##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545209_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2919771
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.198296030750356	31.0	31.0	33.0	30.0	34.0
2	31.452000173986246	31.0	31.0	34.0	30.0	34.0
3	31.547314498294558	31.0	31.0	34.0	30.0	34.0
4	35.30892456976934	37.0	35.0	37.0	33.0	37.0
5	35.16853342265541	37.0	35.0	37.0	32.0	37.0
6	35.15532040012727	37.0	35.0	37.0	32.0	37.0
7	35.812516461051224	37.0	35.0	37.0	35.0	37.0
8	35.89607164397482	37.0	35.0	37.0	35.0	37.0
9	37.54586609703295	39.0	37.0	39.0	35.0	39.0
10	36.74941219705244	39.0	37.0	39.0	32.0	39.0
11	36.40035639781339	38.0	35.0	39.0	32.0	39.0
12	35.4419230138254	37.0	35.0	39.0	30.0	39.0
13	34.98826620306867	37.0	34.0	39.0	29.0	39.0
14	35.74554271550748	38.0	34.0	40.0	27.0	41.0
15	36.19780695129858	38.0	35.0	40.0	30.0	41.0
16	36.12649930422626	37.0	35.0	40.0	31.0	41.0
17	36.18532549299243	37.0	34.0	40.0	31.0	41.0
18	36.27416910435784	37.0	35.0	40.0	31.0	41.0
19	36.20325189886467	37.0	35.0	40.0	31.0	41.0
20	36.04429354219903	36.0	34.0	40.0	31.0	41.0
21	35.91692773166115	36.0	34.0	40.0	30.0	41.0
22	35.78133661852248	36.0	34.0	40.0	30.0	41.0
23	35.71531705739937	36.0	34.0	40.0	30.0	41.0
24	35.544043008852405	36.0	34.0	40.0	30.0	41.0
25	35.51257204760236	36.0	34.0	40.0	30.0	41.0
26	35.374028990629746	36.0	34.0	40.0	29.0	41.0
27	35.323861700112786	36.0	34.0	40.0	29.0	41.0
28	35.368521709408036	36.0	34.0	40.0	29.0	41.0
29	35.36335692079961	36.0	34.0	40.0	29.0	41.0
30	35.27386805335076	36.0	34.0	40.0	29.0	41.0
31	35.143773604162796	36.0	34.0	40.0	29.0	41.0
32	34.98645236218868	35.0	34.0	40.0	28.0	41.0
33	34.80203173468056	35.0	34.0	40.0	27.0	41.0
34	34.628368800155904	35.0	34.0	40.0	26.0	41.0
35	34.45617515894226	35.0	33.0	40.0	25.0	41.0
36	34.24052228753556	35.0	33.0	40.0	24.0	41.0
37	34.04455383658513	35.0	33.0	40.0	23.0	41.0
38	34.10634566888979	35.0	33.0	40.0	24.0	41.0
39	34.0928247455023	35.0	33.0	40.0	24.0	41.0
40	33.92031498360659	35.0	33.0	40.0	23.0	41.0
41	33.94693316701892	35.0	33.0	40.0	23.0	41.0
42	33.87986523600652	35.0	33.0	39.0	23.0	41.0
43	33.7398172664911	35.0	33.0	39.0	23.0	41.0
44	33.715871210447666	35.0	33.0	39.0	23.0	41.0
45	33.66116931773074	35.0	33.0	39.0	23.0	41.0
46	33.57987561353271	35.0	33.0	39.0	23.0	41.0
47	33.58008213657852	35.0	33.0	39.0	23.0	41.0
48	33.57849536830114	35.0	33.0	39.0	23.0	41.0
49	33.56158068560856	35.0	33.0	39.0	24.0	41.0
50	33.3301885661581	35.0	32.0	39.0	23.0	40.0
51	33.13573838496238	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	5.0
12	10.0
13	8.0
14	37.0
15	106.0
16	332.0
17	868.0
18	1874.0
19	3556.0
20	6266.0
21	10062.0
22	15087.0
23	21721.0
24	29937.0
25	39953.0
26	51528.0
27	61262.0
28	68296.0
29	79015.0
30	94754.0
31	115925.0
32	144148.0
33	188233.0
34	311328.0
35	441803.0
36	206990.0
37	247571.0
38	330388.0
39	448389.0
40	316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.10373621766912	24.291494093201145	27.533563419870944	14.071206269258788
