FastQCFastQC Report
Thu 26 May 2016
SRR1545207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545207_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences439106
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28240.643124894672357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13860.3156413257846625No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5560.1266209070247275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA207.0285203E-445.05
ATCGTAA253.8868544E-545.014
AGTGCGT207.0285203E-445.024
AAGCACG253.8868544E-545.01
GTCGTTT253.8868544E-545.09
GCGATAT406.7993824E-945.09
CGTACAT253.8868544E-545.035
GTGCGAG351.209628E-745.01
TGCGTAT207.0285203E-445.026
TGCGTAG253.8868544E-545.01
TACGGGA1100.042.9545444
ATTGCGG700.041.7857132
CGTTTTT19650.041.335881
GACCGAT1200.041.2499969
TGGGCGA2050.040.6097566
CACGGGA1000.040.54
ACGGGCT1500.040.55
CACCCAT1400.040.1785731
TCGTCCC1850.040.13513638
ACGCTAG451.9235813E-840.01