FastQCFastQC Report
Thu 26 May 2016
SRR1545195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545195_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1816103
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64540.3553763195149174No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22280.12268026648268299No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA600.045.00000427
AACCGGT207.0343685E-445.041
AGGCGTA253.8917027E-545.013
TCTAGCG406.8193913E-945.01
TACGCTT253.8917027E-545.023
GTTCGAC253.8917027E-545.041
TATTACG207.0343685E-445.01
TCCCGTA351.2124474E-745.041
TCGTAAT207.0343685E-445.032
ACGTATC207.0343685E-445.039
GTCCAAT253.8917027E-545.010
TAGTCCG253.8917027E-545.025
TACCGTA207.0343685E-445.035
CTTACGC253.8917027E-545.045
ACGGTAC207.0343685E-445.013
TCACGAC5250.043.71428325
CGGTCTA5250.043.71428331
TACGGGT1300.043.2692344
TAACGGG7700.042.9545443
CGAATAT1050.042.85714314