##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545198_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1296540
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03319064587286	31.0	31.0	33.0	30.0	34.0
2	31.299106853625805	31.0	31.0	34.0	28.0	34.0
3	31.40932713221343	31.0	31.0	34.0	30.0	34.0
4	35.191093988615854	37.0	35.0	37.0	32.0	37.0
5	35.034194857081154	35.0	35.0	37.0	32.0	37.0
6	35.097420056457956	37.0	35.0	37.0	32.0	37.0
7	35.742759961127305	37.0	35.0	37.0	35.0	37.0
8	35.75158344516945	37.0	35.0	37.0	35.0	37.0
9	37.47467336140806	39.0	37.0	39.0	35.0	39.0
10	36.57462091412529	39.0	35.0	39.0	32.0	39.0
11	36.27393755688216	38.0	35.0	39.0	31.0	39.0
12	35.470725932096194	37.0	35.0	39.0	30.0	39.0
13	35.096622549246455	37.0	34.0	39.0	29.0	39.0
14	35.95769818131334	38.0	34.0	40.0	28.0	41.0
15	36.296144353432986	38.0	35.0	40.0	30.0	41.0
16	36.4254284480232	38.0	35.0	40.0	31.0	41.0
17	36.369302142625756	38.0	35.0	40.0	31.0	41.0
18	36.309849291190396	38.0	35.0	40.0	30.0	41.0
19	36.231815447267344	38.0	35.0	40.0	30.0	41.0
20	36.11424406497293	38.0	34.0	40.0	30.0	41.0
21	35.97311382602928	37.0	34.0	40.0	30.0	41.0
22	35.900762799450845	37.0	34.0	40.0	30.0	41.0
23	35.87134141638515	37.0	34.0	40.0	30.0	41.0
24	35.821554290650504	37.0	34.0	40.0	30.0	41.0
25	35.706667746463665	37.0	34.0	40.0	29.0	41.0
26	35.552655529331915	37.0	34.0	40.0	29.0	41.0
27	35.46613756613757	37.0	34.0	40.0	29.0	41.0
28	35.481524673361406	37.0	34.0	40.0	29.0	41.0
29	35.46905456059975	37.0	34.0	40.0	29.0	41.0
30	35.38719052246749	37.0	34.0	40.0	29.0	41.0
31	35.211601647461706	37.0	34.0	40.0	28.0	41.0
32	35.088864207814645	37.0	34.0	40.0	27.0	41.0
33	34.92304672435868	37.0	33.0	40.0	27.0	41.0
34	34.77031869437117	36.0	33.0	40.0	26.0	41.0
35	34.60496938004226	36.0	33.0	40.0	25.0	41.0
36	34.447998519135545	36.0	33.0	40.0	24.0	41.0
37	34.387695713205915	36.0	33.0	40.0	24.0	41.0
38	34.348395730174154	36.0	33.0	40.0	24.0	41.0
39	34.301419933052586	36.0	33.0	40.0	24.0	41.0
40	34.15813704166474	36.0	33.0	40.0	23.0	41.0
41	34.09142024156601	36.0	33.0	40.0	23.0	41.0
42	34.05535193669304	35.0	33.0	40.0	23.0	41.0
43	33.98941567556728	35.0	33.0	40.0	23.0	41.0
44	33.88080892220834	35.0	33.0	40.0	23.0	41.0
45	33.79178351612754	35.0	33.0	39.0	23.0	41.0
46	33.7511175898931	35.0	32.0	39.0	23.0	41.0
47	33.62793280577537	35.0	32.0	39.0	23.0	41.0
48	33.575940580313755	35.0	32.0	39.0	23.0	41.0
49	33.5434803399818	35.0	32.0	39.0	23.0	41.0
50	33.469804248229906	35.0	32.0	39.0	23.0	41.0
51	33.30248584694649	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	19.0
15	53.0
16	145.0
17	403.0
18	924.0
19	1740.0
20	2903.0
21	4593.0
22	6850.0
23	9817.0
24	13591.0
25	18041.0
26	23087.0
27	27392.0
28	31403.0
29	36582.0
30	43019.0
31	51653.0
32	63860.0
33	80367.0
34	122643.0
35	161862.0
36	100171.0
37	121483.0
38	160391.0
39	213434.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.78311506008299	24.249463957918767	28.176917025313525	12.790503956684715
2	30.63962546469835	25.45791105557869	31.07740601909698	12.825057460625974
3	29.261881623397656	25.85998118068089	32.063337806778044	12.814799389143413
4	25.584632946148982	27.906736390701404	31.24855384330603	15.260076819843585
5	22.810788714578802	32.58927607324109	30.648340969040678	13.951594243139432
6	20.151171579743007	42.72085705030311	27.24736606660805	9.880605303345828
7	89.47753250960247	3.39526740401376	5.670014037360977	1.4571860490227837
8	91.05041109414287	2.5811004673978433	5.099495580545143	1.2689928579141407
9	87.29071220324865	4.207968901846453	6.343344594073456	2.1579743008314436
10	46.209835408086136	29.339164237123423	12.501735388032763	11.949264966757678
11	42.89786662964505	21.45518071174048	19.79468431363475	15.852268344979715
12	37.90951301155383	21.442068891048482	24.489641661653323	16.158776435744365
13	24.035509895568204	32.95015965569901	25.911965693306797	17.10236475542598
