##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545196_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1516849
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.030289765164497	31.0	31.0	33.0	30.0	34.0
2	31.320949547384085	31.0	31.0	34.0	30.0	34.0
3	31.45424297342715	31.0	31.0	34.0	30.0	34.0
4	35.24034758898216	37.0	35.0	37.0	33.0	37.0
5	35.1041876943585	37.0	35.0	37.0	32.0	37.0
6	35.170209427569915	37.0	35.0	37.0	32.0	37.0
7	35.72559364841194	37.0	35.0	37.0	35.0	37.0
8	35.71483252452947	37.0	35.0	37.0	35.0	37.0
9	37.44910073448313	39.0	37.0	39.0	35.0	39.0
10	36.58663452987081	39.0	35.0	39.0	32.0	39.0
11	36.31714033499709	38.0	35.0	39.0	32.0	39.0
12	35.82354934472713	37.0	35.0	39.0	31.0	39.0
13	35.61878473071479	37.0	35.0	39.0	30.0	39.0
14	36.52422752693248	38.0	35.0	40.0	30.0	41.0
15	36.74680604331743	38.0	35.0	40.0	31.0	41.0
16	36.8207606689921	38.0	35.0	41.0	31.0	41.0
17	36.756773416470594	38.0	35.0	40.0	31.0	41.0
18	36.6663735150961	38.0	35.0	40.0	31.0	41.0
19	36.61012928775376	38.0	35.0	40.0	31.0	41.0
20	36.50421366925779	38.0	35.0	40.0	31.0	41.0
21	36.39787019011121	38.0	35.0	40.0	30.0	41.0
22	36.34747229289138	38.0	35.0	40.0	30.0	41.0
23	36.29353943602824	38.0	35.0	40.0	30.0	41.0
24	36.2095521703215	38.0	34.0	40.0	30.0	41.0
25	36.0886680216686	38.0	34.0	40.0	30.0	41.0
26	35.978614219345495	38.0	34.0	40.0	30.0	41.0
27	35.8880211543799	38.0	34.0	40.0	30.0	41.0
28	35.896095788044825	38.0	34.0	40.0	30.0	41.0
29	35.86169223172511	38.0	34.0	40.0	30.0	41.0
30	35.776995600748656	38.0	34.0	40.0	29.0	41.0
31	35.655264960454204	38.0	34.0	40.0	29.0	41.0
32	35.5264808824082	38.0	34.0	40.0	29.0	41.0
33	35.34707475826532	38.0	34.0	40.0	27.0	41.0
34	35.17393623228153	38.0	34.0	40.0	27.0	41.0
35	35.04561627426329	37.0	34.0	40.0	26.0	41.0
36	34.88853208196729	37.0	33.0	40.0	25.0	41.0
37	34.842020530718614	37.0	33.0	40.0	25.0	41.0
38	34.7913035509797	37.0	33.0	40.0	25.0	41.0
39	34.74538203868678	37.0	33.0	40.0	25.0	41.0
40	34.60438909871714	37.0	33.0	40.0	24.0	41.0
41	34.53322908213013	37.0	33.0	40.0	24.0	41.0
42	34.4826828510946	37.0	33.0	40.0	24.0	41.0
43	34.44295509968362	37.0	33.0	40.0	24.0	41.0
44	34.330562897163794	36.0	33.0	40.0	24.0	41.0
45	34.26244537195199	36.0	33.0	40.0	24.0	41.0
46	34.217084231851686	36.0	33.0	40.0	24.0	41.0
47	34.101788642112695	36.0	33.0	40.0	23.0	41.0
48	34.038337369111886	36.0	33.0	40.0	23.0	41.0
49	33.993171370386904	36.0	33.0	40.0	24.0	41.0
50	33.94220584909902	36.0	33.0	40.0	24.0	41.0
51	33.77792779637261	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	12.0
15	45.0
16	156.0
17	376.0
18	869.0
19	1611.0
20	2918.0
21	4571.0
22	6950.0
23	9854.0
24	14037.0
25	19356.0
26	25072.0
27	30144.0
28	33567.0
29	38623.0
30	46427.0
31	56871.0
32	69597.0
33	87086.0
34	128412.0
35	165435.0
36	123924.0
37	152068.0
38	206039.0
39	292680.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.25957362928017	23.481243024190277	28.728238605161096	13.530944741368456