2	32.05497965422631	26.192465094009087	28.738794926040434	13.013760325724174
3	29.46196807900346	26.288979512434363	30.610140315798738	13.638912092763439
4	26.368574795763095	28.127103118703488	30.30378067321033	15.20054141232309
5	23.580890419145888	32.481759699647675	29.23619694832232	14.70115293288412
6	21.15597421852604	41.333104548267656	27.129867376585352	10.381053856620948
7	85.94684309146162	5.192016771178288	6.882252067028545	1.9788880703315432
8	87.41274572560657	4.459219575781799	6.385295285143938	1.7427394134677001
9	83.69115249106865	5.250857002141606	7.848046987246603	3.2099435195431423
10	53.08450559992547	22.464638493909284	13.540616712749049	10.910239193416196
11	46.63711640399196	20.958835470315996	19.76240602430807	12.64164210138398
12	42.20594697323865	21.224369993400167	23.326795149345617	13.24288788401556
13	21.569362802767753	41.21124567645887	22.86806054310424	14.351330977669138
14	15.373876923909444	42.81808402097288	27.247034099592053	14.561004955525622
15	14.22036180234683	22.69876644435471	48.33755797971827	14.743313773580189
16	16.341452805716614	18.056587314553095	45.94634305224622	19.655616827484074
17	15.932482376186352	19.179312350180886	29.765724777730856	35.122480495901904
18	20.214838766464904	23.12058034688337	36.08450799737376	20.58007288927796
19	30.947598287673934	23.237130583186147	25.817983670637183	19.99728745850274
20	32.301505837272856	22.67051080375824	25.733422244415742	19.29456111455316
21	22.393845270742123	29.963925253042106	28.037575549589334	19.604653926626437
22	21.460689896570656	25.372400780746162	24.012362613369337	29.154546709313845
23	19.609722817303137	31.4117442772053	25.236876453667083	23.741656451824475
24	18.410621928911546	23.507939492514996	41.2887175055852	16.792721072988257
25	17.78755936681336	24.653337539142626	38.214298313121134	19.344804780922885
26	16.150410426023136	35.28828116999587	27.598363022305517	20.96294538167548
27	16.931464830632265	37.57130268092943	27.41814340919202	18.079089079246284
28	15.871963931417909	29.82196206483317	37.369780027269265	16.936293976479664
29	16.291859875312138	25.669958363173002	36.52272729607905	21.515454465435816
30	18.643242911858497	33.742029768772966	29.478373475180074	18.136353844188466
31	29.506457869469898	27.999832863604713	24.39256366338319	18.101145603542196
32	32.25321437879888	25.00935861065817	26.37987705200168	16.35754995854127
33	30.919787887474737	26.37665762143675	23.345837738644573	19.35771675244394
34	20.49331951033146	26.263566560528208	26.98454776076617	26.258566168374166
35	20.821735677215784	24.355437464102494	32.18738729852444	22.635439560157288
36	32.06182950649212	26.406385980270368	23.99153906248127	17.54024545075624
37	20.671107425890593	35.99162400064937	26.20825400348178	17.129014569978263
38	20.42177280341506	36.090056377709075	23.01296916778747	20.47520165108839
