##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545208_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3570194
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223135493477386	31.0	31.0	33.0	30.0	34.0
2	31.479117381296366	31.0	31.0	34.0	30.0	34.0
3	31.563499910649114	31.0	31.0	34.0	30.0	34.0
4	35.32981344991337	37.0	35.0	37.0	33.0	37.0
5	35.17199457508472	37.0	35.0	37.0	32.0	37.0
6	35.158982397035004	37.0	35.0	37.0	32.0	37.0
7	35.82118674783499	37.0	35.0	37.0	35.0	37.0
8	35.9183450535181	37.0	35.0	37.0	35.0	37.0
9	37.54966732900229	39.0	37.0	39.0	35.0	39.0
10	36.77945848320847	39.0	37.0	39.0	32.0	39.0
11	36.43118357153701	38.0	35.0	39.0	32.0	39.0
12	35.448546773648715	37.0	35.0	39.0	30.0	39.0
13	35.001485353456985	37.0	34.0	39.0	29.0	39.0
14	35.77386999137862	38.0	34.0	40.0	28.0	41.0
15	36.23288286294807	38.0	35.0	40.0	30.0	41.0
16	36.15970784780883	38.0	35.0	40.0	31.0	41.0
17	36.253396874231484	37.0	35.0	40.0	31.0	41.0
18	36.32753374186389	37.0	35.0	40.0	31.0	41.0
19	36.23627399519466	37.0	35.0	40.0	31.0	41.0
20	36.08462481310539	36.0	34.0	40.0	31.0	41.0
21	35.93793978702558	36.0	34.0	40.0	30.0	41.0
22	35.82122007935703	36.0	34.0	40.0	30.0	41.0
23	35.775502395668134	36.0	34.0	40.0	30.0	41.0
24	35.60452401186042	36.0	34.0	40.0	30.0	41.0
25	35.56855201706126	36.0	34.0	40.0	30.0	41.0
26	35.423534127277115	36.0	34.0	40.0	29.0	41.0
27	35.36031599403282	36.0	34.0	40.0	29.0	41.0
28	35.39856125465451	36.0	34.0	40.0	29.0	41.0
29	35.40834055516311	36.0	34.0	40.0	29.0	41.0
30	35.309751514903674	36.0	34.0	40.0	29.0	41.0
31	35.18555154145685	36.0	34.0	40.0	29.0	41.0
32	35.0434365751553	35.0	34.0	40.0	29.0	41.0
33	34.85781584978295	35.0	34.0	40.0	27.0	41.0
34	34.710558025698326	35.0	34.0	40.0	27.0	41.0
35	34.533944934084815	35.0	33.0	40.0	26.0	41.0
36	34.29265132370958	35.0	33.0	40.0	24.0	41.0
37	34.10935568207218	35.0	33.0	40.0	24.0	41.0
38	34.17454233579464	35.0	33.0	40.0	24.0	41.0
39	34.17085458101156	35.0	33.0	40.0	24.0	41.0
40	34.00241583510588	35.0	33.0	40.0	23.0	41.0
41	34.02138259153424	35.0	33.0	40.0	23.0	41.0
42	33.93626228714742	35.0	33.0	40.0	23.0	41.0
43	33.810410582730235	35.0	33.0	39.0	23.0	41.0
44	33.781016661839665	35.0	33.0	39.0	23.0	41.0
45	33.69367154838084	35.0	33.0	39.0	23.0	41.0
46	33.61151718926198	35.0	33.0	39.0	23.0	41.0
47	33.639438921246295	35.0	33.0	39.0	23.0	41.0
48	33.60260926997245	35.0	33.0	39.0	23.0	41.0
49	33.586508464245924	35.0	33.0	39.0	24.0	41.0
50	33.359074044715776	35.0	32.0	39.0	23.0	40.0
51	33.16573497126487	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	11.0
13	15.0
14	22.0
15	117.0
16	360.0
17	976.0
18	2238.0
19	4297.0
20	7429.0
21	11983.0
22	17795.0
23	25040.0
24	35246.0
25	47430.0
26	61998.0
27	73306.0
28	83294.0
29	96693.0
30	115442.0
31	140842.0
32	174623.0
33	228293.0
34	379849.0
35	549734.0
36	253133.0
37	296669.0
38	397679.0
39	565220.0
40	455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.018821946370416	24.166669934462945	28.33719960315882	14.477308516007813
2	32.00100610779134	26.297254434913057	29.004138150475857	12.697601306819742
3	28.100965941906797	26.747790176108076	31.64505906401725	13.506184817967876
4	26.42579086738704	28.258828511839972	30.895547973023312	14.419832647749676
5	23.526144517636858	32.895383276090875	29.291293414307457	14.287178791964806
6	20.747051840880356	41.8614786759487	26.737174506483402	10.654294976687542
7	86.09759021498552	5.468666408604126	6.738849485490145	1.6948938909202134
8	87.66181893757033	4.240693923075329	6.4012487836795415	1.6962383556747895
9	83.556103673918	5.726355486564596	7.932566129459631	2.7849747100577726
10	52.849481008595056	23.19086861946438	13.442294732443111	10.517355639497461
11	46.811293728015904	21.01488042386492	19.144057717871917	13.029768130247263
12	41.66389837639075	22.110731237574203	23.403462108781763	12.82190827725328
13	20.655124063286195	42.39080565369837	23.150982831745278	13.803087451270155
