FastQCFastQC Report
Thu 26 May 2016
SRR1545200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545200_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1282848
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59290.46217478610092544No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29450.22956733767367604No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT207.0335926E-445.00000419
CGTGCCT207.0335926E-445.00000440
TTTCGCG207.0335926E-445.00000442
ACCGAAA207.0335926E-445.00000421
TTCGTAA207.0335926E-445.0000041
CTAGTTC207.0335926E-445.00000417
TACGATG207.0335926E-445.0000041
ACCAACG207.0335926E-445.0000041
TCGCGAC207.0335926E-445.00000425
GTACGTA406.8157533E-945.00000411
GTACGAT207.0335926E-445.00000414
GCTTTAC207.0335926E-445.00000437
TATCCGT207.0335926E-445.00000432
AGCACGT207.0335926E-445.00000436
CCTTACG253.89106E-545.040
GTACCGG253.89106E-545.022
GATGTAC351.2120836E-745.09
CACGGTC253.89106E-545.040
GTCGAAG253.89106E-545.01
GTTTCGA453.8562575E-1045.017