FastQCFastQC Report
Thu 26 May 2016
SRR1545196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545196_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1516849
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77830.5131031500169101No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19380.12776485991684078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.0339994E-445.0000041
CGTTACA207.0339994E-445.00000439
GACGATA207.0339994E-445.0000049
TAGTACG750.045.0000041
TCTATCG207.0339994E-445.00000432
AACGTTG453.8562575E-1045.01
CGACACG253.8913975E-545.026
CGCATTA302.1659635E-644.99999616
GGCGTAG1300.043.269231
CGTTTTT53100.043.0084721
CTAGACG800.042.1875041
ATACTCG800.042.18750444
CGGTCTA5750.041.86956431
TAGGGTA10100.041.6584135
ACGGGAT6600.041.5909085
GCGTAAG650.041.538461
CGACGGT5900.041.1864428
AATCGTT2950.041.1864422
ATTTACG556.184564E-1140.9090921
GGTCTAA5900.040.80508432
Produced by FastQC (version 0.11.5)