FastQCFastQC Report
Thu 26 May 2016
SRR1545206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545206_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences466633
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18170.3893852342204687No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14420.30902229375119206No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6380.13672414938506278No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6190.13265242706795274No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6050.12965221062376645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA207.0289726E-445.00000412
ACGTTAG207.0289726E-445.0000041
CGTTGAT1150.045.00000425
TACGGGA650.045.0000044
TCGATAA207.0289726E-445.00000416
TCGGATT207.0289726E-445.00000414
GTTCGAC207.0289726E-445.00000413
GGAACGA207.0289726E-445.0000048
ACGTATA406.7993824E-945.00000413
CGCCGCA207.0289726E-445.00000418
AGTACGG650.045.0000042
ATCGCCA207.0289726E-445.00000424
TACACGT207.0289726E-445.0000041
CGACGGT551.8189894E-1245.028
TAGCGAG502.1827873E-1145.01
CGACAGG551.8189894E-1245.02
TCGATGG351.2098462E-745.02
GCGTTAG351.2098462E-745.01
ACGGGAA253.887231E-545.05
TGCGAAG351.2098462E-745.01