##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545197_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	944734
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08454125711576	31.0	31.0	33.0	30.0	34.0
2	31.332418437359088	31.0	31.0	34.0	28.0	34.0
3	31.426507355509592	31.0	31.0	34.0	30.0	34.0
4	35.20850842671059	37.0	35.0	37.0	32.0	37.0
5	35.057238333753205	37.0	35.0	37.0	32.0	37.0
6	35.1061558068197	37.0	35.0	37.0	32.0	37.0
7	35.767720861110114	37.0	35.0	37.0	35.0	37.0
8	35.78790220315983	37.0	35.0	37.0	35.0	37.0
9	37.5178928671986	39.0	37.0	39.0	35.0	39.0
10	36.672452775066844	39.0	35.0	39.0	32.0	39.0
11	36.255044277013425	38.0	35.0	39.0	31.0	39.0
12	34.979984842294236	35.0	34.0	39.0	30.0	39.0
13	34.39900331733588	35.0	33.0	39.0	25.0	39.0
14	35.24507850887128	36.0	33.0	40.0	26.0	41.0
15	35.74087203382116	36.0	33.0	40.0	30.0	41.0
16	36.00813456486164	36.0	34.0	40.0	31.0	41.0
17	35.980409300395664	36.0	34.0	40.0	31.0	41.0
18	35.92870162394917	36.0	34.0	40.0	30.0	41.0
19	35.8195333289582	36.0	34.0	40.0	30.0	41.0
20	35.654331272082935	36.0	34.0	40.0	30.0	41.0
21	35.48082634900406	35.0	34.0	40.0	30.0	41.0
22	35.370895934728715	35.0	34.0	40.0	30.0	41.0
23	35.370899110225736	35.0	34.0	40.0	30.0	41.0
24	35.29526829774307	35.0	34.0	40.0	29.0	41.0
25	35.23408070419822	35.0	34.0	40.0	29.0	41.0
26	35.077538227691605	35.0	34.0	40.0	29.0	41.0
27	34.999083339860746	35.0	34.0	40.0	29.0	41.0
28	35.03238795258771	35.0	34.0	40.0	29.0	41.0
29	35.081925706071765	36.0	34.0	40.0	29.0	41.0
30	35.02772632296498	36.0	34.0	40.0	29.0	41.0
31	34.79281681404501	35.0	34.0	40.0	29.0	41.0
32	34.56141940482718	35.0	33.0	40.0	27.0	41.0
33	34.3544817906416	35.0	33.0	40.0	26.0	41.0
34	34.238858768711616	35.0	33.0	40.0	25.0	41.0
35	34.077411207810876	35.0	33.0	40.0	24.0	41.0
36	33.84024074501394	35.0	33.0	39.0	23.0	41.0
37	33.75230064758969	35.0	33.0	39.0	23.0	41.0
38	33.7869379105653	35.0	33.0	39.0	23.0	41.0
39	33.76447021066247	35.0	33.0	39.0	23.0	41.0
40	33.57778697495803	35.0	33.0	39.0	23.0	41.0
41	33.586004102742145	35.0	33.0	39.0	23.0	41.0
42	33.563162752690175	35.0	33.0	39.0	23.0	41.0
43	33.46808096247197	35.0	33.0	39.0	23.0	41.0
44	33.37557979283057	35.0	32.0	39.0	23.0	41.0
45	33.31966669983297	35.0	32.0	39.0	23.0	41.0
46	33.26341806265044	35.0	32.0	39.0	23.0	41.0
47	33.167651423575315	35.0	32.0	38.0	23.0	40.0
48	33.17201349797933	35.0	32.0	38.0	23.0	40.0
49	33.200338931381744	35.0	32.0	38.0	23.0	40.0
50	33.08098787595238	35.0	32.0	38.0	23.0	40.0
51	32.916136182248124	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	13.0
15	34.0
16	114.0
17	328.0
18	721.0
19	1471.0
20	2334.0
21	3772.0
22	5436.0
23	7859.0
24	11008.0
25	14778.0
26	18434.0
27	21398.0
28	23637.0
29	27468.0
30	32465.0
31	39728.0
32	49460.0
33	63113.0
34	107269.0
35	157141.0
36	60932.0
37	69460.0
38	92611.0
39	133627.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.412298064852116	24.708542298678783	29.688356722633035	13.190802913836064
2	30.258040887699604	26.694392283965644	29.889259833985015	13.158306994349733
3	27.43481233871121	25.523586533352244	33.22501360171223	13.816587526224314
4	25.32331852140391	28.486854500843624	31.862513681099653	14.327313296652813
5	22.510039863072567	32.95943620108941	30.981736658149277	13.548787277688746
6	19.429172656006877	42.06411540179564	27.7882451568378	10.718466785359688
7	88.020755048511	4.412141406999219	6.282932550326335	1.2841709941634365
8	89.72948999400889	2.9594573710695284	5.742039558224855	1.569013076696721
9	86.45354142012461	4.47512209786035	7.030126998710748	2.041209483304295
10	55.919549841542704	23.98272953021697	12.007083475348617	8.090637152891714
