##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545207_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	439106
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.119845777557128	31.0	31.0	33.0	28.0	34.0
2	31.392155880356906	31.0	31.0	34.0	30.0	34.0
3	31.475434177624535	31.0	31.0	34.0	30.0	34.0
4	35.26637531712161	37.0	35.0	37.0	33.0	37.0
5	35.13243954762631	37.0	35.0	37.0	32.0	37.0
6	35.11568505098997	37.0	35.0	37.0	32.0	37.0
7	35.77444398391277	37.0	35.0	37.0	35.0	37.0
8	35.863306354274364	37.0	35.0	37.0	35.0	37.0
9	37.48087933209749	39.0	37.0	39.0	35.0	39.0
10	36.664472815220016	39.0	35.0	39.0	32.0	39.0
11	36.36880161054506	38.0	35.0	39.0	32.0	39.0
12	35.7060094829039	37.0	35.0	39.0	31.0	39.0
13	35.411000988371825	37.0	35.0	39.0	30.0	39.0
14	36.24074369286687	38.0	35.0	40.0	30.0	41.0
15	36.574674452182386	38.0	35.0	40.0	31.0	41.0
16	36.446195223932264	38.0	35.0	40.0	31.0	41.0
17	36.48659776910359	38.0	35.0	40.0	31.0	41.0
18	36.49277167699827	38.0	35.0	40.0	31.0	41.0
19	36.41731381488752	38.0	35.0	40.0	31.0	41.0
20	36.31440699967661	38.0	35.0	40.0	31.0	41.0
21	36.187521919536515	38.0	34.0	40.0	30.0	41.0
22	36.09520935719394	38.0	34.0	40.0	30.0	41.0
23	36.01551561581942	37.0	34.0	40.0	30.0	41.0
24	35.81359170678606	37.0	34.0	40.0	30.0	41.0
25	35.76969114519046	37.0	34.0	40.0	30.0	41.0
26	35.615083829417046	37.0	34.0	40.0	29.0	41.0
27	35.59001698906415	37.0	34.0	40.0	29.0	41.0
28	35.5735972635309	37.0	34.0	40.0	29.0	41.0
29	35.531286295336436	37.0	34.0	40.0	29.0	41.0
30	35.40517323835247	37.0	34.0	40.0	29.0	41.0
31	35.280572344718585	37.0	34.0	40.0	28.0	41.0
32	35.14841974375208	37.0	34.0	40.0	27.0	41.0
33	34.96093198453221	37.0	34.0	40.0	27.0	41.0
34	34.76852058500681	37.0	34.0	40.0	25.0	41.0
35	34.58342404795197	36.0	33.0	40.0	25.0	41.0
36	34.41664199532687	36.0	33.0	40.0	24.0	41.0
37	34.21929784607817	36.0	33.0	40.0	23.0	41.0
38	34.243244228045164	36.0	33.0	40.0	24.0	41.0
39	34.22868282373732	36.0	33.0	40.0	23.0	41.0
40	34.09532322491608	36.0	33.0	40.0	23.0	41.0
41	34.04155716387387	36.0	33.0	40.0	23.0	41.0
42	33.972835716205196	35.0	33.0	40.0	23.0	41.0
43	33.829262182707595	35.0	33.0	40.0	23.0	41.0
44	33.74673085769723	35.0	33.0	40.0	23.0	41.0
45	33.67404681329793	35.0	33.0	40.0	23.0	41.0
46	33.582626973896964	35.0	32.0	39.0	23.0	41.0
47	33.58861186137288	35.0	32.0	39.0	23.0	41.0
48	33.55784935755831	35.0	32.0	39.0	23.0	41.0
49	33.517444535032546	35.0	32.0	39.0	23.0	41.0
50	33.33310407965275	35.0	32.0	39.0	23.0	41.0
51	33.16505126324851	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	8.0
15	18.0
16	42.0
17	135.0
18	299.0
19	603.0
20	1011.0
21	1540.0
22	2317.0
23	3419.0
24	4605.0
25	6384.0
26	8007.0
27	9324.0
28	10450.0
29	11797.0
30	13926.0
31	17171.0
32	20793.0
33	26211.0
34	41316.0
35	54325.0
36	34566.0
37	41766.0
38	55645.0
39	73386.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.26961599249384	23.400955577924236	25.96411800339781	15.365310426184111
2	33.88771731654771	25.021065528596743	28.063838799743113	13.027378355112432
3	28.48719899067651	26.22578602888596	31.749509230117557	13.537505750319967
4	26.136058263836066	27.993013076569213	30.761137401902953	15.109791257691766
5	22.6635482093162	32.6451927325065	29.531593738186224	15.159665319991072
6	20.95940387970103	40.790606368393966	28.24921545139442	10.000774300510583
7	85.58070260939272	4.848487608914477	7.700874048635181	1.8699357330576216
8	86.69956684718497	4.0145204119278715	7.550113184515812	1.7357995563713546
9	82.39217865390134	5.404162092979827	9.042008080053563	3.161651173065273
10	46.500844898498315	25.511607675595414	15.987028189093294	12.000519236812979
11	38.56130410424818	23.8792000109313	22.65694388143182	14.902552003388703
12	34.766548396059264	23.057302792492017	26.983917322924306	15.19223148852441
