FastQCFastQC Report
Thu 26 May 2016
SRR1545199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545199_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1122902
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49170.43788327031210206No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27700.24668225722280307No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGC207.033216E-445.00000440
CGTTGAT600.045.00000425
TAACGTG302.165456E-645.00000438
TAACGCG302.165456E-645.0000041
ACGGGTA750.045.0000045
CTAACGT302.165456E-645.00000437
CCCGTCG302.165456E-645.00000419
GTAGCAC207.033216E-445.0000049
CTAGTCG406.8139343E-945.00000430
GTATGCG406.8139343E-945.0000041
TATTACG207.033216E-445.0000041
CGTACTC207.033216E-445.00000437
TCCCGTA207.033216E-445.00000424
GAGCCGT207.033216E-445.00000418
CCGAATT207.033216E-445.00000424
CCGAATA207.033216E-445.00000436
CGCACTC207.033216E-445.00000431
TCATACC207.033216E-445.00000438
CGGTCAT302.165456E-645.00000428
CGCAAGT302.165456E-645.00000416