FastQCFastQC Report
Thu 26 May 2016
SRR1545201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545201_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2001202
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112200.5606630415120513No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107190.5356280875194008No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28830.14406341788585061No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27780.13881657124068433No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24220.12102726261516829No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22960.11473104664096877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAT302.1663109E-645.00000415
CTAAGCG453.8562575E-1045.01
TACGCTA207.034541E-445.037
CCCGTAT406.8193913E-945.045
TATTCCG253.8918457E-545.01
ACTACGT253.8918457E-545.041
CGTAATC253.8918457E-545.029
TTACGTA253.8918457E-545.015
CGCGTAA253.8918457E-545.031
TAATGCG253.8918457E-545.01
TAGTCGA253.8918457E-545.015
AATCGTC207.034541E-445.030
AATCGGC207.034541E-445.019
AATACGG1000.042.752
GTATGCG1300.041.5384641
TCGTATA603.6379788E-1241.25000429
TATCGTG603.6379788E-1241.2500041
AAGCCGT556.184564E-1140.90909238
TACGGGA3700.040.7432444
CTATACG501.0822987E-940.51