##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545204_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3685314 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27470902072388 31.0 31.0 33.0 30.0 34.0 2 31.532618930164432 31.0 31.0 34.0 30.0 34.0 3 31.625062613389254 31.0 31.0 34.0 30.0 34.0 4 35.37638068289432 37.0 35.0 37.0 33.0 37.0 5 35.238732439081176 37.0 35.0 37.0 33.0 37.0 6 35.234367003734285 37.0 35.0 37.0 32.0 37.0 7 35.85567959745085 37.0 35.0 37.0 35.0 37.0 8 35.942991831903605 37.0 35.0 37.0 35.0 37.0 9 37.576734302694426 39.0 37.0 39.0 35.0 39.0 10 36.825080033885854 39.0 37.0 39.0 32.0 39.0 11 36.49751418739353 39.0 35.0 39.0 32.0 39.0 12 35.63838576577193 37.0 35.0 39.0 31.0 39.0 13 35.219899308444276 37.0 35.0 39.0 30.0 39.0 14 36.022184541127295 38.0 35.0 40.0 30.0 41.0 15 36.44296903873049 38.0 35.0 40.0 31.0 41.0 16 36.35652158811976 38.0 35.0 40.0 31.0 41.0 17 36.41272548282182 38.0 35.0 40.0 31.0 41.0 18 36.49705262563787 38.0 35.0 40.0 31.0 41.0 19 36.41501673941488 37.0 35.0 40.0 31.0 41.0 20 36.26652491483765 37.0 35.0 40.0 31.0 41.0 21 36.130658337389974 37.0 34.0 40.0 31.0 41.0 22 36.02066499625269 37.0 34.0 40.0 30.0 41.0 23 35.957993267330814 37.0 34.0 40.0 30.0 41.0 24 35.76519259959938 36.0 34.0 40.0 30.0 41.0 25 35.7288174087744 36.0 34.0 40.0 30.0 41.0 26 35.624144374129315 36.0 34.0 40.0 30.0 41.0 27 35.56523758898156 36.0 34.0 40.0 30.0 41.0 28 35.61893803350271 36.0 34.0 40.0 30.0 41.0 29 35.60453546156447 36.0 34.0 40.0 30.0 41.0 30 35.51335300058557 36.0 34.0 40.0 30.0 41.0 31 35.40366872402189 36.0 34.0 40.0 29.0 41.0 32 35.27220068629159 36.0 34.0 40.0 29.0 41.0 33 35.085514558596635 36.0 34.0 40.0 28.0 41.0 34 34.93013431148608 36.0 34.0 40.0 27.0 41.0 35 34.772387644580625 36.0 34.0 40.0 27.0 41.0 36 34.53237119008041 35.0 33.0 40.0 25.0 41.0 37 34.32784343477923 35.0 33.0 40.0 24.0 41.0 38 34.401558184729986 35.0 33.0 40.0 25.0 41.0 39 34.39690484989882 35.0 33.0 40.0 25.0 41.0 40 34.214352426957376 35.0 33.0 40.0 24.0 41.0 41 34.23991171444278 35.0 33.0 40.0 24.0 41.0 42 34.170972134260474 35.0 33.0 40.0 24.0 41.0 43 34.05991131284878 35.0 33.0 40.0 24.0 41.0 44 34.002714829726855 35.0 33.0 40.0 24.0 41.0 45 33.94094180305939 35.0 33.0 39.0 23.0 41.0 46 33.843898783115904 35.0 33.0 39.0 23.0 41.0 47 33.87238699334711 35.0 33.0 39.0 23.0 41.0 48 33.83149685481346 35.0 33.0 39.0 23.0 41.0 49 33.82867294347239 35.0 33.0 39.0 24.0 41.0 50 33.608454801951744 35.0 33.0 39.0 24.0 41.0 51 33.4425441088602 35.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 3.0 12 4.0 13 6.0 14 29.0 15 116.0 16 328.0 17 947.0 18 2086.0 19 3991.0 20 7004.0 21 11109.0 22 16784.0 23 23947.0 24 33439.0 25 46384.0 26 59381.0 27 70306.0 28 80978.0 29 93853.0 30 112351.0 31 138460.0 32 175444.0 33 230286.0 34 382881.0 35 550323.0 36 270314.0 37 319117.0 38 430694.0 39 624264.0 40 484.