##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545195_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1816103
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07628256767375	31.0	31.0	33.0	30.0	34.0
2	31.35284782856479	31.0	31.0	34.0	30.0	34.0
3	31.479389109538392	31.0	31.0	34.0	30.0	34.0
4	35.24717320548449	37.0	35.0	37.0	33.0	37.0
5	35.10103226524046	37.0	35.0	37.0	32.0	37.0
6	35.15400227850513	37.0	35.0	37.0	32.0	37.0
7	35.76448472360874	37.0	35.0	37.0	35.0	37.0
8	35.76169358235739	37.0	35.0	37.0	35.0	37.0
9	37.487056075564	39.0	37.0	39.0	35.0	39.0
10	36.627812959947754	39.0	35.0	39.0	32.0	39.0
11	36.29405490767869	38.0	35.0	39.0	32.0	39.0
12	35.58922814399844	37.0	35.0	39.0	30.0	39.0
13	35.29184633250427	37.0	35.0	39.0	30.0	39.0
14	36.23937684151174	38.0	35.0	40.0	30.0	41.0
15	36.51696682401824	38.0	35.0	40.0	31.0	41.0
16	36.645391808724504	38.0	35.0	40.0	31.0	41.0
17	36.570017229199	38.0	35.0	40.0	31.0	41.0
18	36.52737317211634	38.0	35.0	40.0	31.0	41.0
19	36.46416530339964	38.0	35.0	40.0	31.0	41.0
20	36.345984231070595	38.0	35.0	40.0	31.0	41.0
21	36.226811474899826	38.0	34.0	40.0	30.0	41.0
22	36.131516769698635	38.0	34.0	40.0	30.0	41.0
23	36.07995306433611	38.0	34.0	40.0	30.0	41.0
24	36.00041572531954	37.0	34.0	40.0	30.0	41.0
25	35.90629331045651	37.0	34.0	40.0	30.0	41.0
26	35.73963205831387	37.0	34.0	40.0	30.0	41.0
27	35.669685585013625	37.0	34.0	40.0	29.0	41.0
28	35.65113432442984	37.0	34.0	40.0	29.0	41.0
29	35.64098291781909	37.0	34.0	40.0	29.0	41.0
30	35.58705425848644	37.0	34.0	40.0	29.0	41.0
31	35.456830917629674	37.0	34.0	40.0	29.0	41.0
32	35.3008293031838	37.0	34.0	40.0	28.0	41.0
33	35.115146552811154	37.0	34.0	40.0	27.0	41.0
34	34.998740159561436	37.0	34.0	40.0	27.0	41.0
35	34.88374117547298	37.0	33.0	40.0	26.0	41.0
36	34.71396446126679	37.0	33.0	40.0	25.0	41.0
37	34.66037278722627	36.0	33.0	40.0	25.0	41.0
38	34.63526352855537	36.0	33.0	40.0	26.0	41.0
39	34.58855637593242	36.0	33.0	40.0	25.0	41.0
40	34.485899753483146	36.0	33.0	40.0	24.0	41.0
41	34.42753797554434	36.0	33.0	40.0	24.0	41.0
42	34.33774626218887	36.0	33.0	40.0	24.0	41.0
43	34.26367667472605	36.0	33.0	40.0	24.0	41.0
44	34.13934121577906	35.0	33.0	40.0	24.0	41.0
45	34.05799726116856	35.0	33.0	40.0	24.0	41.0
46	34.004485428414576	35.0	33.0	39.0	24.0	41.0
47	33.90341186595694	35.0	33.0	39.0	23.0	41.0
48	33.840011276893435	35.0	33.0	39.0	23.0	41.0
49	33.81871622920065	35.0	33.0	39.0	24.0	41.0
50	33.755695574535146	35.0	33.0	39.0	24.0	41.0
51	33.573116172375684	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	7.0
14	14.0
15	55.0
16	165.0
17	422.0
18	1007.0
19	1920.0
20	3301.0
21	5329.0
22	8122.0
23	11626.0
24	16379.0
25	22666.0
26	29640.0
27	35815.0
28	41544.0
29	48465.0
30	57988.0
31	70646.0
32	87049.0
33	109616.0
34	168505.0
35	231771.0
36	143093.0
37	175091.0
38	229165.0
39	316495.0
40	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.60332536205271	23.768640875545056	29.407252782468834	13.220780979933409
2	29.968234180550336	25.624207437573748	30.962120540520004	13.445437841355915
3	28.601020977334436	26.151600432354332	31.673203557287223	13.574175033024009
4	26.055240258950068	28.552895953588536	30.620289708237912	14.771574079223479
5	22.87700642529636	33.32393592213658	29.648098153023263	14.150959499543802
6	19.999196080839027	42.568235391935374	26.471130767362865	10.96143775986274
7	89.55455720297803	3.3844996676950587	5.551887750859946	1.5090553784669702
8	90.60521347082187	2.580084940116282	5.040573139298817	1.7741284497630367
9	86.59888783841005	4.546933736687842	6.356247415482493	2.49793100941962
10	45.891339863432854	29.336662072580683	12.771742571869545	12.000255492116912
11	40.169748081468946	22.36530637304162	20.95635544900262	16.50859009648682