14	14.374428952600335	44.5081975937442	26.31069908245882	14.806674371196635
15	14.222672493427527	24.131769870208732	47.90168265365972	13.743874982704021
16	15.640298538398753	19.097001451461743	46.50027421479057	18.762425795348936
17	15.382049266790542	19.292172918334412	29.181607498079938	36.14417031679511
18	19.57148547109765	23.94326470774417	37.09703170191872	19.388218119239458
19	31.062905825285686	23.521775007184484	25.54830353756687	19.867015629962967
20	32.514143489121324	22.886683468741474	25.55919930401541	19.039973738121795
21	21.4241018835391	30.85294524611268	27.777762216843115	19.945190653505104
22	21.38656890914051	25.41018779371653	23.57616981038005	29.627073486762907
23	18.59235100389503	31.83709344646257	25.312826137739293	24.257729411903107
24	17.23603815366896	24.416964456273245	42.054157281088926	16.292840108968868
25	17.053050898634638	25.902093835797157	38.331978598361886	18.71287666720632
26	15.927285744136032	36.645067466921965	26.860641186445335	20.56700560249667
27	16.681894597324405	38.59157233472467	27.767510673089475	16.959022394861456
28	15.200686573334671	30.579178610462066	37.9492823079082	16.270852508295068
29	16.29368039944048	26.04499923533567	35.70531461315547	21.956005752068375
30	17.90283665257406	35.08604854526113	28.97948962997529	18.03162517218952
31	30.337959225745152	29.201858498445745	23.65246818520226	16.80771409060684
32	31.97582540332542	25.43214738470795	26.162611891678715	16.42941532028792
33	30.198723094599337	27.639030259980267	23.039336237750664	19.122910407669725
34	20.123920436816597	27.95229054779656	25.72599696263004	26.197792052756796
35	21.486675513991678	25.396883194582703	31.33011819525774	21.786323096167884
36	32.13027079200738	27.412095813280736	23.268455439676387	17.189177955035497
37	20.287665040051046	37.287637590562305	26.555727783980366	15.868969585406283
38	19.99034226151296	36.90922678151383	23.29416832810766	19.806262628865547
39	21.267387710583794	35.20909507998725	25.020965247266673	18.50255196216228
40	25.630595984419895	25.441334560530883	26.399349727213707	22.52871972783552
41	17.341662665950366	25.491443882321242	27.151045573433823	30.015847878294572
42	19.239402676717287	26.34377851735788	25.124012868768475	29.29280593715636
43	20.120279178106287	25.536791558105804	27.2668656101041	27.076063653683807
44	18.044453606722772	30.32017307742941	30.43072729381092	21.204646022036897
45	17.113775890049673	40.43931506243078	22.972729213034363	19.474179834485184
46	20.652042998223628	36.76057939708598	24.363073827360644	18.224303777329748
47	20.9664236733354	29.01074843551919	25.59468757159975	24.428140319545662
48	20.603950373565134	27.31504226380975	32.410367615877455	19.670639746747657
49	21.541742549564532	26.66950311383639	33.37981073297417	18.4089436036249
50	19.779625420915504	36.63845718187863	25.247871684283822	18.33404571292204
51	18.37440766524172	35.02720020256602	25.205100899278865	21.393291232913395
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13093.0
1	12240.5
2	11388.0
3	21777.0
4	32166.0
5	23359.0
6	14552.0
7	14849.0
8	15146.0
9	16361.0
10	17576.0
11	18630.0
12	19684.0
13	19384.5
14	19085.0
15	19001.5
16	18918.0
17	17697.5
18	16477.0
19	15738.5
20	15000.0
21	14114.0
22	13228.0
23	15462.0
24	17696.0
25	21411.5
26	29316.0
27	33505.0
28	39007.5
29	44510.0
30	58800.0
31	73090.0
32	83234.0
33	93378.0
34	94483.0
35	95588.0
36	108848.5
37	122109.0
38	140250.0
39	158391.0
40	216832.5
41	275274.0
42	326306.0
43	377338.0
44	378293.0
45	379248.0
46	348712.5
47	318177.0
48	290994.5
49	263812.0
50	233663.0
51	203514.0
52	186448.0
53	169382.0
54	150615.5
55	131849.0
56	125097.5
57	118346.0
58	112588.0
59	106830.0
60	98855.0
61	90880.0
62	84141.5
63	77403.0
64	66992.0
65	56581.0
66	47796.0
67	39011.0
68	34118.5
69	29226.0
70	26133.5
71	23041.0
72	20023.0
73	17005.0
74	14063.0
75	8580.5
76	6040.0
77	4595.5
78	3151.0
79	2426.0
