FastQCFastQC Report
Thu 26 May 2016
SRR1545204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545204_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3685314
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223770.6071938510531261No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA158250.4294071007246601No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT72890.19778504626742796No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46970.12745182635726562No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG43760.11874157805820618No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39190.10634100649225549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAT551.8189894E-1245.00000427
ATACGTC302.166811E-645.00000410
TTACGCG1450.043.4482731
TAATACG2000.042.751
CGGTCTA2950.042.71186431
CTATACG1300.041.538461
GCGTTAG2550.041.470591
GCGATAC1900.041.447379
TCGATAG1250.041.3999981
ATCGTAG603.6379788E-1241.2500041
ACGTATA603.6379788E-1241.25000413
CGACGGT3250.040.15384728
CGTATGA2200.039.88636815
CGTTTTT138050.039.8496931
TAGTACG1300.039.807691
GCTACGA10100.039.65346510
GTCGAAT403.460991E-739.3759
ACGGGAT9500.039.315795
ACGGGTA3150.039.2857135
CGTTAGG4700.039.2553182
Produced by FastQC (version 0.11.5)