FastQCFastQC Report
Thu 26 May 2016
SRR1545210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545210_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3470407
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360191.0378897921771135No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104280.30048348795976954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT239700.042.071341
TACCGGT556.184564E-1140.90909240
TATCGTG850.039.7058831
AGGGCGA29550.038.299496
CGCATCG2600.038.07692721
GGGCGAT56000.038.008937
TAGGGAC24050.037.515595
ACGGGCT9800.037.423475
GACCGAT15400.037.2564939
GGTACCT24300.036.9444438
GTTGATC23550.036.8789816
CGAATAT1600.036.562514
TCACGAC2650.036.50943425
TATACGG3150.036.428572
GCCAGTA24350.036.12936425
GGGTACC25700.035.982497
TTGATCC24150.035.86956817
GCGCGAC20200.035.7549489
TACGGGA8750.035.742864
TAAGGGA33500.035.7313424
Produced by FastQC (version 0.11.5)