FastQCFastQC Report
Thu 26 May 2016
SRR1545203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545203_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1916586
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.43629662326657925No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78020.40707800223939866No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41400.21600909116522818No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22540.11760494963440202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG302.1662636E-645.0000041
CTACGAT600.045.00000436
CTATCGA453.8562575E-1045.025
CGACTTA207.0344686E-445.017
ATCGCTA207.0344686E-445.041
TGCCGTA207.0344686E-445.031
CGTATGG1950.042.692312
TGTATAC700.041.78571313
CGTTTTT61950.040.5690081
GTAGGGC5850.040.3846134
TTCGTAC1750.039.85714333
GTCGTTT850.039.7058839
AGGGCGA12500.039.426
TCCGATT403.459827E-739.37540
GCGATAT1600.039.3759
TAATGCG800.039.3751
TATGGGA14050.038.914594
TAGCGGG12850.038.8715973
CGTTGAT3300.038.86363625
GGACTAA30600.038.823538
Produced by FastQC (version 0.11.5)