12	36.27580594272462	22.00012884731758	24.390632029130508	17.3334331808273
13	22.937410488281778	35.12978063468867	24.699700402455147	17.233108474574404
14	18.207832925775687	36.547156191031014	27.457253250503967	17.787757632689335
15	16.431336768894717	22.83306618622402	41.89349392628061	18.842103118600654
16	18.99137879294291	18.76969533115688	39.292595188709015	22.9463306871912
17	18.4717496749909	20.264929907609865	28.48891279844811	32.77440761895113
18	22.50885549993585	23.07424193451583	32.60250106959792	21.814401495950396
19	27.746058455935596	24.754212729123847	25.28022914999865	22.21949966494191
20	29.72920588755153	23.843085992369375	24.800300423489197	21.627407696589895
21	23.043461741982696	27.399106768724018	27.083706155432814	22.473725333860468
22	23.19136084241918	23.55229852051343	25.1078820969956	28.14845854007179
23	20.82569105386644	27.440459048853505	25.586654501424206	26.147195395855853
24	20.838355533799568	23.848977728686094	35.75661732842245	19.556049409091887
25	19.767325972150257	25.791048194953703	32.22256667160398	22.219059161292062
26	19.67762841644995	30.613241649840344	26.16195226812576	23.547177665583945
27	19.80906369297336	30.016469330208693	27.235734977586624	22.938731999231322
28	18.52780376443407	26.609008409765305	34.40553757138224	20.45765025441839
29	19.250505064966028	23.854539087265426	33.54429787297306	23.35065797479548
30	21.1381733304774	27.889001890311288	29.958598163209903	21.014226616001405
31	29.040863871707717	25.81681765846981	24.236290562814993	20.906027907007477
32	29.631854580935112	24.758177261972474	25.28353292737251	20.3264352297199
33	27.187499827928264	25.916096168554315	24.455110750877015	22.441293252640406
34	20.362556529007442	26.39007809579082	27.210736395457747	26.03662897974399
35	21.833398215850092	24.36899228733172	29.33264247677582	24.464967020042366
36	28.915375394457254	25.876616028936684	25.28887403412692	19.919134542479146
37	21.51381281788533	31.23258978152671	26.244766954297194	21.008830446290766
38	21.03845431674305	30.94659278686286	25.426090921054588	22.588861975339505
39	20.773050867709596	29.848582376660353	26.574373810296002	22.80399294533405
40	23.547122602627717	25.73163526518044	26.136843560084422	24.584398572107418
41	20.215152995177036	23.269715429135903	27.447452044294845	29.067679531392216
42	21.849862039763163	25.81009997780963	24.379178934234456	27.96085904819275
43	21.995888999687793	25.34817683798771	26.431099998182923	26.22483416414157
44	21.10634694177588	26.83074693450757	28.651513708198266	23.411392415518286
45	18.886593987235305	32.74549956692985	24.488919406002854	23.87898703983199
46	22.319879434151034	28.746056804046905	27.360012069800003	21.57405169200205
47	20.622949249023872	27.199448489430388	27.509893436660803	24.667708824884933
48	21.196540064082267	24.675527764669734	30.467545067653102	23.660387103594896
49	20.845568781065833	24.269163147684907	31.57188771782217	23.31338035342709
50	20.48716399895821	28.864772537680956	28.479386907020142	22.168676556340692
51	19.510677533157537	29.21161409898007	26.727834269311817	24.54987409855058
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	851.0
1	1447.5
2	2044.0
3	6791.0
4	11538.0
5	8559.5
6	5581.0
7	5850.0
8	6119.0
9	6385.0
10	6651.0
11	6760.0
12	6869.0
13	6763.0
14	6657.0
15	6313.5
16	5970.0
17	5622.0
18	5274.0
19	5720.5
20	6167.0
21	5781.5
22	5396.0
23	5399.5
24	5403.0
25	6326.0
26	8821.0
27	10393.0
28	15375.5
29	20358.0
30	24814.5
31	29271.0
32	32639.0
33	36007.0
34	39390.0
35	42773.0
36	46869.5
37	50966.0
38	57893.5
39	64821.0
40	85394.0
41	105967.0
42	123846.5
43	141726.0
44	148617.0
45	155508.0
46	152757.5
47	150007.0
48	150700.0
49	151393.0
50	145460.0
51	139527.0
