##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545206_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 466633 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19074733248613 31.0 31.0 33.0 30.0 34.0 2 31.45341199615115 31.0 31.0 34.0 30.0 34.0 3 31.554474287073567 31.0 31.0 34.0 30.0 34.0 4 35.31267827178961 37.0 35.0 37.0 33.0 37.0 5 35.15779852689373 37.0 35.0 37.0 32.0 37.0 6 35.15902004358886 37.0 35.0 37.0 32.0 37.0 7 35.81147711370606 37.0 35.0 37.0 35.0 37.0 8 35.90060925823934 37.0 35.0 37.0 35.0 37.0 9 37.55313061870892 39.0 37.0 39.0 35.0 39.0 10 36.74962122267392 39.0 37.0 39.0 32.0 39.0 11 36.337612213452545 38.0 35.0 39.0 32.0 39.0 12 35.43134326119242 37.0 35.0 39.0 30.0 39.0 13 35.03359599513965 37.0 34.0 39.0 30.0 39.0 14 35.803614403610545 38.0 34.0 40.0 29.0 41.0 15 36.249519429615994 38.0 35.0 40.0 30.0 41.0 16 36.17087518456689 38.0 35.0 40.0 31.0 41.0 17 36.263513296316376 37.0 35.0 40.0 31.0 41.0 18 36.34552635582995 37.0 35.0 40.0 31.0 41.0 19 36.26182460306065 37.0 35.0 40.0 31.0 41.0 20 36.13015153236055 37.0 34.0 40.0 31.0 41.0 21 36.00102650262626 37.0 34.0 40.0 30.0 41.0 22 35.889654182194576 37.0 34.0 40.0 30.0 41.0 23 35.833181536668 36.0 34.0 40.0 30.0 41.0 24 35.636755223055374 36.0 34.0 40.0 30.0 41.0 25 35.611718416828644 36.0 34.0 40.0 30.0 41.0 26 35.484393516960864 36.0 34.0 40.0 29.0 41.0 27 35.428220893078716 36.0 34.0 40.0 29.0 41.0 28 35.45990746475282 36.0 34.0 40.0 29.0 41.0 29 35.474597810270595 36.0 34.0 40.0 29.0 41.0 30 35.381779685534454 36.0 34.0 40.0 29.0 41.0 31 35.26348543716368 36.0 34.0 40.0 29.0 41.0 32 35.14424826362473 36.0 34.0 40.0 29.0 41.0 33 34.95943278765111 36.0 34.0 40.0 27.0 41.0 34 34.83321796786768 36.0 34.0 40.0 27.0 41.0 35 34.66456294346949 35.0 33.0 40.0 26.0 41.0 36 34.423660564083555 35.0 33.0 40.0 24.0 41.0 37 34.2584707896784 35.0 33.0 40.0 24.0 41.0 38 34.33759721237032 35.0 33.0 40.0 25.0 41.0 39 34.31755576652316 35.0 33.0 40.0 25.0 41.0 40 34.170122987444095 35.0 33.0 40.0 24.0 41.0 41 34.18088519243174 35.0 33.0 40.0 24.0 41.0 42 34.116436257187125 35.0 33.0 40.0 24.0 41.0 43 33.993243083965346 35.0 33.0 40.0 24.0 41.0 44 33.96257658588227 35.0 33.0 40.0 24.0 41.0 45 33.920646418063015 35.0 33.0 39.0 23.0 41.0 46 33.84477094418955 35.0 33.0 39.0 23.0 41.0 47 33.85719612629197 35.0 33.0 39.0 23.0 41.0 48 33.83608746059537 35.0 33.0 39.0 23.0 41.0 49 33.78726322398973 35.0 33.0 39.0 24.0 41.0 50 33.598856060330064 35.0 33.0 39.0 24.0 41.0 51 33.406177874260926 35.0 32.0 39.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 2.0 14 4.0 15 15.0 16 52.0 17 118.0 18 260.0 19 590.0 20 976.0 21 1498.0 22 2382.0 23 3145.0 24 4357.0 25 6109.0 26 7887.0 27 9481.0 28 10493.0 29 12149.0 30 14614.0 31 18268.0 32 22532.0 33 29339.0 34 48978.0 35 70417.0 36 33206.0 37 38978.0 38 53707.0 39 77020.