11	52.538492316355715	18.547442983951036	18.690234499869803	10.223830199823443
12	46.022689984694104	22.11384368510078	20.38415045928272	11.479315870922397
13	20.679683381777306	47.70930230096514	19.958633858842806	11.652380458414749
14	13.701740384065781	48.35519839446871	25.2027554846126	12.740305736852914
15	11.501438500149249	23.14027017128631	51.913766202973534	13.444525125590905
16	12.743587083771729	17.902287839751718	50.5230043588989	18.831120717577647
17	14.018654986482968	18.828580319963077	27.18945226910432	39.96331242444963
18	20.280100006986093	23.23130108580828	36.01680473022036	20.471794176985266
19	28.770638084370837	24.84572376986538	24.6036450471773	21.77999309858648
20	31.641393238731748	23.669307974519814	23.895297512315636	20.7940012744328
21	20.106612019891312	31.353799058782684	26.982939113020173	21.55664980830583
22	21.73500689082853	26.52323299468422	24.211153615726754	27.530606498760495
23	16.711370608023	32.38731748830888	24.616558735051346	26.284753168616774
24	17.69376353555604	24.604915245984586	41.70284969102414	15.998471527435235
25	13.418168500339778	27.085295966907086	38.33195375629542	21.164581776457712
26	14.575848863277919	38.13412029206105	27.034805564317576	20.25522528034346
27	14.838356616783138	38.55614384578093	28.15946075826635	18.446038779169584
28	12.548399867052526	30.867630465294994	39.841055789248614	16.74291387840387
29	13.331477431742691	24.544474952738017	39.33615176335349	22.787895852165796
30	17.10333278997051	34.028837746921354	30.769295907631143	18.09853355547699
31	30.595384520933933	28.06451339742192	23.147997214030617	18.19210486761353
32	30.965647473257025	27.08201461998827	25.230488158571617	16.721849748183086
33	28.47595196108111	29.034521886583946	23.686879058020565	18.802647094314377
34	17.841741696604547	28.688710261301065	27.165847741268973	26.303700300825415
35	18.578351154928267	27.21496209515059	30.291912855893827	23.914773894027313
36	30.64566322372223	25.3460762500344	25.220961667516995	18.787298858726373
37	18.399994072405566	35.29289725996947	28.774236980991475	17.532871686633484
38	19.238748684814986	34.67420459092189	23.652054440720878	22.434992283542247
39	18.83069731797522	34.412967036223954	26.26548848670631	20.490847159094518
40	24.34431279069029	26.750175181585504	23.9588074526798	24.946704575044404
41	15.467210876288986	25.56391534548349	26.779389754153023	32.1894840240745
42	20.747956567668783	26.81643721936545	23.021400732904713	29.414205480061057
43	21.213166880836297	26.726358953948942	25.52104613573768	26.53942802947708
44	18.834296214595856	31.906758939553352	28.14792311910019	21.111021726750597
45	14.874663132691317	42.0940709236674	21.366014137312725	21.665251806328552
46	21.654560966367253	34.34956294576039	25.42789822320357	18.567977864668787
47	19.916717298202457	29.618284088431242	25.99948768648106	24.46551092688524
48	21.295412253607893	25.78217784053501	30.92394261241789	21.998467293439212
49	20.670368590523893	23.98971562365703	32.861419193127375	22.478496592691698
50	18.46509176127884	36.204900003598894	24.76855919232292	20.56144904279935
51	16.895020185576044	36.221835987696004	23.670260623625275	23.21288320310267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	816.0
1	953.5
2	1091.0
3	3630.0
4	6169.0
5	4603.0
6	3037.0
7	3486.5
8	3936.0
9	4395.5
10	4855.0
11	5389.0
12	5923.0
13	6053.0
14	6183.0
15	5902.5
16	5622.0
17	5293.0
18	4964.0
19	4831.5
20	4699.0
21	4490.5
22	4282.0
23	4383.0
24	4484.0
25	5054.0
26	6251.0
27	6878.0
28	7943.5
29	9009.0
30	11182.5
31	13356.0
32	16297.0
33	19238.0
34	22427.5
35	25617.0
36	26761.0
37	27905.0
38	34084.5
39	40264.0
40	57750.0
41	75236.0
42	91195.5