52	130901.5
53	122276.0
54	108369.0
55	94462.0
56	95701.5
57	96941.0
58	87883.5
59	78826.0
60	73130.0
61	67434.0
62	58212.5
63	48991.0
64	43724.5
65	38458.0
66	33632.0
67	28806.0
68	26340.5
69	23875.0
70	19057.5
71	14240.0
72	11802.0
73	9364.0
74	7521.5
75	4538.5
76	3398.0
77	2395.0
78	1392.0
79	1082.0
80	772.0
81	622.5
82	473.0
83	330.5
84	188.0
85	107.5
86	27.0
87	19.0
88	11.0
89	6.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1816103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.84827928327227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09881539430448	14.117777481335422
2	8.04107070388844	2.8703855131907927
3	2.8391356815853452	1.5202105970411643
4	1.4354703084316645	1.0248269987093332
5	0.8784212108258345	0.7839153549584841
6	0.6177771041038748	0.6615754973314274
7	0.4779444596939089	0.5971340338956687
8	0.37239957194850737	0.5317353252086404
9	0.3093196738427994	0.4968741533901274
>10	3.3909109203236407	13.865925133798207
>50	1.0284737718949781	13.24656485887051
>100	1.498208777063869	48.414001529391626
>500	0.010816276237047562	1.2481082357226485
>1k	9.271093917469338E-4	0.2649775483653705
>5k	3.090364639156446E-4	0.35598773879061224
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6454	0.3553763195149174	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2228	0.12268026648268299	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04405036498480538	0.0
2	0.0	0.0	0.0	0.1492206113860282	0.0
3	0.0	0.0	0.0	0.21149681488329683	0.0
4	0.0	0.0	0.0	0.31022469540549186	0.0
5	0.0	0.0	0.0	0.5139576334602167	0.0
6	0.0	0.0	0.0	0.7440106645933628	0.0
7	0.0	0.0	0.0	0.8986274456900297	0.0
8	0.0	0.0	0.0	1.3080205252675647	0.0
9	0.0	0.0	0.0	1.5029984532815595	0.0
10	0.0	0.0	0.0	1.8376160382973874	0.0
11	0.0	0.0	0.0	2.1102877975533327	0.0
12	0.0	0.0	0.0	2.374094420856086	0.0
13	0.0	0.0	0.0	2.476181141708372	0.0
14	0.0	0.0	0.0	2.5175884847940893	0.0
15	0.0	0.0	0.0	2.5737527001497162	0.0
16	0.0	0.0	0.0	2.7006728142621865	0.0
17	0.0	0.0	0.0	2.85292188824092	0.0
18	0.0	0.0	0.0	3.0853426264919994	0.0
19	0.0	0.0	0.0	3.177628141135167	0.0
20	0.0	0.0	0.0	3.276851588263441	0.0
21	0.0	0.0	0.0	3.3979900919716557	0.0
22	0.0	0.0	0.0	3.5239741358281993	0.0
23	0.0	0.0	0.0	3.678425728056173	0.0
24	0.0	0.0	0.0	3.7886067034744175	0.0
25	0.0	0.0	0.0	3.8713112637333897	0.0
26	0.0	0.0	0.0	3.9607335046525445	0.0
27	0.0	0.0	0.0	4.0501557455717	0.0
28	5.506295623100672E-5	0.0	0.0	4.144038085945565	0.0
29	5.506295623100672E-5	0.0	0.0	4.243922288548612	0.0
30	5.506295623100672E-5	0.0	0.0	4.372163913610627	0.0
31	5.506295623100672E-5	0.0	0.0	4.486419547789966	0.0
32	5.506295623100672E-5	0.0	0.0	4.587680324298787	0.0
33	5.506295623100672E-5	0.0	0.0	4.695768907380253	0.0
34	1.1012591246201344E-4	0.0	0.0	4.804903686630109	0.0
35	1.1012591246201344E-4	0.0	0.0	4.957703390171152	0.0
36	1.1012591246201344E-4	0.0	0.0	5.072784968693957	0.0
37	1.1012591246201344E-4	0.0	0.0	5.183296321849586	0.0
38	1.1012591246201344E-4	0.0	0.0	5.304159510776646	0.0
39	1.1012591246201344E-4	0.0	0.0	5.426619525434406	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAA	60	0.0	45.000004	27
AACCGGT	20	7.0343685E-4	45.0	41
AGGCGTA	25	3.8917027E-5	45.0	13
TCTAGCG	40	6.8193913E-9	45.0	1
TACGCTT	25	3.8917027E-5	45.0	23
GTTCGAC	25	3.8917027E-5	45.0	41
TATTACG	20	7.0343685E-4	45.0	1
TCCCGTA	35	1.2124474E-7	45.0	41
TCGTAAT	20	7.0343685E-4	45.0	32
ACGTATC	20	7.0343685E-4	45.0	39
GTCCAAT	25	3.8917027E-5	45.0	10
TAGTCCG	25	3.8917027E-5	45.0	25
TACCGTA	20	7.0343685E-4	45.0	35
CTTACGC	25	3.8917027E-5	45.0	45
ACGGTAC	20	7.0343685E-4	45.0	13
TCACGAC	525	0.0	43.714283	25
CGGTCTA	525	0.0	43.714283	31
TACGGGT	130	0.0	43.269234	4
TAACGGG	770	0.0	42.954544	3
CGAATAT	105	0.0	42.857143	14
>>END_MODULE