14	18.447329044996682	35.36713097937588	29.94061116510096	16.244928810526478
15	17.854751878075493	22.13876933993552	42.182346861647154	17.82413192034183
16	20.317691702531352	18.95853579527049	39.38883489903898	21.334937603159176
17	21.026655560183258	18.957995896771408	27.483301710706957	32.53204683233838
18	24.727428386320515	21.48772887839943	32.73173214864177	21.053110586638283
19	29.030573680719453	24.28046955743749	24.794221543492682	21.894735218350377
20	30.857821586684558	24.374026254492726	24.24684159378037	20.521310565042345
21	25.044657318709795	26.006293673932156	28.495997038271092	20.453051969086957
22	24.12443889120274	21.95913739645518	27.701806346121217	26.214617366220867
23	21.292285621731686	27.336372190599594	26.3896216082805	24.981720579388217
24	21.647924475912813	25.028151850309282	33.54289107933423	19.781032594443673
25	21.619695497246518	24.261881623397656	32.274360991562155	21.844061887793668
26	20.253135267712526	31.144507689697193	25.24935597821895	23.353001064371327
27	20.018356548968793	29.958273558856654	28.4723957610255	21.550974131149058
28	17.65151865734956	27.637789809801472	34.0499328983294	20.660758634519567
29	19.45447113085597	24.79900350162741	33.401283415860675	22.345241951655943
30	22.346167491940086	27.43447946071853	30.08823484042143	20.131118206919957
31	29.67073904391689	24.397550403381306	24.44698968022583	21.484720872475975
32	29.95426288429204	25.05854042297191	25.617181112807934	19.370015579928115
33	28.101485492156048	25.517377018834747	25.295555864068987	21.085581624940225
34	21.309253860274268	26.939778178845238	27.643420179863327	24.107547781017168
35	22.043824332454072	25.374072531506936	29.93883721288969	22.643265923149304
36	30.71899054406343	25.09193700155799	24.203264072068738	19.985808382309838
37	22.134064510157806	30.935721227266416	26.032054545174077	20.898159717401697
38	22.953244789979486	31.20713591559073	22.901414534067595	22.938204760362193
39	22.319866722199084	28.36341339256791	25.59273142363521	23.72398846159779
40	24.861014700664846	25.214108319064586	25.12625912042822	24.798617859842352
41	20.299412281919572	23.786693815848334	26.992225461613216	28.92166844061888
42	22.963271476391007	25.63869992441421	24.49249541086276	26.905533188332022
43	22.707128202755026	25.76727289555278	26.051336634427013	25.474262267265185
44	22.548475172381877	27.358430900704956	27.29341169574406	22.79968223116911
45	20.92299504835948	31.854551344347264	23.75993027596526	23.462523331327997
46	24.24475912814105	28.485121939932434	25.989865333888655	21.280253598037856
47	22.52518240856433	27.388819473367576	26.510790257145946	23.575207860922145
48	22.810094559365695	26.469449457787654	28.298856957749084	22.421599025097567
49	22.62136146975797	24.62114551035834	29.76892344239283	22.98856957749086
50	22.01189319265121	29.434957656532003	26.279636571181758	22.273512579635028
51	20.968809292424453	30.54568312585805	25.337128048498307	23.14837953321918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	568.0
1	1014.0
2	1460.0
3	4715.0
4	7970.0
5	5903.0
6	3836.0
7	4150.5
8	4465.0
9	4736.0
10	5007.0
11	5119.5
12	5232.0
13	5130.5
14	5029.0
15	4739.5
16	4450.0
17	4226.0
18	4002.0
19	3829.0
20	3656.0
21	3888.5
22	4121.0
23	4069.0
24	4017.0
25	4342.5
26	6757.5
27	8847.0
28	9661.0
29	10475.0
30	12875.5
31	15276.0
32	18296.5
33	21317.0
34	22513.5
35	23710.0
36	29599.5
37	35489.0
38	42315.5
39	49142.0
40	62097.0
41	75052.0
42	89901.5
43	104751.0
44	107594.5
45	110438.0
46	107471.0
47	104504.0
48	99471.5
49	94439.0
50	94722.5
51	95006.0
52	89584.0
53	84162.0
54	80000.5
55	75839.0
56	77247.5
57	78656.0
58	74844.5
59	71033.0
60	63486.5
61	55940.0
62	48886.5
63	41833.0
64	35752.5
65	29672.0
66	24119.0
67	18566.0
68	15590.0
69	12614.0
70	11109.0
71	9604.0
72	7400.0
73	5196.0
74	4233.5
75	2518.0
76	1765.0
77	1255.5
78	746.0
79	596.5
80	447.0
81	292.0
82	137.0
83	116.5
84	96.0