2	31.307664770850625	25.387497371195156	30.48859840366444	12.816239454289782
3	29.46245802977093	25.289926683539367	31.94550017833021	13.3021151083595
4	26.039375046560338	27.637095056923926	31.52185880071121	14.801671095804526
5	22.393395782968508	33.1013172702095	30.53039557661969	13.974891370202307
6	20.3552891553477	42.15482226642204	27.531811010852103	9.958077567378163
7	89.18712409738873	3.436334137412491	6.018397348714341	1.3581444164844358
8	90.72584021217669	2.428916787366442	5.574318867599873	1.2709241328569951
9	86.41255655638761	4.260147186700851	6.889677219024438	2.4376190378870937
10	40.106892643895335	31.227168953534594	14.267801211590605	14.398137190979456
11	34.165892583902554	22.48147310641995	25.59430767334125	17.758326636336246
12	34.17439705600228	22.255676075865168	26.112684914582797	17.457241953549758
13	25.047714044047893	30.52874742311199	26.391288783524264	18.032249749315852
14	19.677898063683333	33.10876692406429	27.893481816581613	19.31985319567076
15	18.332543318418644	25.373850660151405	36.95034904594986	19.34325697548009
16	21.459090522523997	21.873304462078956	35.91036418259168	20.757240832805373
17	21.71587283902353	21.711653566043818	29.020818815847854	27.5516547790848
18	23.211736962611308	24.488396669675097	31.62463765345133	20.675228714262264
19	26.66099262352416	25.622721839813984	25.566882398973135	22.149403137688722
20	27.851355012924824	24.489517414060334	26.65578445843983	21.003343114575017
21	23.626412385148424	27.334164442208813	29.43674683505082	19.602676337591944
22	23.70954524807677	23.480122279805045	28.035091165963127	24.775241306155063
23	21.305944098588586	28.20583986936076	27.28887318381724	23.19934284823341
24	20.50045851630584	25.67308941100927	34.05751000923625	19.768942063448634
25	21.229733480392575	26.555510799031413	30.626515889188706	21.588239831387305
26	20.649913076384006	29.83698443286049	26.681495653159935	22.83160683759557
27	19.665372097024818	30.116313489345348	28.742742356028845	21.475572057600985
28	18.901090352434554	27.515065771213877	32.58821412019258	20.995629756158984
29	19.56958141515734	26.041616535330807	31.93640237096771	22.45239967854414
30	20.878544930972033	28.656840595207566	30.53402151433663	19.93059295948377
31	25.972723718708984	26.88652594951772	27.261184204887893	19.879566126885408
32	26.823632411663915	26.690066051399974	26.5529396795594	19.93336185737671
33	25.815423947934175	26.661058549664467	26.267413565885594	21.256103936515764
34	20.631453757097773	26.68400084649164	28.915732548196953	23.76881284821363
35	21.91233273714127	26.261612065538493	29.151088869096398	22.67496632822384
36	26.429657797183502	27.293092456796952	25.623381101217063	20.653868644802483
37	21.290781086317757	30.756654090156633	27.338054084486984	20.614510739038625
38	21.728992140944815	30.61122102463726	26.09936783424059	21.56041900017734