13	21.95233041680141	35.25071395061784	26.60473780818299	16.192217824397755
14	17.36163933082217	36.938916799132784	29.90280251237742	15.796641357667623
15	16.58597240757357	24.560128989355647	42.63253064180403	16.221367961266754
16	18.764945138531473	20.99811890523017	40.87555168911379	19.361384267124567
17	18.79728357162052	20.908618875624565	30.477379038318762	29.816718514436154
18	21.439697931706693	23.857337408279548	35.053267320419216	19.64969733959454
19	29.02078313664582	24.975973910627502	26.83748343224643	19.16575952048025
20	30.29245785755603	24.313036032301998	27.243080258525275	18.151425851616693
21	23.41985761980023	28.227808319631254	28.789631660692404	19.562702399876112
22	22.187808866196317	25.02630344381539	26.32621735981745	26.459670330170848
23	20.03775853666313	29.570991970048233	27.431189735508056	22.96005975778058
24	19.80319102904538	24.560356724799934	37.41442840680838	18.222023839346306
25	19.897473502981057	26.6546118704823	33.795939932499216	19.65197469403743
26	18.710288631902092	32.75951592553962	27.70401679776637	20.826178644791916
27	18.501227494044716	32.76224875087109	28.990266587111087	19.74625716797311
28	17.87632143491549	28.803978993682616	34.60918320405551	18.71051636734638
29	18.527644805582252	25.759383838981933	34.6178371509385	21.09513420449732
30	20.780631555934104	29.866137105846878	30.939226519337016	18.414004818882002
31	27.831776382012546	27.5136299663407	26.46855201249812	18.186041639148634
32	29.467372342896702	25.58972093298657	26.635254357717724	18.307652366399
33	28.06998765673892	27.034930062445056	24.947962450979947	19.947119829836076
34	20.691814732661364	27.042445332106595	27.648904820248415	24.616835114983626
35	20.96851329747259	27.12329141482922	30.7937035704363	21.114491717261892
36	28.515665921212648	27.794200033704847	24.9864497410648	18.70368430401771
37	21.30009610435749	33.872459041780346	26.70539687455876	18.122047979303403
38	20.99151457734579	33.67136864447309	25.29730862251939	20.03980815566173
39	20.75193688995368	32.56730721055964	25.9456714324104	20.735084467076287
40	24.826807194618155	25.8308927684887	27.59789208072766	21.74440795616548
41	18.759251752424245	26.06955951410366	28.088661963170626	27.082526770301474
42	20.656743474240844	27.496549808019022	25.244929470332906	26.60177724740723
43	20.725064107527565	26.80742235360027	27.428912381065164	25.038601157807
44	19.981963352812304	28.84929834709615	30.322746671646485	20.845991628445066
45	18.91115129376506	35.351828487882194	25.0782271251133	20.658793093239446
46	21.13977035157798	33.07629592854573	26.04382540889899	19.740108310977302
47	20.576808333295375	29.47830364422258	27.157223996028296	22.78766402645375
48	20.809098486470237	27.25241741174113	30.99228887785637	20.946195223932264
49	20.94642295937655	27.621348831489435	31.542497711258783	19.889730497875227
50	19.781328426393628	33.82235724403675	26.991888063474423	19.404426266095204
51	18.77519323352448	32.84218389181655	26.900338414870212	21.482284459788755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1525.0
1	1338.5
2	1152.0
3	2985.0
4	4818.0
5	3446.0
6	2074.0
7	2066.5
8	2059.0
9	2229.5
10	2400.0
11	2442.5
12	2485.0
13	2465.0
14	2445.0
15	2388.0
16	2331.0
17	2363.0
18	2395.0
19	2322.0
20	2249.0
21	2301.0
22	2353.0
23	2451.0
24	2549.0
25	2733.5
26	3682.5
27	4447.0
28	5176.0
29	5905.0
30	7229.5
31	8554.0
32	9406.5
33	10259.0
34	12716.5
35	15174.0
36	16299.5
37	17425.0
38	19688.0
39	21951.0
40	26058.0
41	30165.0
42	34526.0
43	38887.0
44	38102.5
45	37318.0
46	35217.5
47	33117.0
48	30619.0
49	28121.0
50	26711.5
51	25302.0
52	23289.5
53	21277.0
54	19328.0
55	17379.0
56	17043.5
57	16708.0
58	16381.5
59	16055.0
60	15188.5
61	14322.0
62	12887.5
63	11453.0
64	10334.0
65	9215.0
66	8020.5
67	6826.0
68	5874.5
69	4923.0
70	4419.0