39	21.252385889167336	34.13510854104654	25.029668422626294	19.58283714715983
40	25.639716265419445	24.721801812539407	25.926314084220987	23.712167837820157
41	17.506304432779146	24.369753655338037	27.514452332049327	30.609489579833486
42	19.48375403413487	25.77630231959972	24.885650278737614	29.854293367527795
43	20.182815707122238	25.272290189881332	26.92495404605361	27.61994005694282
44	18.25920594457579	29.09211030591098	30.715525292908247	21.933158456604986
45	17.30399404610841	38.377907034490036	23.921567821586006	20.396531097815547
46	20.96883625462408	35.08710100894899	24.723308780037886	19.220753956389043
47	20.953047345151383	27.975241893970455	26.293226420839165	24.778484340038997
48	20.916606131097268	25.005385696343996	32.93624739748425	21.141760775074484
49	21.392876359139123	25.848157269868082	33.83840719015293	18.920559180839867
50	19.87604507339788	35.51244258539454	25.389285666581387	19.222226674626196
51	17.94205778466873	34.682171992255554	25.317327968529042	22.058442254546677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9031.0
1	8513.5
2	7996.0
3	15600.5
4	23205.0
5	17353.5
6	11502.0
7	11714.0
8	11926.0
9	12632.0
10	13338.0
11	14141.0
12	14944.0
13	14527.5
14	14111.0
15	13624.0
16	13137.0
17	12677.0
18	12217.0
19	12164.0
20	12111.0
21	12640.0
22	13169.0
23	11964.5
24	10760.0
25	12866.0
26	19944.0
27	24916.0
28	29011.0
29	33106.0
30	40750.5
31	48395.0
32	54881.5
33	61368.0
34	72720.0
35	84072.0
36	90249.5
37	96427.0
38	108798.0
39	121169.0
40	167611.5
41	214054.0
42	261943.5
43	309833.0
44	315025.5
45	320218.0
46	288998.0
47	257778.0
48	237243.5
49	216709.0
50	199133.0
51	181557.0
52	164162.0
53	146767.0
54	127429.5
55	108092.0
56	101568.5
57	95045.0
58	91934.0
59	88823.0
60	86496.0
61	84169.0
62	77462.5
63	70756.0
64	62402.5
65	54049.0
66	44442.5
67	34836.0
68	29959.0
69	25082.0
70	23470.0
71	21858.0
72	18977.5
73	16097.0
74	13233.0
75	8293.5
76	6218.0
77	4493.0
78	2768.0
79	2167.0
80	1566.0
81	1065.0
82	564.0
83	477.0
84	390.0
85	245.5
86	101.0
87	122.5
88	144.0
89	86.5
90	29.0
91	19.0
92	9.0
93	9.0
94	9.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2919771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.714480354570174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94699704153605	13.195580311030639
2	7.681332216952342	2.5677895287435373
3	2.790696617882767	1.3993513138550078
4	1.4932038093827142	0.998325029491869
5	0.9282298486615832	0.7757439784989839
6	0.6401536989703356	0.6419901855207062
7	0.48020345576848716	0.5618445859347361
8	0.38094330838673596	0.5093815555388053
9	0.27819260690661535	0.41848603766345566
>10	3.6198372236153387	14.8172982619347
>50	1.478382961761054	17.79005805462802
>100	1.251090492509052	41.756503922705186
>500	0.026497170402547336	2.906893968239887
>1k	0.003815592537966816	0.9496902384659688
>5k	2.1197736322037868E-4	0.28277413047294797
>10k+	2.1197736322037868E-4	0.42828889727565395
