FastQCFastQC Report
Thu 26 May 2016
SRR1545205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545205_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences130427
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6290.4822621083057955No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.4017573048525229No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2640.2024120772539428No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.13110782276675842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC207.010276E-445.00000421
TATCAGG207.010276E-445.0000042
GGAATTA207.010276E-445.0000048
CGAAAAA207.010276E-445.00000422
TGTATAG207.010276E-445.0000041
TACGGGA406.7357178E-945.0000044
CCAGAAA406.7357178E-945.0000043
GTTACGG406.7357178E-945.0000042
AACGGGA207.010276E-445.0000044
CTACGAA207.010276E-445.00000411
TACGAAA207.010276E-445.00000420
GATATAG207.010276E-445.0000041
CATACGA207.010276E-445.00000418
TGCTATG207.010276E-445.0000041
CGAGACA207.010276E-445.00000422
GCTACGA207.010276E-445.00000410
TAGTGAG207.010276E-445.0000041
TACAGCG207.010276E-445.0000041
ACATACG207.010276E-445.00000417
CCCATTA207.010276E-445.00000433