80	1701.0
81	1204.5
82	708.0
83	566.0
84	424.0
85	339.5
86	255.0
87	149.0
88	43.0
89	80.0
90	117.0
91	63.5
92	10.0
93	5.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3570194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.725473321999345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76056346744085	12.385471396328775
2	8.203225929858258	2.5799922102863864
3	2.926414304702535	1.3805775023315094
4	1.5653041274061312	0.9846059318536233
5	0.9971132198824453	0.784003866913713
6	0.688164421983364	0.6493026755429095
7	0.518389206435658	0.5706340945351446
8	0.3828088746566119	0.4815880596669713
9	0.3305307173366307	0.46779767798206434
>10	3.022720980651181	11.011564110246788
>50	1.1417033126424454	13.166784383513292
>100	1.3969521113141705	46.56389923939969
>500	0.05708604725282966	5.795439419237864
>1k	0.00847083281816182	2.1353798400777495
>5k	1.8414853952525696E-4	0.15000362858153976
>10k+	3.682970790505139E-4	0.892955963502
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19127	0.5357411950162932	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11709	0.3279653710694713	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5180	0.14509015476469905	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4667	0.1307211877001642	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4167	0.11671634650666043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01366872500485968	0.0
2	0.0	0.0	0.0	0.05338645462963637	0.0
3	0.0	0.0	0.0	0.08862263507249186	0.0
4	0.0	0.0	0.0	0.12828434533249453	0.0
5	0.0	0.0	0.0	0.2481937956312738	0.0
6	0.0	0.0	0.0	0.37446144383190383	0.0
7	0.0	0.0	0.0	0.46524082444819526	0.0
8	0.0	0.0	0.0	0.6681709733420649	0.0
9	0.0	0.0	0.0	0.7729551951518601	0.0
10	0.0	0.0	0.0	0.9549901209850221	0.0
11	0.0	0.0	0.0	1.1504136749991738	0.0
12	0.0	0.0	0.0	1.3152226461643262	0.0
13	0.0	0.0	0.0	1.3807092835851498	0.0
14	0.0	0.0	0.0	1.4056098912271995	0.0
15	0.0	0.0	0.0	1.4433949527672727	0.0
16	0.0	0.0	0.0	1.5274520096106823	0.0
17	0.0	0.0	0.0	1.6254858979652087	0.0
18	2.8009682387007542E-5	0.0	0.0	1.779371092999428	0.0
19	2.8009682387007542E-5	0.0	0.0	1.8455019531151529	0.0
20	2.8009682387007542E-5	0.0	0.0	1.916926643202022	0.0
21	2.8009682387007542E-5	0.0	0.0	2.0063335493813503	0.0
22	2.8009682387007542E-5	0.0	0.0	2.1033310794875573	0.0
23	2.8009682387007542E-5	0.0	0.0	2.221531939160729	0.0
24	5.6019364774015084E-5	0.0	0.0	2.313263648978179	0.0
25	5.6019364774015084E-5	0.0	0.0	2.3869291136560085	0.0
26	5.6019364774015084E-5	0.0	0.0	2.4576535616832027	0.0
27	5.6019364774015084E-5	0.0	0.0	2.529106261452459	0.0
28	5.6019364774015084E-5	0.0	0.0	2.6056287137337635	0.0
29	5.6019364774015084E-5	0.0	0.0	2.692570767863035	0.0
30	8.402904716102262E-5	0.0	0.0	2.8129003633976195	0.0
31	1.1203872954803017E-4	0.0	0.0	2.922558269942754	0.0
32	1.6805809432204525E-4	0.0	0.0	3.0203960905205713	0.0
33	1.6805809432204525E-4	0.0	0.0	3.1226034215507616	0.0
34	1.6805809432204525E-4	0.0	0.0	3.2210574551410933	0.0
35	1.6805809432204525E-4	0.0	0.0	3.361750089771032	0.0
36	1.6805809432204525E-4	0.0	0.0	3.476701826287311	0.0
37	2.2407745909606034E-4	0.0	0.0	3.5863037134676716	0.0
38	2.2407745909606034E-4	0.0	0.0	3.6927405065383003	0.0
39	2.5208714148306784E-4	0.0	0.0	3.807244088136387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	55	1.8189894E-12	45.0	1
TATACGA	55	1.8189894E-12	45.0	1
TTCGCAT	25	3.8924634E-5	45.0	1
CGACGGT	340	0.0	43.014706	28
CTCGCTA	75	0.0	42.0	14
GTATGCG	215	0.0	41.860466	1
TAATACG	70	0.0	41.785713	1
TCTAGCG	135	0.0	41.666668	1
TACGGGA	720	0.0	41.562504	4
CAACCGT	65	0.0	41.53846	28
AGTACGG	245	0.0	41.32653	2
ACGTATA	60	3.6379788E-12	41.249996	13
TCGTAAG	285	0.0	41.05263	1
ATGGGAC	1665	0.0	40.81081	5
CGTAAGG	580	0.0	40.732758	2
AGGGATC	2020	0.0	40.65594	6
CGGTCTA	385	0.0	40.324677	31
GCGTTAG	280	0.0	40.17857	1
TAACGCA	45	1.9303116E-8	40.000004	25
CGTAGGT	45	1.9303116E-8	40.000004	24
>>END_MODULE