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12088	0.4140050709456324	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7981	0.27334335466719817	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4120	0.14110695667571188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025549948951475988	0.0
2	0.0	0.0	0.0	0.07668409611575702	0.0
3	0.0	0.0	0.0	0.11425553579373177	0.0
4	0.0	0.0	0.0	0.16874611056826033	0.0
5	3.4249261329056285E-5	0.0	0.0	0.324340504786163	0.0
6	3.4249261329056285E-5	0.0	0.0	0.4804143886626725	0.0
7	3.4249261329056285E-5	0.0	0.0	0.5971358712720963	0.0
8	3.4249261329056285E-5	0.0	0.0	0.8818157314392122	0.0
9	3.4249261329056285E-5	0.0	0.0	1.0112779392630449	0.0
10	3.4249261329056285E-5	0.0	0.0	1.2502007862945417	0.0
11	3.4249261329056285E-5	0.0	0.0	1.4313793787252493	0.0
12	3.4249261329056285E-5	0.0	0.0	1.6138936923477902	0.0
13	6.849852265811257E-5	0.0	0.0	1.6871528623306418	0.0
14	6.849852265811257E-5	0.0	0.0	1.7120863245781948	0.0
15	1.0274778398716886E-4	0.0	0.0	1.7538361741383142	0.0
16	1.0274778398716886E-4	0.0	0.0	1.8373358732585534	0.0
17	1.0274778398716886E-4	0.0	0.0	1.9378917045206627	0.0
18	1.0274778398716886E-4	0.0	0.0	2.101705921457539	0.0
19	1.0274778398716886E-4	0.0	0.0	2.169279714059767	0.0
20	1.0274778398716886E-4	0.0	0.0	2.2467172939247635	0.0
21	1.0274778398716886E-4	0.0	0.0	2.342683724168779	0.0
22	1.0274778398716886E-4	0.0	0.0	2.4490276805954987	0.0
23	1.0274778398716886E-4	0.0	0.0	2.5700303208710547	0.0
24	2.054955679743377E-4	0.0	0.0	2.6641815402646305	0.0
25	2.054955679743377E-4	0.0	0.0	2.7389819270072895	0.0
26	2.054955679743377E-4	0.0	0.0	2.8171044920988666	0.0
27	2.39744829303394E-4	0.0	0.0	2.8932406000333586	0.0
28	2.739940906324503E-4	0.0	0.0	2.977356785857521	0.0
29	3.082433519615066E-4	0.0	0.0	3.0719874949097035	0.0
30	3.082433519615066E-4	0.0	0.0	3.1997029904057546	0.0
31	3.082433519615066E-4	0.0	0.0	3.3127940513142984	0.0
32	3.082433519615066E-4	0.0	0.0	3.411671668771284	0.0
33	3.082433519615066E-4	0.0	0.0	3.518734859685914	0.0
34	3.082433519615066E-4	0.0	0.0	3.6288119855975007	0.0
35	3.082433519615066E-4	0.0	0.0	3.776494800448391	0.0
36	3.082433519615066E-4	0.0	0.0	3.892976538228512	0.0
37	3.082433519615066E-4	0.0	0.0	4.013499688845461	0.0
38	3.082433519615066E-4	0.0	0.0	4.12857720691109	0.0
39	3.082433519615066E-4	0.0	0.0	4.257936666950935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	35	1.212793E-7	45.0	1
ATAGCGC	25	3.892288E-5	45.0	36
CTACGTC	20	7.0350745E-4	45.0	32
TCGTAAT	20	7.0350745E-4	45.0	36
ATACGTA	40	6.8212103E-9	45.0	44
CAATCGA	25	3.892288E-5	45.0	29
TATAGCG	200	0.0	42.75	1
CGGGATC	350	0.0	41.785713	6
TACGGGA	475	0.0	41.684208	4
CTAAGCG	65	0.0	41.538464	1
AATACGG	250	0.0	40.500004	2
ACCCGCT	1185	0.0	40.44304	34
CGGGTAC	180	0.0	40.0	6
CGTACTC	45	1.9301297E-8	40.0	28
ATAGGGC	1280	0.0	39.55078	4
AATCGTC	40	3.4606637E-7	39.375	20
TTACGAG	195	0.0	39.23077	1
AATGCGG	305	0.0	39.098362	2
GGGCGAT	4450	0.0	39.03371	7
TAGAACG	75	0.0	39.000004	1
>>END_MODULE