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.83072564520726 24.54927105455465 28.880940696435957 13.739062603802132 2 31.052025896153935 26.17624557200198 29.593063499581042 13.178665032263043 3 28.5462022617346 26.42054891102858 31.443982744469423 13.589266082767399 4 26.483982058705664 28.865082409516685 30.526773717246748 14.124161814530906 5 22.910724273679744 33.395409240238045 29.70835753150763 13.98550895457458 6 21.47940672862828 42.238332908302674 25.99944710296957 10.282813260099479 7 87.19593342091108 5.263236847801163 5.916855430284613 1.6239743010031438 8 88.87541172613167 4.026719070447225 5.692267799319808 1.4056014041012959 9 84.84590674041485 5.686053065256851 6.910569976834043 2.557470217494262 10 53.12676128777862 24.395831413551978 12.642054891102857 9.835352407566546 11 45.03903495895061 21.375470658954683 20.82578814614483 12.75970623594988 12 41.204329740931314 22.27875010982935 23.598202441747585 12.918717707491755 13 20.64577515949365 41.632074885402446 22.85222005301811 14.869929902085794 14 14.783566528728143 43.24790574177137 26.494482816260316 15.47404491324017 15 13.887573317789354 24.730998450602506 47.30912730132674 14.072300930281397 16 15.271101700908424 20.018729922658707 45.584645749443354 19.125522626989518 17 14.927576918049088 20.69249281555312 29.89608536044386 34.48384490595393 18 20.06780489163861 23.99294520533267 36.191610966219706 19.74763893680901 19 29.840367055051832 23.908081940197114 25.7701448461639 20.481406158587156 20 30.8278668675383 22.960656447357987 26.843150827309685 19.368325857794026 21 21.167169917258317 30.408050866526796 28.423193387522954 20.001585828691926 22 22.53912603694981 25.60491864055907 24.069879327008593 27.786075995482527 23 19.19024158171411 31.455983610246168 25.670066197632828 23.683708610406896 24 17.406184303296165 24.32082600244732 42.23983301652476 16.03315667773175 25 16.958723450763234 27.177889262011046 37.3501231160248 18.513264171200923 26 16.195382666892396 36.58378211570978 26.46705226591347 20.753782951484357 27 16.475260000900064 38.805656693804345 27.11038439201685 17.608698913278744 28 14.740920594985782 30.96180510165376 37.53527933086601 16.761994972494445 29 15.645485852907958 25.741214187594963 35.88794620183313 22.725353757663946 30 17.98651188407164 34.57513720632703 29.461053976036844 17.977296933564492 31 28.994734620140456 29.231323116882002 24.42390486742258 17.350037395554967 32 31.340903879494164 25.887581889836337 26.054093902488678 16.71742032818082 33 28.933873086558386 28.227750716301674 23.605917284032635 19.232458913107305 34 19.53933819511265 27.612492044068894 27.31118459260275 25.536985168215708 35 20.611272670385507 25.656565223633994 31.109244309768062 22.62291779621244 36 30.82379514522119 28.26739643359985 23.610846211048084 17.297962210130873 37 19.543624218604343 36.852730089813626 27.561916966866896 16.04172872471514 38 19.38611285528456 36.15153664657236 24.69499585327227 19.767354644870807 39 