39	21.456387550771368	29.44933872784964	25.93751915978453	23.156754561594465
40	23.598525627798153	26.795416023612106	26.997281865235102	22.60877648335464
41	20.916716166210346	25.0684807782449	27.988019901783233	26.026783153761514
42	21.47425353479483	26.665607453345718	25.70427247537494	26.155866536484513
43	20.931483621639334	27.153856448466524	26.495320232930236	25.419339696963906
44	21.752461846894448	27.86981433221105	27.723524226867667	22.654199594026828
45	20.045963705022714	31.2917106448961	25.415450054685735	23.246875595395455
46	22.152172035581657	29.7584663997537	26.84288284463384	21.2464787200308
47	21.604391735762757	28.168987156928605	27.779231815427902	22.447389291880736
48	21.296318882103623	27.866847655897192	28.73173269059742	22.105100771401766
49	21.59740356489011	26.54239149711013	29.278655950592313	22.581548987407448
50	21.338181981199185	29.31122346390445	27.965011678815756	21.385582876080612
51	20.91091466586325	29.534383448846917	26.903139336875327	22.651562548414507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	673.0
1	1157.5
2	1642.0
3	7398.0
4	13154.0
5	9441.5
6	5729.0
7	5942.5
8	6156.0
9	6500.0
10	6844.0
11	7022.5
12	7201.0
13	7075.5
14	6950.0
15	6551.0
16	6152.0
17	5772.5
18	5393.0
19	5281.0
20	5169.0
21	4980.5
22	4792.0
23	5096.5
24	5401.0
25	6789.0
26	10176.0
27	12175.0
28	15076.5
29	17978.0
30	20529.5
31	23081.0
32	28464.0
33	33847.0
34	39272.0
35	44697.0
36	48335.0
37	51973.0
38	58029.5
39	64086.0
40	76445.5
41	88805.0
42	100915.0
43	113025.0
44	115218.0
45	117411.0
46	114606.5
47	111802.0
48	116446.0
49	121090.0
50	118684.5
51	116279.0
52	110063.0
53	103847.0
54	96528.0
55	89209.0
56	84083.5
57	78958.0
58	72908.5
59	66859.0
60	60069.0
61	53279.0
62	47058.0
63	40837.0
64	34741.0
65	28645.0
66	24066.0
67	19487.0
68	15265.5
69	11044.0
70	10549.5
71	10055.0
72	8354.5
73	6654.0
74	5232.5
75	3096.5
76	2382.0
77	1633.0
78	884.0
79	811.0
80	738.0
81	521.0
82	304.0
83	201.5
84	99.0
85	70.0
86	41.0
87	33.5
88	26.0
89	17.0
90	8.0
91	4.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1516849.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.002993258681197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45640411624926	15.293569361203394
2	7.299050798274576	2.920057278253159
3	2.9941057793862615	1.7967323316252541
4	1.6957986344949358	1.3568419461553194
5	1.1803748120923407	1.180551470450009
6	0.870603214879591	1.0448802142933584
7	0.682381050879212	0.955476449241207
8	0.5827481629823698	0.9325366060516156
9	0.4604101270801114	0.8288622601390819
>10	5.345279031585074	23.993903078150606
>50	1.4843130288821844	21.39483588814693
>100	0.942247389092425	26.787183777820694
>500	0.004630208300203927	0.6196807457605882
>1k	0.001322916657201122	0.3799985271323603
>5k	3.307291643002805E-4	0.5148900655764556
