##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545205_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	130427
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231332469504014	31.0	31.0	33.0	30.0	34.0
2	31.480352994395332	31.0	31.0	34.0	30.0	34.0
3	31.56525872710405	31.0	31.0	34.0	30.0	34.0
4	35.33542901393116	37.0	35.0	37.0	33.0	37.0
5	35.18406464919074	37.0	35.0	37.0	32.0	37.0
6	35.18026175561808	37.0	35.0	37.0	32.0	37.0
7	35.84027080282457	37.0	35.0	37.0	35.0	37.0
8	35.93747460265129	37.0	35.0	37.0	35.0	37.0
9	37.61222752957593	39.0	37.0	39.0	35.0	39.0
10	36.82132533907856	39.0	37.0	39.0	32.0	39.0
11	36.41284396635666	38.0	35.0	39.0	32.0	39.0
12	35.26647856655447	37.0	35.0	39.0	30.0	39.0
13	34.706310809878325	37.0	33.0	39.0	27.0	39.0
14	35.45988944006992	37.0	33.0	40.0	27.0	41.0
15	35.97270503806727	37.0	34.0	40.0	30.0	41.0
16	35.940012420741105	36.0	34.0	40.0	31.0	41.0
17	36.03744623429198	36.0	34.0	40.0	31.0	41.0
18	36.09338557200579	36.0	35.0	40.0	31.0	41.0
19	36.007145759696996	36.0	35.0	40.0	31.0	41.0
20	35.85068275740453	36.0	34.0	40.0	31.0	41.0
21	35.70155719291251	36.0	34.0	40.0	30.0	41.0
22	35.59127327930567	35.0	34.0	40.0	30.0	41.0
23	35.52970627247426	35.0	34.0	40.0	30.0	41.0
24	35.352112676056336	35.0	34.0	40.0	29.0	41.0
25	35.31935105461292	35.0	34.0	40.0	29.0	41.0
26	35.21698728024106	35.0	34.0	40.0	29.0	41.0
27	35.15897015188573	35.0	34.0	40.0	29.0	41.0
28	35.23774218528372	36.0	34.0	40.0	29.0	41.0
29	35.25641929968488	36.0	34.0	40.0	29.0	41.0
30	35.17093853266578	36.0	34.0	40.0	29.0	41.0
31	34.995116041923836	35.0	34.0	40.0	29.0	41.0
32	34.83317871299654	35.0	34.0	40.0	29.0	41.0
33	34.64121692594325	35.0	33.0	40.0	27.0	41.0
34	34.53111702331572	35.0	34.0	40.0	27.0	41.0
35	34.3468146932767	35.0	33.0	40.0	26.0	41.0
36	34.092181833516065	35.0	33.0	40.0	24.0	41.0
37	33.91620600029135	35.0	33.0	39.0	24.0	41.0
38	34.00239980985532	35.0	33.0	39.0	24.0	41.0
39	34.00007667124138	35.0	33.0	39.0	24.0	41.0
40	33.80570740720863	35.0	33.0	39.0	23.0	41.0
41	33.847891924218146	35.0	33.0	39.0	23.0	41.0
42	33.78177064564852	35.0	33.0	39.0	23.0	41.0
43	33.65173622026114	35.0	33.0	39.0	23.0	41.0
44	33.60876965658951	35.0	33.0	39.0	23.0	41.0
45	33.51881128907358	35.0	33.0	39.0	23.0	41.0
46	33.48065967936087	35.0	33.0	39.0	23.0	41.0
47	33.479846964202196	35.0	33.0	39.0	23.0	41.0
48	33.49496653300314	35.0	33.0	39.0	23.0	41.0
49	33.50314735445882	35.0	33.0	38.0	24.0	40.0
50	33.26745229132005	35.0	32.0	38.0	24.0	40.0
51	33.03625783005052	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	15.0
17	35.0
18	96.0
19	154.0
20	270.0
21	433.0
22	607.0
23	911.0
24	1315.0
25	1756.0
26	2262.0
27	2749.0
28	3075.0
29	3619.0
30	4326.0
31	5255.0
32	6658.0
33	8791.0
34	14883.0
35	22232.0
36	8756.0
37	10327.0
38	13553.0
39	18327.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.77618897927576	24.25878077391951	28.803851963167137	14.161178283637591
2	30.793470677083732	26.2859683961143	29.423355593550415	13.49720533325155
3	28.26101957416792	26.17019482162436	31.84386668404548	13.724918920162235
4	25.654964079523413	28.384460272796275	30.541222292930144	15.419353354750168
5	22.1219532765455	32.865894331695124	30.60102586120972	14.411126530549655
6	20.729603533010803	42.0526424743343	27.108650816165365	10.109103176489532
7	87.15296679368537	5.457458961718049	5.850015717604483	1.5395585269921106
8	89.13798523311891	3.8994993367937623	5.5609651375865425	1.4015502925007859
9	85.74604951428768	5.021199598242696	6.899645012152392	2.3331058753172274
10	58.019428492566725	21.850537082045896	11.37034509725747	8.759689328129912
11	50.433575870026914	20.045696059864905	17.88126691559263	11.639461154515553
12	44.62802947242519	21.664992677896446	21.065423570273026	12.641554279405337