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.681688995835906 23.89215681486028 28.315199193338746 14.110954995965066 2 30.86724767550336 26.690832857118824 29.3342439748689 13.107675492508916 3 28.888963057150622 26.324405464500444 31.528765255823522 13.257866222525408 4 26.507564891349826 28.345617225560698 30.3829741509136 14.763843732175875 5 22.57207933977946 33.24842876346493 29.598726187239404 14.580765709516205 6 21.29107587575984 41.598083636835284 26.934529866383162 10.17631062102171 7 86.09738546023486 5.486099691912276 6.53176364347787 1.8847512043749868 8 87.44967185971127 4.498666870719836 6.304944436213576 1.7467168333553125 9 82.84034956044451 6.41923591856759 7.8681490912307614 2.872265429757139 10 50.262392838167926 24.39062180318963 14.375762825094416 10.971222533548024 11 44.28135567281377 22.237019694929657 19.959031984791526 13.522592647465045 12 40.29634923916931 21.772120367491073 24.77387272834825 13.157657664991367 13 20.68323079118903 42.017043866547056 22.927788514085908 14.371936828178006 14 14.88993882203796 43.09722319455005 26.691538360096317 15.32129962331568 15 14.672291153481087 23.80508146659959 46.92628633543844 14.596341044480877 16 16.153657463108978 19.676016751896853 44.5024494520684 19.66787633292577 17 14.77591868698298 20.55575183010186 29.93937558644935 34.72895389646581 18 19.386489183825315 24.200570154944735 36.83707819740733 19.57586246382262 19 30.02889306040137 25.101524591934364 25.34410907727266 19.525473270391615 20 32.19535703063565 22.868336320867098 26.357265622413724 18.57904102608353 21 21.37896526591764 30.796588838834353 28.051910909084004 19.772534986164 22 20.985593086504974 26.14735135187938 23.442208723598586 29.42484683801706 23 18.780787742916885 31.924498156737798 25.099869373410243 24.194844726935074 24 17.712954717020043 25.199806583645245 41.35745285205006 15.729785847284655 25 16.792164792470874 27.667981615677796 36.63695956436819 18.90289402748314 26 17.11878553632065 35.84402848712484 26.692406671453234 20.344779305101273 27 16.375673823180332 37.674808713721546 27.443604534104825 18.505912928993297 28 15.536288088341998 30.659639857010827 36.51251969303023 17.291552361616947 29 15.452631716049162 25.76836057931563 35.982361340173455 22.796646364461754 30 17.90913881422316 34.23192704882135 30.381753088067935 17.47718104888756 31 28.657503811072814 29.703384840477636 24.630791297566503 17.008320050883047 32 30.554954069042694 26.349423685471578 26.14632023214304 16.94930201334269 33 28.91688469422144 28.569288804156173 23.262902428395517 19.25092407322687 34 18.984650968682722 27.692457142050852 27.046080741016915 26.276811148249514 35 20.069958760637494 25.924331006801594 31.863065128236016 22.142645104324895 36 30.606591460049266 28.28958400830974 23.39160245232835 17.712222079312646 37 19.60793571456869 37.04286798899633 26.700709898803737 16.648486397631245 38 19.59881844532108 36.008899106019186 25.039196117345767 19.353086331313968 39 20.59596549981901 35.38930468340011 25.25939445051358 18.755335366267296 40 25.289459731246783 26.209842634847398 26.836926242919873 21.66377139098595 41 16.983600311940855 26.326332030323602 27.527722196806025 