0	1.8773966575278578	0.0
15	0.0	0.0	0.0	1.910486571552705	0.0
16	0.0	0.0	0.0	2.0044150282925277	0.0
17	0.0	0.0	0.0	2.119904039249328	0.0
18	0.0	0.0	0.0	2.3136884766979913	0.0
19	0.0	0.0	0.0	2.3815618830245486	0.0
20	0.0	0.0	0.0	2.467869237518491	0.0
21	0.0	0.0	0.0	2.5752160452211457	0.0
22	0.0	0.0	0.0	2.6729224842708916	0.0
23	0.0	0.0	0.0	2.8113399395913654	0.0
24	1.3027525206632835E-4	0.0	0.0	2.900708762508867	0.0
25	1.3027525206632835E-4	0.0	0.0	2.972229875893281	0.0
26	1.3027525206632835E-4	0.0	0.0	3.0538473213128356	0.0
27	1.3027525206632835E-4	0.0	0.0	3.1262803614617143	0.0
28	1.3027525206632835E-4	0.0	0.0	3.2046409255796107	0.0
29	1.3027525206632835E-4	0.0	0.0	3.2860629581210663	0.0
30	1.3027525206632835E-4	0.0	0.0	3.422461147034512	0.0
31	1.3027525206632835E-4	0.0	0.0	3.5410116264148708	0.0
32	1.3027525206632835E-4	0.0	0.0	3.6396299922290813	0.0
33	1.3027525206632835E-4	0.0	0.0	3.7360336787581643	0.0
34	1.3027525206632835E-4	0.0	0.0	3.848917184673638	0.0
35	1.3027525206632835E-4	0.0	0.0	4.028306206768971	0.0
36	1.3027525206632835E-4	0.0	0.0	4.140082373041881	0.0
37	1.3027525206632835E-4	0.0	0.0	4.251858539314791	0.0
38	1.954128780994925E-4	0.0	0.0	4.356730117228185	0.0
39	1.954128780994925E-4	0.0	0.0	4.459061327726286	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGTA	30	2.1659798E-6	45.000004	39
CGTCTAC	30	2.1659798E-6	45.000004	18
TCTAGTC	85	0.0	45.000004	37
CCGTCTA	30	2.1659798E-6	45.000004	17
CGACTGG	85	0.0	45.000004	2
GACTACG	30	2.1659798E-6	45.000004	1
CGACGGT	50	2.1827873E-11	45.0	28
ACGTTAG	35	1.2122837E-7	45.0	1
CTATCGA	20	7.034029E-4	45.0	23
GCAACCG	20	7.034029E-4	45.0	8
CTATACG	35	1.2122837E-7	45.0	1
CACGCGT	20	7.034029E-4	45.0	38
GAATCGT	20	7.034029E-4	45.0	8
GCCGGAC	20	7.034029E-4	45.0	18
GTTAGCG	40	6.8175723E-9	45.0	1
CGATTAC	20	7.034029E-4	45.0	10
AGGCGAT	45	3.8562575E-10	45.0	6
CGTTATC	20	7.034029E-4	45.0	44
ACGATTG	40	6.8175723E-9	45.0	1
CCACTTG	25	3.8914204E-5	45.0	1
>>END_MODULE