85	59.0
86	22.0
87	14.0
88	6.0
89	4.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1296540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.227380113146392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35725915797072	15.066048064546242
2	7.3535898682169725	2.827805351847797
3	2.6355968254442628	1.5202686596345634
4	1.4937069136651486	1.148802824266982
5	0.9822682777117959	0.9443222774325173
6	0.7461980726745427	0.8608460389806403
7	0.5488909528405442	0.738761449365258
8	0.4756246997990284	0.7316013515389649
9	0.373565159857583	0.6464411393049039
>10	4.428647370397295	19.670928850624836
>50	1.4269701205516723	19.907946456827048
>100	1.1724555840963558	34.72588079632684
>500	0.003216613399441305	0.4138340633298105
>1k	0.0020103833746508153	0.7965126759736665
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4467	0.34453237077143783	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1822	0.14052786647538834	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1685	0.12996128156478012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024295432458697766	0.0
2	0.0	0.0	0.0	0.09263115677109846	0.0
3	0.0	0.0	0.0	0.12764743085442795	0.0
4	0.0	0.0	0.0	0.1891958597497956	0.0
5	7.712835701173894E-5	0.0	0.0	0.3359711231431348	0.0
6	7.712835701173894E-5	0.0	0.0	0.47271970012494796	0.0
7	7.712835701173894E-5	0.0	0.0	0.5591034599780955	0.0
8	7.712835701173894E-5	0.0	0.0	0.7796905610316689	0.0
9	7.712835701173894E-5	0.0	0.0	0.8578215866845604	0.0
10	7.712835701173894E-5	0.0	0.0	1.0207938050503649	0.0
11	7.712835701173894E-5	0.0	0.0	1.1766702145710892	0.0
12	7.712835701173894E-5	0.0	0.0	1.3166581825473953	0.0
13	7.712835701173894E-5	0.0	0.0	1.3748129637342466	0.0
14	7.712835701173894E-5	0.0	0.0	1.396794545482592	0.0
15	7.712835701173894E-5	0.0	0.0	1.4312709210668395	0.0
16	7.712835701173894E-5	0.0	0.0	1.5127184660712358	0.0
17	7.712835701173894E-5	0.0	0.0	1.6061980347694633	0.0
18	7.712835701173894E-5	0.0	0.0	1.7533589399478613	0.0
19	7.712835701173894E-5	0.0	0.0	1.8156015240563346	0.0
20	7.712835701173894E-5	0.0	0.0	1.8895676184305923	0.0
21	7.712835701173894E-5	0.0	0.0	1.983278572199855	0.0
22	7.712835701173894E-5	0.0	0.0	2.0775294244682	0.0
23	7.712835701173894E-5	0.0	0.0	2.1851234824995758	0.0
24	7.712835701173894E-5	0.0	0.0	2.2670337976460426	0.0
25	7.712835701173894E-5	0.0	0.0	2.337220602526725	0.0
26	7.712835701173894E-5	0.0	0.0	2.4111866969009825	0.0
27	7.712835701173894E-5	0.0	0.0	2.49224860012032	0.0
28	7.712835701173894E-5	0.0	0.0	2.5787866166874913	0.0
29	7.712835701173894E-5	0.0	0.0	2.672189057028707	0.0
30	7.712835701173894E-5	0.0	0.0	2.799296589384053	0.0
31	7.712835701173894E-5	0.0	0.0	2.905656593703241	0.0
32	1.5425671402347787E-4	0.0	0.0	2.9968223116911163	0.0
33	1.5425671402347787E-4	0.0	0.0	3.0951609668810836	0.0
34	1.5425671402347787E-4	0.0	0.0	3.204606105480741	0.0
35	1.5425671402347787E-4	0.0	0.0	3.3349530288305798	0.0
36	1.5425671402347787E-4	0.0	0.0	3.4356055347308994	0.0
37	1.5425671402347787E-4	0.0	0.0	3.5386490196985823	0.0
38	1.5425671402347787E-4	0.0	0.0	3.6696129699045152	0.0
39	1.5425671402347787E-4	0.0	0.0	3.871457880204236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	20	7.0336205E-4	45.000004	39
GGTACGA	20	7.0336205E-4	45.000004	35
TACGGAT	20	7.0336205E-4	45.000004	33
CGTAGTA	20	7.0336205E-4	45.000004	17
ACTACGT	20	7.0336205E-4	45.000004	34
CGTACAA	35	1.2120836E-7	45.000004	45
CGAGAGT	20	7.0336205E-4	45.000004	21
CACCGTA	20	7.0336205E-4	45.000004	14
TCGACGA	20	7.0336205E-4	45.000004	35
GCCCGTT	20	7.0336205E-4	45.000004	34
GTACGTA	20	7.0336205E-4	45.000004	12
GTCGTAA	25	3.891084E-5	45.0	31
TATAGCG	25	3.891084E-5	45.0	1
CGACTAT	25	3.891084E-5	45.0	32
GCTCGAT	25	3.891084E-5	45.0	11
ACGTTAT	30	2.165718E-6	44.999996	44
CAATTCG	30	2.165718E-6	44.999996	27
CGTCAAG	30	2.165718E-6	44.999996	28
TACGTAC	30	2.165718E-6	44.999996	37
TTAACGG	95	0.0	42.63158	2
>>END_MODULE