##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545203_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1916586
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229056770737134	31.0	31.0	33.0	30.0	34.0
2	31.485927581647783	31.0	31.0	34.0	30.0	34.0
3	31.568636627837208	31.0	31.0	34.0	30.0	34.0
4	35.32002738202199	37.0	35.0	37.0	33.0	37.0
5	35.1758668799626	37.0	35.0	37.0	32.0	37.0
6	35.16664057861218	37.0	35.0	37.0	32.0	37.0
7	35.82053766436779	37.0	35.0	37.0	35.0	37.0
8	35.93572738191764	37.0	35.0	37.0	35.0	37.0
9	37.60622638378867	39.0	37.0	39.0	35.0	39.0
10	36.83101201824494	39.0	37.0	39.0	32.0	39.0
11	36.43111762268951	38.0	35.0	39.0	32.0	39.0
12	35.22742470204833	37.0	35.0	39.0	30.0	39.0
13	34.64197327957107	35.0	33.0	39.0	27.0	39.0
14	35.401158100914856	37.0	33.0	40.0	27.0	41.0
15	35.94657218616853	37.0	34.0	40.0	30.0	41.0
16	35.93484351863157	36.0	34.0	40.0	31.0	41.0
17	36.01736212202322	36.0	34.0	40.0	31.0	41.0
18	36.10068215044877	36.0	35.0	40.0	31.0	41.0
19	35.996930479508876	36.0	35.0	40.0	31.0	41.0
20	35.826028156315445	36.0	34.0	40.0	31.0	41.0
21	35.65639475609235	35.0	34.0	40.0	30.0	41.0
22	35.532211964399195	35.0	34.0	40.0	30.0	41.0
23	35.502580108588916	35.0	34.0	40.0	30.0	41.0
24	35.34629440056434	35.0	34.0	40.0	30.0	41.0
25	35.308853868284544	35.0	34.0	40.0	30.0	41.0
26	35.15487434427675	35.0	34.0	40.0	29.0	41.0
27	35.106592660073694	35.0	34.0	40.0	29.0	41.0
28	35.18734823274301	36.0	34.0	40.0	29.0	41.0
29	35.20946359829405	36.0	34.0	40.0	29.0	41.0
30	35.127307097098694	36.0	34.0	40.0	29.0	41.0
31	34.9497669293212	35.0	34.0	40.0	29.0	41.0
32	34.78201656487108	35.0	34.0	40.0	29.0	41.0
33	34.59635675101457	35.0	33.0	40.0	27.0	41.0
34	34.44852774673299	35.0	34.0	40.0	27.0	41.0
35	34.26138717490371	35.0	33.0	40.0	25.0	41.0
36	34.00777163143214	35.0	33.0	39.0	24.0	41.0
37	33.81064820467227	35.0	33.0	39.0	23.0	41.0
38	33.910681284325356	35.0	33.0	39.0	24.0	41.0
39	33.927950532874604	35.0	33.0	39.0	24.0	41.0
40	33.74677734262903	35.0	33.0	39.0	23.0	41.0
41	33.78675311204402	35.0	33.0	39.0	23.0	41.0
42	33.715449241515905	35.0	33.0	39.0	23.0	41.0
43	33.584042667534874	35.0	33.0	39.0	23.0	41.0
44	33.548839446808024	35.0	33.0	39.0	23.0	41.0
45	33.47687085265154	35.0	33.0	39.0	23.0	41.0
46	33.44307795215033	35.0	33.0	39.0	23.0	41.0
47	33.43736571173952	35.0	33.0	39.0	23.0	41.0
48	33.457631434227316	35.0	33.0	38.0	23.0	41.0
49	33.45398745477636	35.0	33.0	38.0	24.0	40.0
50	33.209981185295106	35.0	32.0	38.0	24.0	40.0
51	33.02084800786398	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	3.0
13	6.0
14	15.0
15	73.0
16	216.0
17	571.0
18	1241.0
19	2296.0
20	4142.0
21	6520.0
22	9838.0
23	14072.0
24	19275.0
25	26460.0
26	34495.0
27	40910.0
28	45941.0
29	52808.0
30	64130.0
31	77847.0
32	97703.0
33	128904.0
34	219042.0
35	329311.0
36	127425.0
37	147421.0
38	196400.0
39	269302.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.936310710816002	24.32674557781388	29.52489478687625	15.212048924493866
2	29.79730625184573	26.810380541233215	30.173235117025794	13.21907808989526
3	28.605656098917553	26.118264455651875	32.164275435592245	13.111804009838327
4	25.613564953516306	28.346653893955185	31.12205765877451	14.917723493753998
5	22.717634376959865	32.97890102505184	30.024115797569216	14.27934880041908
6	20.827085244283325	40.96069782415191	27.58394353292782	10.628273398636951
7	86.28232701271949	5.527380456707917	6.366215760732887	1.8240767698397047
8	89.03513852235172	3.4988776918959026	5.939571717627072	1.526412068125302
9	85.72738191763897	4.453230901196189	7.269227678799699	2.550159502365143
10	58.06642644786094	20.66147827438998	11.659847249223358	9.612248028525721
11	51.305028837735435	18.23168905543503	17.636516180333157	12.826765926496384
12	44.83070417920197	20.637059855388696	22.867379809724167	11.664856155685161
13	18.96095453060807	47.294877453972845	21.783473321833718	11.960694693585365
