FastQCFastQC Report
Thu 26 May 2016
SRR1545198_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545198_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1296540
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44670.34453237077143783No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18220.14052786647538834No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG16850.12996128156478012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC207.0336205E-445.00000439
GGTACGA207.0336205E-445.00000435
TACGGAT207.0336205E-445.00000433
CGTAGTA207.0336205E-445.00000417
ACTACGT207.0336205E-445.00000434
CGTACAA351.2120836E-745.00000445
CGAGAGT207.0336205E-445.00000421
CACCGTA207.0336205E-445.00000414
TCGACGA207.0336205E-445.00000435
GCCCGTT207.0336205E-445.00000434
GTACGTA207.0336205E-445.00000412
GTCGTAA253.891084E-545.031
TATAGCG253.891084E-545.01
CGACTAT253.891084E-545.032
GCTCGAT253.891084E-545.011
ACGTTAT302.165718E-644.99999644
CAATTCG302.165718E-644.99999627
CGTCAAG302.165718E-644.99999628
TACGTAC302.165718E-644.99999637
TTAACGG950.042.631582