21.34161107337029 34.6499283162571 25.77635958022686 18.23210103014575 40 24.81243289694471 26.548058109906503 26.23925011732989 22.4002588758189 41 16.921006444036323 25.75514376394297 27.837508277382867 29.486341514637843 42 19.668561803387245 25.94780051989465 25.30425409261668 29.079383584101425 43 19.607914570979766 26.68863968043409 27.576060844389488 26.12738490419666 44 17.55833813725133 30.279470161775958 30.57627728857582 21.585914412396896 45 16.56783810832067 40.012172306716415 23.46919313464757 19.950796450315345 46 20.221244532641283 36.23039947881954 24.676565952257985 18.87179003628119 47 20.476905833920874 28.945016747636792 26.83629318972297 23.741784228719357 48 20.77435586424449 26.403190515887214 32.87251437425128 19.949939245617003 49 21.051232981807974 27.80943482351227 32.57613584980059 18.563196344879167 50 19.25667494583538 36.58892534389981 25.71699815486689 18.437401555397926 51 17.755709519043876 35.175609097513465 25.5862744383702 21.482406945072466 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1752.0 1 1507.5 2 1263.0 3 2295.0 4 3327.0 5 2511.5 6 1696.0 7 1731.5 8 1767.0 9 1911.0 10 2055.0 11 2130.5 12 2206.0 13 2130.0 14 2054.0 15 1953.5 16 1853.0 17 1851.0 18 1849.0 19 1825.5 20 1802.0 21 1810.0 22 1818.0 23 2061.0 24 2304.0 25 2843.0 26 4205.0 27 5028.0 28 5866.5 29 6705.0 30 8361.5 31 10018.0 32 11287.5 33 12557.0 34 14166.5 35 15776.0 36 17135.5 37 18495.0 38 20534.5 39 22574.0 40 30210.0 41 37846.0 42 44596.0 43 51346.0 44 50771.5 45 50197.0 46 45948.5 47 41700.0 48 37829.5 49 33959.0 50 29796.5 51 25634.0 52 23642.0 53 21650.0 54 18633.0 55 15616.0 56 14308.5 57 13001.0 58 12439.0 59 11877.0 60 11715.5 61 11554.0 62 10181.0 63 8808.0 64 7573.0 65 6338.0 66 5519.0 67 4700.0 68 4075.5 69 3451.0 70 3229.5 71 3008.0 72 2598.0 73 2188.0 74 1867.5 75 1258.0 76 969.0 77 750.5 78 532.0 79 375.5 80 219.0 81 163.5 82 108.0 83 88.5 84 69.0 85 44.5 86 20.0 87 12.5 88 5.0 89 4.0 90 3.0 91 5.0 92 7.0 93 3.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 466633.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.981315608864097 #Duplication Level Percentage of deduplicated Percentage of total 1 74.7861686655431 20.17829219942761 2 6.101663173298457 3.292617996354978 3 2.756374330925479 2.231118172766159 4 1.8194421528995381 1.9636377183781444 5 1.4914229101173218 2.0120276122083007 6 1.1922188548019157 1.9300579917749405 7 1.0300053415231463 1.94536294389163 8 0.9307437658001589 2.0090153036829426 9 0.7815550200191441 1.897864439474576 >10 8.327269699883958 47.12877639454515 >50 0.7180731074923579 12.067458973085014 >100 0.06109572295791332 2.2474486286447357 >500 0.0023803528425161034 0.3986234850958752 >1k 0.0015869018950107356 0.6976981406699556 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1817 0.3893852342204687 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1442 0.30902229375119206 