14	12.969258880112866	48.004107303298674	27.112428036101694	11.91420578048676
15	11.554868917961416	21.39570047991585	54.273640734096986	12.77578986802575
16	13.527125837296108	16.00643018367034	51.037417574791846	19.429026404241707
17	13.998850038558144	16.76256635496659	28.64045756360529	40.59812604286998
18	20.245373805297547	20.731968197618055	40.04959860919364	18.97305938789076
19	31.961206019453343	22.172028805386244	25.219948387393	20.646816787767417
20	34.36495936002872	21.33361091023309	25.407156266402865	18.894273463335328
21	20.335012360520217	30.75165946114602	28.46733723401924	20.445990944314527
22	22.156323796584136	24.040716148401376	22.75958396857746	31.04337608643703
23	16.997202317036646	32.00033810118617	24.501535542887197	26.500924038889984
24	16.92248612898143	22.086877395535602	45.19098021168891	15.79965626379406
25	15.434580029281234	24.677838615120844	40.79994323239343	19.08763812320449
26	15.086043621314149	37.512900542944585	26.76530038307699	20.63575545266427
27	14.656686420541526	39.980987025888744	27.316280093875257	18.04604645969448
28	13.678280025002792	29.318590451980764	40.74223645586475	16.260893067151695
29	13.339709253850335	24.2726911289136	39.12524666255519	23.262352954680875
30	17.564721854380654	35.660700850366226	29.99865385638839	16.77592343886473
31	32.44832217286363	27.703009413613582	23.4795099202436	16.369158493279194
32	34.139610745356585	23.433647120452722	25.828947931373808	16.597794202816885
33	32.442426272549206	26.66689624154617	21.78743870611598	19.103238779788644
34	19.228409265224727	26.24635680319067	25.732891714746952	28.792342216837646
35	19.770310333060976	24.89066496363847	33.03937313535631	22.299651567944252
36	35.29818124519328	24.972894511386393	23.241639039416963	16.487285204003367
37	19.07261140381908	38.09200317648151	27.007397528730774	15.827987890968629
38	19.611694961770564	37.088447896415815	22.522078320513664	20.777778821299957
39	20.289045208511382	36.110093676986054	25.181598947294827	18.419262167207734
40	27.006145302115325	23.879491971662112	25.074168338910958	24.040194387311605
41	16.129826681401198	23.733764099289047	27.16538678671346	32.97102243259629
42	19.557744865088235	23.288493185278405	25.321274391026545	31.832487558606815
43	19.849670194815154	23.51128517061066	27.614570908897385	29.024473725676803
44	16.537843853602187	28.988263506046692	32.48442804027578	21.989464600075344
45	15.491608516393212	43.56532918428915	21.986073152991832	18.95698914632581
46	21.851771848484752	35.48601523751086	24.47049075804582	18.191722155958566
47	19.448748973435055	29.05541415830023	25.24415810195838	26.25167876630634
48	20.347273746129837	24.79544356475525	34.377846858946064	20.479435830168853
49	21.503496321062556	24.4910481449828	35.48288467097224	18.522570862982406
50	19.902889825971805	38.15362316118348	24.037168172990935	17.906318839853782
51	17.320224607713925	36.50501464583379	24.557468331710655	21.61729241474163
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8592.0
1	7278.5
2	5965.0
3	10510.0
4	15055.0
5	11162.0
6	7269.0
7	7414.5
8	7560.0
9	8208.5
10	8857.0
11	9414.5
12	9972.0
13	9743.0
14	9514.0
15	9437.5
16	9361.0
17	8662.0
18	7963.0
19	7154.0
20	6345.0
21	6555.0
22	6765.0
23	7639.0
24	8513.0
25	8792.5
26	11781.5
27	14491.0
28	15730.5
29	16970.0
30	22396.5
31	27823.0
32	31477.5
33	35132.0
34	44006.5
35	52881.0
36	57411.5
37	61942.0
38	72940.0
39	83938.0
40	120382.5
41	156827.0
42	194612.5
43	232398.0
44	227790.5
45	223183.0
46	200060.5
47	176938.0
48	159403.5
49	141869.0
50	128080.0
51	114291.0
52	103238.5
53	92186.0
54	81778.5
55	71371.0
56	64588.0
57	57805.0
58	55951.5
59	54098.0
60	52876.5
61	51655.0
62	44745.5
63	37836.0
64	33124.5
65	28413.0
66	24368.0
67	20323.0
68	17256.0
69	14189.0
70	12481.5
71	10774.0
72	9527.5
73	8281.0
74	6676.0
75	3877.0
76	2683.0
77	1979.5