13	20.291044032293925	45.8869712559516	21.168929746141522	12.653054965612947
14	14.257017335367678	46.9250998642919	25.570625714000933	13.247257086339484
15	13.148351184954036	22.572013463469986	51.53074133423294	12.748894017343035
16	15.11266838921389	18.28915791975588	48.85184816027356	17.746325530756668
17	14.92559056023676	17.54007989143352	28.370659449347148	39.16367009898257
18	19.83024987157567	22.280662746210524	38.1278416278838	19.76124575433001
19	32.46107017718724	22.95230282073497	24.642903693253697	19.943723308824094
20	34.307313669715626	21.854370644115097	25.06996250776296	18.76835317840631
21	21.57835417513245	31.746494207487714	27.000544365813827	19.67460725156601
22	21.864337905495027	24.76634439188205	22.687787037959932	30.681530664662994
23	17.665820727303398	33.17794628412828	23.9835310173507	25.172701971217613
24	18.020808574911637	22.857230481418725	43.26404808820259	15.857912855467044
25	16.236668787904346	24.861416731198293	40.150428975595545	18.751485505301815
26	15.653967353385417	37.667047467165546	26.82880078511351	19.85018439433553
27	15.704570372698903	39.96105100937689	26.96680901960484	17.367569598319367
28	13.94189853327915	30.106496354282474	40.12052719145576	15.83107792098262
29	14.768414515399419	25.105231278799632	39.13836858932583	20.987985616475115
30	17.724090870755287	34.985087443550796	29.895650440476278	17.39517124521763
31	32.5546090916758	27.53570963067463	23.400829582831776	16.508851694817793
32	34.1769725593627	24.474993674622585	26.11345810300015	15.23457566301456
33	32.336096053731204	25.816740398843795	22.70772156071979	19.139441986705208
34	20.218973065392902	26.831100922355034	25.572159138828614	27.377766873423447
35	19.812615486057336	24.62373588290768	33.13884395102241	22.424804680012574
36	33.51606645863203	25.515422420204402	23.931394573209534	17.037116547954028
37	19.77274644053762	36.89266792918644	27.297262069970174	16.037323560305765
38	19.360255161891327	37.96913215821877	22.34199973931778	20.32861294057212
39	20.894446701986553	35.43668105530296	25.26547417329234	18.40339806941814
40	27.465172088601285	24.104671578737534	24.689673150497978	23.740483182163203
41	16.627692118963097	23.620109333190214	26.131092488518483	33.621106059328206
42	19.989726053654536	24.2058776173645	24.251113649781104	31.553282679199857
43	20.599262422657887	24.122305964255865	26.045220698168325	29.233210914917922
44	17.342268088662625	29.88721660392403	31.947372859913976	20.823142447499368
45	16.29953920583928	41.835662861217386	22.27682918414132	19.587968748802012
46	20.7464712061153	36.38433759881006	24.259547486333354	18.609643708741288
47	20.025761537105048	28.336157390724313	25.28157513398299	26.356505938187645
48	21.487882110299246	24.55779861531738	33.834252110375914	20.12006716400745
49	20.444386515062067	25.13206621328406	34.91761675113282	19.50593052052106
50	18.88566017772394	38.14624272581597	24.84608248292148	18.12201461353861
51	17.332300827282694	37.28369126024519	23.590974261464268	21.793033651007843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	704.0
1	547.0
2	390.0
3	668.5
4	947.0
5	702.5
6	458.0
7	482.0
8	506.0
9	540.5
10	575.0
11	645.0
12	715.0
13	712.0
14	709.0
15	640.0
16	571.0
17	551.5
18	532.0
19	538.0
20	544.0
21	535.5
22	527.0
23	585.0
24	643.0
25	685.5
26	860.5
27	993.0
28	1177.0
29	1361.0
30	1651.5
31	1942.0
32	2173.5
33	2405.0
34	2881.0
35	3357.0
36	3617.5
37	3878.0
38	4895.5
39	5913.0
40	8161.0
41	10409.0
42	12569.5
43	14730.0
44	14666.5
45	14603.0
46	13357.5
47	12112.0
48	10779.0
49	9446.0
50	8890.0
51	8334.0
52	7452.5
53	6571.0
54	5752.5
55	4934.0
56	4507.5
57	4081.0
58	3948.0
59	3815.0
60	3600.5
61	3386.0
62	2987.5
63	2589.0
64	2321.5
65	2054.0
66	1826.5
67	1599.0
68	1385.0
69	1171.0
