FastQCFastQC Report
Thu 26 May 2016
SRR1545197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545197_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences944734
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41490.43917123761820787No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27010.28590058153935394No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGA207.0326467E-445.00000442
CCCTGCG351.2116288E-745.00000416
TGATCCC207.0326467E-445.00000424
AAATCGT207.0326467E-445.00000412
GTCGAAG207.0326467E-445.0000041
CTCCGAT207.0326467E-445.00000441
GCGCGAA351.2116288E-745.00000430
CGCATCC207.0326467E-445.00000427
GAGTACG207.0326467E-445.0000041
CGCGAAA351.2116288E-745.00000431
GACCCGT406.8121153E-945.00000427
TCAGGCG406.8121153E-945.00000420
ATCCGGC207.0326467E-445.00000421
ACCTACG207.0326467E-445.00000416
CCATCGG207.0326467E-445.00000415
CGTAGTG207.0326467E-445.00000421
CACTAGT207.0326467E-445.00000441
CGAGGTA207.0326467E-445.0000042
AGGCACG207.0326467E-445.0000041
CGCTAAT207.0326467E-445.00000424