78	1276.0
79	923.0
80	570.0
81	380.0
82	190.0
83	217.5
84	245.0
85	158.0
86	71.0
87	56.0
88	41.0
89	26.0
90	11.0
91	9.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1916586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.999320999027184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.70439927082091	13.54920668239313
2	7.3223083506575835	2.4894854021337114
3	2.6076320983683576	1.3298392526259162
4	1.3790797633094454	0.9377367831903877
5	0.8551905688052885	0.7268829497230872
6	0.5840012656456656	0.595657498712929
7	0.44820682478713275	0.5333448181957842
8	0.3420465312167495	0.4651647024605837
9	0.27123904209403243	0.41497915896225934
>10	3.630743360544798	15.108452468124078
>50	1.5757258626222705	18.83999770829757
>100	1.2550781032170268	41.27987165401868
>500	0.0221354162824819	2.3130902321748725
>1k	0.0015811011630344214	0.5473901425572096
>5k	6.324404652137685E-4	0.8689005464298081
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8362	0.43629662326657925	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7802	0.40707800223939866	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4140	0.21600909116522818	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2254	0.11760494963440202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028488155501501106	0.0
2	0.0	0.0	0.0	0.08948202689574065	0.0
3	0.0	0.0	0.0	0.1265270642694875	0.0
4	0.0	0.0	0.0	0.16670266818186086	0.0
5	0.0	0.0	0.0	0.29124704031021825	0.0
6	0.0	0.0	0.0	0.40806934830996366	0.0
7	0.0	0.0	0.0	0.4954121547376429	0.0
8	0.0	0.0	0.0	0.6921682616903181	0.0
9	0.0	0.0	0.0	0.7850417356695708	0.0
10	0.0	0.0	0.0	0.9399004271136281	0.0
11	0.0	0.0	0.0	1.0934025397242806	0.0
12	0.0	0.0	0.0	1.2340171534175872	0.0
13	5.217610897710825E-5	0.0	0.0	1.2918282821642233	0.0
14	5.217610897710825E-5	0.0	0.0	1.313846600152563	0.0
15	5.217610897710825E-5	0.0	0.0	1.3435869822695146	0.0
16	5.217610897710825E-5	0.0	0.0	1.411781156702595	0.0
17	5.217610897710825E-5	0.0	0.0	1.4938020000146093	0.0
18	5.217610897710825E-5	0.0	0.0	1.6380167652273365	0.0
19	5.217610897710825E-5	0.0	0.0	1.691288572492964	0.0
20	1.043522179542165E-4	0.0	0.0	1.753847727156517	0.0
21	1.5652832693132478E-4	0.0	0.0	1.8313814250964997	0.0
22	1.5652832693132478E-4	0.0	0.0	1.9126718028828344	0.0
23	1.5652832693132478E-4	0.0	0.0	2.021041581228288	0.0
24	2.6088054488554126E-4	0.0	0.0	2.0992535685849734	0.0
25	2.6088054488554126E-4	0.0	0.0	2.1554994140622963	0.0
26	2.6088054488554126E-4	0.0	0.0	2.22291094686072	0.0
27	2.6088054488554126E-4	0.0	0.0	2.285104868761433	0.0
28	2.6088054488554126E-4	0.0	0.0	2.3534555715214447	0.0
29	2.6088054488554126E-4	0.0	0.0	2.4304675083716565	0.0
30	2.6088054488554126E-4	0.0	0.0	2.545724533102089	0.0
31	2.6088054488554126E-4	0.0	0.0	2.6357804971965777	0.0
32	2.6088054488554126E-4	0.0	0.0	2.7209840831561953	0.0
33	2.6088054488554126E-4	0.0	0.0	2.8062920213337677	0.0
34	2.6088054488554126E-4	0.0	0.0	2.8983828536783633	0.0
35	3.1305665386264955E-4	0.0	0.0	3.0278839561595463	0.0
36	3.1305665386264955E-4	0.0	0.0	3.1225314178440207	0.0
37	3.1305665386264955E-4	0.0	0.0	3.2158223006950903	0.0
38	3.1305665386264955E-4	0.0	0.0	3.3090610074371827	0.0
39	3.1305665386264955E-4	0.0	0.0	3.406630331224375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTACG	30	2.1662636E-6	45.000004	1
CTACGAT	60	0.0	45.000004	36
CTATCGA	45	3.8562575E-10	45.0	25
CGACTTA	20	7.0344686E-4	45.0	17
ATCGCTA	20	7.0344686E-4	45.0	41
TGCCGTA	20	7.0344686E-4	45.0	31
CGTATGG	195	0.0	42.69231	2
TGTATAC	70	0.0	41.785713	13
CGTTTTT	6195	0.0	40.569008	1
GTAGGGC	585	0.0	40.384613	4
TTCGTAC	175	0.0	39.857143	33
GTCGTTT	85	0.0	39.705883	9
AGGGCGA	1250	0.0	39.42	6
TCCGATT	40	3.459827E-7	39.375	40
GCGATAT	160	0.0	39.375	9
TAATGCG	80	0.0	39.375	1
TATGGGA	1405	0.0	38.91459	4
TAGCGGG	1285	0.0	38.871597	3
CGTTGAT	330	0.0	38.863636	25
GGACTAA	3060	0.0	38.82353	8
>>END_MODULE