FastQCFastQC Report
Thu 26 May 2016
SRR1545209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545209_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2919771
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120880.4140050709456324No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79810.27334335466719817No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41200.14110695667571188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG351.212793E-745.01
ATAGCGC253.892288E-545.036
CTACGTC207.0350745E-445.032
TCGTAAT207.0350745E-445.036
ATACGTA406.8212103E-945.044
CAATCGA253.892288E-545.029
TATAGCG2000.042.751
CGGGATC3500.041.7857136
TACGGGA4750.041.6842084
CTAAGCG650.041.5384641
AATACGG2500.040.5000042
ACCCGCT11850.040.4430434
CGGGTAC1800.040.06
CGTACTC451.9301297E-840.028
ATAGGGC12800.039.550784
AATCGTC403.4606637E-739.37520
TTACGAG1950.039.230771
AATGCGG3050.039.0983622
GGGCGAT44500.039.033717
TAGAACG750.039.0000041
Produced by FastQC (version 0.11.5)