29.16234546092952 42 19.288994099281634 26.973088317576195 24.811155847235813 28.92676173590636 43 19.77258925562381 26.225200891972843 26.666303061285955 27.33590679111739 44 17.433304190633418 29.900817135256318 30.97171638563227 21.694162288477997 45 16.922954190606283 39.970786749785766 23.1546077213502 19.951651338257744 46 19.821703116749344 36.579433936972535 24.889982237605803 18.708880708672314 47 20.04005628828371 30.036707862613603 26.1572826630241 23.76595318607858 48 19.951976955016587 26.603106275340444 32.76855106511955 20.676365704523413 49 20.85377256863323 27.106129898293606 33.26281017031384 18.77728736275932 50 18.999140914451253 36.251890612306035 26.432293150597207 18.316675322645505 51 17.786761182357864 35.33899689415881 25.596462065376247 21.27777985810707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17792.0 1 15122.5 2 12453.0 3 24774.0 4 37095.0 5 26433.0 6 15771.0 7 16082.5 8 16394.0 9 17637.5 10 18881.0 11 19263.0 12 19645.0 13 19642.0 14 19639.0 15 18632.5 16 17626.0 17 16887.5 18 16149.0 19 15839.5 20 15530.0 21 15688.0 22 15846.0 23 16334.0 24 16822.0 25 20005.5 26 29530.5 27 35872.0 28 48328.5 29 60785.0 30 68324.5 31 75864.0 32 88167.5 33 100471.0 34 114576.5 35 128682.0 36 129422.5 37 130163.0 38 150327.5 39 170492.0 40 220687.0 41 270882.0 42 333973.0 43 397064.0 44 391829.5 45 386595.0 46 351472.0 47 316349.0 48 292462.0 49 268575.0 50 234927.0 51 201279.0 52 182986.0 53 164693.0 54 143367.0 55 122041.0 56 116976.0 57 111911.0 58 106484.0 59 101057.0 60 99730.0 61 98403.0 62 87574.5 63 76746.0 64 68037.0 65 59328.0 66 50485.5 67 41643.0 68 36719.5 69 31796.0 70 28832.0 71 25868.0 72 22940.0 73 20012.0 74 15992.5 75 9559.5 76 7146.0 77 5557.0 78 3968.0 79 2792.5 80 1617.0 81 1242.0 82 867.0 83 540.0 84 213.0 85 148.5 86 84.0 87 55.0 88 26.0 89 18.5 90 11.0 91 8.0 92 5.0 93 3.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3685314.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.474949129604507 #Duplication Level Percentage of deduplicated Percentage of total 1 78.90108380380377 12.209902581345254 2 8.002730733206418 2.476837019885838 3 2.9434189499339416 1.3664777555202499 4 1.4667103832319528 0.907890742735088 5 0.9417518203383999 0.728678075622459 6 0.6408122577111176 0.5949922253823935 7 0.4719000006145289 0.5111839952639119 8 0.3652774381952455 0.45221198194109424 9 0.2997507728223246 0.41747651648865997 >10 3.1479041656759925 11.55722784920388 >50 1.3284241445211542 14.98095043755843 >100 1.4492900469560281 47.35131634131508 >500 0.034576185635258654 3.53331391823784 >1k 0.005823357580675142 1.63045482815574 >5k 1.819799243960982E-4 0.20526772099464896 >10k+ 3.639598487921964E-4 1.07581801034951 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22377 0.6071938510531261 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15825 0.4294071007246601 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 7289 0.19778504626742796 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 4697 0.12745182635726562 