43	107155.0
44	106685.5
45	106216.0
46	98658.5
47	91101.0
48	85069.5
49	79038.0
50	74229.5
51	69421.0
52	63646.0
53	57871.0
54	45787.0
55	33703.0
56	32697.5
57	31692.0
58	29431.5
59	27171.0
60	23890.5
61	20610.0
62	17182.0
63	13754.0
64	12496.5
65	11239.0
66	8390.0
67	5541.0
68	4547.5
69	3554.0
70	3265.0
71	2976.0
72	2331.5
73	1687.0
74	1526.0
75	1029.0
76	693.0
77	629.5
78	566.0
79	338.5
80	111.0
81	79.5
82	48.0
83	30.0
84	12.0
85	13.0
86	14.0
87	8.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	944734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.575504923315446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76618077674902	15.810387694326359
2	8.002395965881549	3.1330188325686783
3	2.7213526613217134	1.5981555725934233
4	1.36085170054789	1.0655743665590967
5	0.8085958561198009	0.7914336081222816
6	0.5024063476605393	0.590091475928029
7	0.39873829554611046	0.5463852427304106
8	0.3014655004789086	0.4721071511067703
9	0.2613619897542244	0.4604663625481207
>10	2.772719737606863	11.956476934730633
>50	0.751055129474689	10.650444563472291
>100	1.3431079808163688	51.14652439791214
>500	0.00868271825982299	1.0512557541554963
>1k	0.0010853397824778738	0.7276780432462763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4149	0.43917123761820787	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2701	0.28590058153935394	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0405405119324593	0.0
2	0.0	0.0	0.0	0.16745454276018434	0.0
3	0.0	0.0	0.0	0.24398402089900437	0.0
4	0.0	0.0	0.0	0.3612657107714976	0.0
5	0.0	0.0	0.0	0.6044029324656465	0.0
6	0.0	0.0	0.0	0.8370610139997079	0.0
7	0.0	0.0	0.0	0.9729722863790231	0.0
8	0.0	0.0	0.0	1.3034356760739	0.0
9	0.0	0.0	0.0	1.43468955282651	0.0
10	0.0	0.0	0.0	1.6688295329690686	0.0
11	0.0	0.0	0.0	1.9121784544644314	0.0
12	0.0	0.0	0.0	2.1173155618406874	0.0
13	0.0	0.0	0.0	2.1977614863019643	0.0
14	0.0	0.0	0.0	2.2290930568816196	0.0
15	0.0	0.0	0.0	2.285087654302693	0.0
16	0.0	0.0	0.0	2.417082480359551	0.0
17	0.0	0.0	0.0	2.5724701344505436	0.0
18	0.0	0.0	0.0	2.8122201593252703	0.0
19	0.0	0.0	0.0	2.9109781165915485	0.0
20	0.0	0.0	0.0	3.0067722766408322	0.0
21	0.0	0.0	0.0	3.126594364127892	0.0
22	0.0	0.0	0.0	3.2545668939616865	0.0
23	0.0	0.0	0.0	3.411013047058749	0.0
24	0.0	0.0	0.0	3.5198267448826868	0.0
25	0.0	0.0	0.0	3.6076821623864497	0.0
26	0.0	0.0	0.0	3.7086629675654734	0.0
27	0.0	0.0	0.0	3.8020225799007976	0.0
28	0.0	0.0	0.0	3.894323693230052	0.0
29	0.0	0.0	0.0	3.9978448960236426	0.0
30	0.0	0.0	0.0	4.140001312538767	0.0
31	0.0	0.0	0.0	4.26839724197499	0.0
32	0.0	0.0	0.0	4.3776343394013555	0.0
33	0.0	0.0	0.0	4.487824085933183	0.0
34	0.0	0.0	0.0	4.606058424911139	0.0
35	0.0	0.0	0.0	4.768326322541583	0.0
36	0.0	0.0	0.0	4.895028653568095	0.0
37	0.0	0.0	0.0	5.025224031314635	0.0
38	0.0	0.0	0.0	5.155313559160568	0.0
39	0.0	0.0	0.0	5.288155184422282	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	20	7.0326467E-4	45.000004	42
CCCTGCG	35	1.2116288E-7	45.000004	16
TGATCCC	20	7.0326467E-4	45.000004	24
AAATCGT	20	7.0326467E-4	45.000004	12
GTCGAAG	20	7.0326467E-4	45.000004	1
CTCCGAT	20	7.0326467E-4	45.000004	41
GCGCGAA	35	1.2116288E-7	45.000004	30
CGCATCC	20	7.0326467E-4	45.000004	27
GAGTACG	20	7.0326467E-4	45.000004	1
CGCGAAA	35	1.2116288E-7	45.000004	31
GACCCGT	40	6.8121153E-9	45.000004	27
TCAGGCG	40	6.8121153E-9	45.000004	20
ATCCGGC	20	7.0326467E-4	45.000004	21
ACCTACG	20	7.0326467E-4	45.000004	16
CCATCGG	20	7.0326467E-4	45.000004	15
CGTAGTG	20	7.0326467E-4	45.000004	21
CACTAGT	20	7.0326467E-4	45.000004	41
CGAGGTA	20	7.0326467E-4	45.000004	2
AGGCACG	20	7.0326467E-4	45.000004	1
CGCTAAT	20	7.0326467E-4	45.000004	24
>>END_MODULE