71	3915.0
72	3338.5
73	2762.0
74	2301.5
75	1430.5
76	1020.0
77	809.0
78	598.0
79	404.0
80	210.0
81	172.0
82	134.0
83	102.0
84	70.0
85	39.5
86	9.0
87	7.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	439106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.677280627700448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84101977448347	22.440508633487898
2	7.099827361671338	4.498064474877781
3	3.7648342674750044	3.577791348227663
4	2.6443159575676383	3.3505895462470603
5	2.100568820756187	3.327015400644578
6	1.6921386302930521	3.216141015165941
7	1.4713377860339933	3.26255859624368
8	1.2699687840720768	3.2183326049176566
9	1.0707202163628173	3.05257542907294
>10	7.923744089207639	46.064796128847426
>50	0.10488705506627599	2.13238112560393
>100	0.014467180009141515	0.7671622618226905
>500	7.233590004570757E-4	0.1274020960494074
>1k	0.0014467180009141514	0.9646813387913761
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2824	0.643124894672357	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1386	0.3156413257846625	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	556	0.1266209070247275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014802803878790087	0.0
2	0.0	0.0	0.0	0.04987406229930814	0.0
3	0.0	0.0	0.0	0.07538043205968491	0.0
4	0.0	0.0	0.0	0.10566924615013232	0.0
5	0.0	0.0	0.0	0.18605985798417693	0.0
6	0.0	0.0	0.0	0.2949174003543564	0.0
7	0.0	0.0	0.0	0.38396195907138597	0.0
8	0.0	0.0	0.0	0.5745765259413445	0.0
9	0.0	0.0	0.0	0.6622546719926395	0.0
10	0.0	0.0	0.0	0.7758946586928896	0.0
11	2.2773544428907825E-4	0.0	0.0	0.8706326035171462	0.0
12	2.2773544428907825E-4	0.0	0.0	0.9594494267898868	0.0
13	2.2773544428907825E-4	0.0	0.0	1.0002140713176317	0.0
14	2.2773544428907825E-4	0.0	0.0	1.021165732192227	0.0
15	2.2773544428907825E-4	0.0	0.0	1.0407509804010877	0.0
16	2.2773544428907825E-4	0.0	0.0	1.0860703338146143	0.0
17	2.2773544428907825E-4	0.0	0.0	1.138221750556813	0.0
18	2.2773544428907825E-4	0.0	0.0	1.2236225421652176	0.0
19	2.2773544428907825E-4	0.0	0.0	1.2602879486957592	0.0
20	2.2773544428907825E-4	0.0	0.0	1.304013153999262	0.0
21	2.2773544428907825E-4	0.0	0.0	1.355709099852883	0.0
22	2.2773544428907825E-4	0.0	0.0	1.4160589925894886	0.0
23	2.2773544428907825E-4	0.0	0.0	1.4907562183163063	0.0
24	4.554708885781565E-4	0.0	0.0	1.550878375608623	0.0
25	4.554708885781565E-4	0.0	0.0	1.5991582897979075	0.0
26	4.554708885781565E-4	0.0	0.0	1.641061611547098	0.0
27	4.554708885781565E-4	0.0	0.0	1.6859254940720463	0.0
28	4.554708885781565E-4	0.0	0.0	1.7433148260328941	0.0
29	4.554708885781565E-4	0.0	0.0	1.8143682846510865	0.0
30	4.554708885781565E-4	0.0	0.0	1.9070566104767415	0.0
31	4.554708885781565E-4	0.0	0.0	1.9762881855406211	0.0
32	4.554708885781565E-4	0.0	0.0	2.045519760604501	0.0
33	4.554708885781565E-4	0.0	0.0	2.11247398122549	0.0
34	4.554708885781565E-4	0.0	0.0	2.1878544132851747	0.0
35	4.554708885781565E-4	0.0	0.0	2.2823646226651424	0.0
36	6.832063328672348E-4	0.0	0.0	2.365260324386367	0.0
37	6.832063328672348E-4	0.0	0.0	2.447472819774724	0.0
38	6.832063328672348E-4	0.0	0.0	2.545854531707606	0.0
39	6.832063328672348E-4	0.0	0.0	2.6722477032880443	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	20	7.0285203E-4	45.0	5
ATCGTAA	25	3.8868544E-5	45.0	14
AGTGCGT	20	7.0285203E-4	45.0	24
AAGCACG	25	3.8868544E-5	45.0	1
GTCGTTT	25	3.8868544E-5	45.0	9
GCGATAT	40	6.7993824E-9	45.0	9
CGTACAT	25	3.8868544E-5	45.0	35
GTGCGAG	35	1.209628E-7	45.0	1
TGCGTAT	20	7.0285203E-4	45.0	26
TGCGTAG	25	3.8868544E-5	45.0	1
TACGGGA	110	0.0	42.954544	4
ATTGCGG	70	0.0	41.785713	2
CGTTTTT	1965	0.0	41.33588	1
GACCGAT	120	0.0	41.249996	9
TGGGCGA	205	0.0	40.609756	6
CACGGGA	100	0.0	40.5	4
ACGGGCT	150	0.0	40.5	5
CACCCAT	140	0.0	40.17857	31
TCGTCCC	185	0.0	40.135136	38
ACGCTAG	45	1.9235813E-8	40.0	1
>>END_MODULE