70	1012.0
71	853.0
72	676.0
73	499.0
74	458.5
75	315.0
76	212.0
77	156.0
78	100.0
79	83.0
80	66.0
81	46.0
82	26.0
83	23.0
84	20.0
85	10.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	130427.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.42532604445398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.08268651355827	26.160994272658268
2	8.939082546840392	6.869743228012605
3	5.359458866252968	6.1781686307283
4	3.7452361474150484	5.75647680311592
5	2.8373605762515712	5.451325262407323
6	2.077139493585011	4.7888857368489655
7	1.4805355468204402	3.9823042774885566
8	1.1513059440908275	3.5391445022886368
9	0.8739549454276992	3.0223803353600096
>10	5.417323463096355	32.35219701442186
>50	0.02793463295887623	0.6808406234905349
>100	0.003990661851268033	0.33351990002070125
>500	0.003990661851268033	0.8840194131583184
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	629	0.4822621083057955	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.4017573048525229	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	264	0.2024120772539428	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	171	0.13110782276675842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0306684965536277	0.0
2	0.0	0.0	0.0	0.0927722020747238	0.0
3	0.0	0.0	0.0	0.13034111035291773	0.0
4	0.0	0.0	0.0	0.16791001863111166	0.0
5	0.0	0.0	0.0	0.3243193510546129	0.0
6	0.0	0.0	0.0	0.46309429795977824	0.0
7	0.0	0.0	0.0	0.5581666372760241	0.0
8	0.0	0.0	0.0	0.8019811848773644	0.0
9	0.0	0.0	0.0	0.9361558572994856	0.0
10	0.0	0.0	0.0	1.1408680717949504	0.0
11	0.0	0.0	0.0	1.3133783649091062	0.0
12	0.0	0.0	0.0	1.4475530373312275	0.0
13	0.0	0.0	0.0	1.5196240042322526	0.0
14	0.0	0.0	0.0	1.5472256511305174	0.0
15	0.0	0.0	0.0	1.5740605856149417	0.0
16	0.0	0.0	0.0	1.633097441480675	0.0
17	0.0	0.0	0.0	1.732770055279965	0.0
18	0.0	0.0	0.0	1.8991466490833953	0.0
19	0.0	0.0	0.0	1.9489829559830403	0.0
20	0.0	0.0	0.0	2.0064863870210923	0.0
21	7.667124138406925E-4	0.0	0.0	2.0731903670252323	0.0
22	7.667124138406925E-4	0.0	0.0	2.144494621512417	0.0
23	7.667124138406925E-4	0.0	0.0	2.2618016208300427	0.0
24	0.001533424827681385	0.0	0.0	2.350740260835563	0.0
25	0.001533424827681385	0.0	0.0	2.425878077391951	0.0
26	0.001533424827681385	0.0	0.0	2.49104863256841	0.0
27	0.001533424827681385	0.0	0.0	2.5700200111940013	0.0
28	0.001533424827681385	0.0	0.0	2.647457964991911	0.0
29	0.001533424827681385	0.0	0.0	2.7279627684451837	0.0
30	0.001533424827681385	0.0	0.0	2.8514034670735353	0.0
31	0.001533424827681385	0.0	0.0	2.93420840776833	0.0
32	0.001533424827681385	0.0	0.0	3.0331143091537793	0.0
33	0.001533424827681385	0.0	0.0	3.116685962262415	0.0
34	0.001533424827681385	0.0	0.0	3.21099158916482	0.0
35	0.001533424827681385	0.0	0.0	3.3474663988284634	0.0
36	0.001533424827681385	0.0	0.0	3.466306822973771	0.0
37	0.001533424827681385	0.0	0.0	3.5606124498761758	0.0
38	0.001533424827681385	0.0	0.0	3.6541513643647403	0.0
39	0.001533424827681385	0.0	0.0	3.7561241154055525	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	20	7.010276E-4	45.000004	21
TATCAGG	20	7.010276E-4	45.000004	2
GGAATTA	20	7.010276E-4	45.000004	8
CGAAAAA	20	7.010276E-4	45.000004	22
TGTATAG	20	7.010276E-4	45.000004	1
TACGGGA	40	6.7357178E-9	45.000004	4
CCAGAAA	40	6.7357178E-9	45.000004	3
GTTACGG	40	6.7357178E-9	45.000004	2
AACGGGA	20	7.010276E-4	45.000004	4
CTACGAA	20	7.010276E-4	45.000004	11
TACGAAA	20	7.010276E-4	45.000004	20
GATATAG	20	7.010276E-4	45.000004	1
CATACGA	20	7.010276E-4	45.000004	18
TGCTATG	20	7.010276E-4	45.000004	1
CGAGACA	20	7.010276E-4	45.000004	22
GCTACGA	20	7.010276E-4	45.000004	10
TAGTGAG	20	7.010276E-4	45.000004	1
TACAGCG	20	7.010276E-4	45.000004	1
ACATACG	20	7.010276E-4	45.000004	17
CCCATTA	20	7.010276E-4	45.000004	33
>>END_MODULE