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 4376 0.11874157805820618 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3919 0.10634100649225549 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.7134729903612013E-5 0.0 0.0 0.0277859634212987 0.0 2 2.7134729903612013E-5 0.0 0.0 0.0772525760355834 0.0 3 2.7134729903612013E-5 0.0 0.0 0.1125005901803754 0.0 4 2.7134729903612013E-5 0.0 0.0 0.1599592327817928 0.0 5 2.7134729903612013E-5 0.0 0.0 0.29962168759568386 0.0 6 2.7134729903612013E-5 0.0 0.0 0.45762721982441656 0.0 7 2.7134729903612013E-5 0.0 0.0 0.5671972591752019 0.0 8 2.7134729903612013E-5 0.0 0.0 0.8192246305199503 0.0 9 2.7134729903612013E-5 0.0 0.0 0.941493723465626 0.0 10 5.4269459807224026E-5 0.0 0.0 1.1511366467009323 0.0 11 5.4269459807224026E-5 0.0 0.0 1.342924917659662 0.0 12 5.4269459807224026E-5 0.0 0.0 1.5114044556311892 0.0 13 5.4269459807224026E-5 0.0 0.0 1.5821446964899055 0.0 14 5.4269459807224026E-5 0.0 0.0 1.6113145311362884 0.0 15 5.4269459807224026E-5 0.0 0.0 1.6503342727376826 0.0 16 5.4269459807224026E-5 0.0 0.0 1.7337735671912895 0.0 17 5.4269459807224026E-5 0.0 0.0 1.8324625798507264 0.0 18 5.4269459807224026E-5 0.0 0.0 1.9858552079958451 0.0 19 5.4269459807224026E-5 0.0 0.0 2.0512227723336465 0.0 20 8.140418971083604E-5 0.0 0.0 2.124188061044459 0.0 21 8.140418971083604E-5 0.0 0.0 2.219539501925752 0.0 22 8.140418971083604E-5 0.0 0.0 2.3221087809614054 0.0 23 8.140418971083604E-5 0.0 0.0 2.4491807211000203 0.0 24 8.140418971083604E-5 0.0 0.0 2.5459431679363007 0.0 25 1.0853891961444805E-4 0.0 0.0 2.6243625373577393 0.0 26 1.0853891961444805E-4 0.0 0.0 2.7002855116280458 0.0 27 1.0853891961444805E-4 0.0 0.0 2.7801158870044724 0.0 28 1.0853891961444805E-4 0.0 0.0 2.863500911998272 0.0 29 1.0853891961444805E-4 0.0 0.0 2.960941727082143 0.0 30 1.3567364951806005E-4 0.0 0.0 3.092843649143601 0.0 31 1.3567364951806005E-4 0.0 0.0 3.202847844172844 0.0 32 1.3567364951806005E-4 0.0 0.0 3.303490557385341 0.0 33 1.3567364951806005E-4 0.0 0.0 3.4089903872505842 0.0 34 1.3567364951806005E-4 0.0 0.0 3.517854923623876 0.0 35 1.6280837942167208E-4 0.0 0.0 3.6714103601484163 0.0 36 1.6280837942167208E-4 0.0 0.0 3.792919680656791 0.0 37 1.6280837942167208E-4 0.0 0.0 3.9095176150526116 0.0 38 1.6280837942167208E-4 0.0 0.0 4.024297522544891 0.0 39 1.6280837942167208E-4 0.0 0.0 4.14238786708541 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTAAT 55 1.8189894E-12 45.000004 27 ATACGTC 30 2.166811E-6 45.000004 10 TTACGCG 145 0.0 43.448273 1 TAATACG 200 0.0 42.75 1 CGGTCTA 295 0.0 42.711864 31 CTATACG 130 0.0 41.53846 1 GCGTTAG 255 0.0 41.47059 1 GCGATAC 190 0.0 41.44737 9 TCGATAG 125 0.0 41.399998 1 ATCGTAG 60 3.6379788E-12 41.250004 1 ACGTATA 60 3.6379788E-12 41.250004 13 CGACGGT 325 0.0 40.153847 28 CGTATGA 220 0.0 39.886368 15 CGTTTTT 13805 0.0 39.849693 1 TAGTACG 130 0.0 39.80769 1 GCTACGA 1010 0.0 39.653465 10 GTCGAAT 40 3.460991E-7 39.375 9 ACGGGAT 950 0.0 39.31579 5 ACGGGTA 315 0.0 39.285713 5 CGTTAGG 470 0.0 39.255318 2 >>END_MODULE