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 638 0.13672414938506278 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 619 0.13265242706795274 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 605 0.12965221062376645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.015643985744685868 0.0 2 0.0 0.0 0.0 0.05036077602741341 0.0 3 0.0 0.0 0.0 0.08336315691346305 0.0 4 0.0 0.0 0.0 0.11357962252991109 0.0 5 0.0 0.0 0.0 0.21387257223556844 0.0 6 0.0 0.0 0.0 0.32252326775003054 0.0 7 0.0 0.0 0.0 0.3973143776801041 0.0 8 0.0 0.0 0.0 0.5691839196970638 0.0 9 0.0 0.0 0.0 0.6429035237542137 0.0 10 0.0 0.0 0.0 0.7731986379017344 0.0 11 0.0 0.0 0.0 0.9069225708426107 0.0 12 0.0 0.0 0.0 1.0239310121658778 0.0 13 2.143011745847379E-4 0.0 0.0 1.0712915717491047 0.0 14 2.143011745847379E-4 0.0 0.0 1.0905786774617312 0.0 15 2.143011745847379E-4 0.0 0.0 1.1222952513002724 0.0 16 2.143011745847379E-4 0.0 0.0 1.1792993637398126 0.0 17 2.143011745847379E-4 0.0 0.0 1.2513045584002846 0.0 18 2.143011745847379E-4 0.0 0.0 1.358883748041823 0.0 19 2.143011745847379E-4 0.0 0.0 1.4034583923554484 0.0 20 2.143011745847379E-4 0.0 0.0 1.4581051918745567 0.0 21 2.143011745847379E-4 0.0 0.0 1.5286102783129354 0.0 22 2.143011745847379E-4 0.0 0.0 1.6019012800209158 0.0 23 2.143011745847379E-4 0.0 0.0 1.6938364839177684 0.0 24 2.143011745847379E-4 0.0 0.0 1.7662702809274098 0.0 25 2.143011745847379E-4 0.0 0.0 1.824131598065289 0.0 26 2.143011745847379E-4 0.0 0.0 1.8841359269490157 0.0 27 2.143011745847379E-4 0.0 0.0 1.947569074626098 0.0 28 2.143011745847379E-4 0.0 0.0 2.01550254696946 0.0 29 2.143011745847379E-4 0.0 0.0 2.0902936568995334 0.0 30 2.143011745847379E-4 0.0 0.0 2.1856576795897418 0.0 31 2.143011745847379E-4 0.0 0.0 2.2808074011053656 0.0 32 2.143011745847379E-4 0.0 0.0 2.360741739225473 0.0 33 2.143011745847379E-4 0.0 0.0 2.4432476914405967 0.0 34 2.143011745847379E-4 0.0 0.0 2.5351828953374493 0.0 35 2.143011745847379E-4 0.0 0.0 2.6536914448828095 0.0 36 2.143011745847379E-4 0.0 0.0 2.749912672271357 0.0 37 4.286023491694758E-4 0.0 0.0 2.8457052973107344 0.0 38 4.286023491694758E-4 0.0 0.0 2.9517843787301796 0.0 39 4.286023491694758E-4 0.0 0.0 3.064506796561752 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 20 7.0289726E-4 45.000004 12 ACGTTAG 20 7.0289726E-4 45.000004 1 CGTTGAT 115 0.0 45.000004 25 TACGGGA 65 0.0 45.000004 4 TCGATAA 20 7.0289726E-4 45.000004 16 TCGGATT 20 7.0289726E-4 45.000004 14 GTTCGAC 20 7.0289726E-4 45.000004 13 GGAACGA 20 7.0289726E-4 45.000004 8 ACGTATA 40 6.7993824E-9 45.000004 13 CGCCGCA 20 7.0289726E-4 45.000004 18 AGTACGG 65 0.0 45.000004 2 ATCGCCA 20 7.0289726E-4 45.000004 24 TACACGT 20 7.0289726E-4 45.000004 1 CGACGGT 55 1.8189894E-12 45.0 28 TAGCGAG 50 2.1827873E-11 45.0 1 CGACAGG 55 1.8189894E-12 45.0 2 TCGATGG 35 1.2098462E-7 45.0 2 GCGTTAG 35 1.2098462E-7 45.0 1 ACGGGAA 25 3.887231E-5 45.0 5 TGCGAAG 35 1.2098462E-7 45.0 1 >>END_MODULE