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7783	0.5131031500169101	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1938	0.12776485991684078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.592614030796737E-5	0.0	0.0	0.019909694373006145	0.0
2	6.592614030796737E-5	0.0	0.0	0.07443061240769516	0.0
3	6.592614030796737E-5	0.0	0.0	0.1066684950182912	0.0
4	6.592614030796737E-5	0.0	0.0	0.14694936674645928	0.0
5	6.592614030796737E-5	0.0	0.0	0.25038748088966006	0.0
6	6.592614030796737E-5	0.0	0.0	0.36945009028584913	0.0
7	6.592614030796737E-5	0.0	0.0	0.4471110835686347	0.0
8	6.592614030796737E-5	0.0	0.0	0.6484495160691671	0.0
9	6.592614030796737E-5	0.0	0.0	0.7418668568855569	0.0
10	6.592614030796737E-5	0.0	0.0	0.901276264150222	0.0
11	6.592614030796737E-5	0.0	0.0	1.050664898088076	0.0
12	6.592614030796737E-5	0.0	0.0	1.1833742185280143	0.0
13	6.592614030796737E-5	0.0	0.0	1.2400706991928663	0.0
14	6.592614030796737E-5	0.0	0.0	1.2681552349640604	0.0
15	6.592614030796737E-5	0.0	0.0	1.3007886744165043	0.0
16	1.3185228061593474E-4	0.0	0.0	1.378185963138058	0.0
17	1.3185228061593474E-4	0.0	0.0	1.4652084683445747	0.0
18	1.3185228061593474E-4	0.0	0.0	1.5891496121235535	0.0
19	1.3185228061593474E-4	0.0	0.0	1.6440001608597823	0.0
20	1.3185228061593474E-4	0.0	0.0	1.7097944488871337	0.0
21	1.3185228061593474E-4	0.0	0.0	1.7920043458511692	0.0
22	1.3185228061593474E-4	0.0	0.0	1.8783016635142984	0.0
23	1.3185228061593474E-4	0.0	0.0	1.9733012316980794	0.0
24	1.3185228061593474E-4	0.0	0.0	2.0477318441057744	0.0
25	1.3185228061593474E-4	0.0	0.0	2.113789836694358	0.0
26	1.3185228061593474E-4	0.0	0.0	2.1793863463007854	0.0
27	1.3185228061593474E-4	0.0	0.0	2.26258513536944	0.0
28	1.3185228061593474E-4	0.0	0.0	2.3326646225168095	0.0
29	1.3185228061593474E-4	0.0	0.0	2.417050082111008	0.0
30	1.9777842092390211E-4	0.0	0.0	2.5233230202874513	0.0
31	2.637045612318695E-4	0.0	0.0	2.612455161983823	0.0
32	2.637045612318695E-4	0.0	0.0	2.6919620871952317	0.0
33	2.637045612318695E-4	0.0	0.0	2.7802371890676	0.0
34	2.637045612318695E-4	0.0	0.0	2.875302683391689	0.0
35	2.637045612318695E-4	0.0	0.0	2.990343798229092	0.0
36	2.637045612318695E-4	0.0	0.0	3.087123372201188	0.0
37	2.637045612318695E-4	0.0	0.0	3.186210361084063	0.0
38	2.637045612318695E-4	0.0	0.0	3.308173720653803	0.0
39	2.637045612318695E-4	0.0	0.0	3.505490658595549	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	7.0339994E-4	45.000004	1
CGTTACA	20	7.0339994E-4	45.000004	39
GACGATA	20	7.0339994E-4	45.000004	9
TAGTACG	75	0.0	45.000004	1
TCTATCG	20	7.0339994E-4	45.000004	32
AACGTTG	45	3.8562575E-10	45.0	1
CGACACG	25	3.8913975E-5	45.0	26
CGCATTA	30	2.1659635E-6	44.999996	16
GGCGTAG	130	0.0	43.26923	1
CGTTTTT	5310	0.0	43.008472	1
CTAGACG	80	0.0	42.187504	1
ATACTCG	80	0.0	42.187504	44
CGGTCTA	575	0.0	41.869564	31
TAGGGTA	1010	0.0	41.658413	5
ACGGGAT	660	0.0	41.590908	5
GCGTAAG	65	0.0	41.53846	1
CGACGGT	590	0.0	41.18644	28
AATCGTT	295	0.0	41.18644	22
ATTTACG	55	6.184564E-11	40.909092	1
GGTCTAA	590	0.0	40.805084	32
>>END_MODULE