FastQCFastQC Report
Thu 26 May 2016
SRR1545193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545193_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1328140
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43500.32752571265077474No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14670.11045522309395095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAATA302.165758E-645.00000432
GCGCTAA302.165758E-645.00000422
TACAACG302.165758E-645.00000433
CGTGCTA207.033684E-445.024
CAACGAT207.033684E-445.021
CGCATCG406.8157533E-945.021
CGATTCA502.1827873E-1145.010
CGCGAAA253.8911352E-545.024
ACTTACG207.033684E-445.01
ATCGTAG502.1827873E-1145.01
GCTTAAC207.033684E-445.011
GCGATTC1800.045.09
CGTACGA253.8911352E-545.025
TCCGTCG207.033684E-445.016
CATCCGA253.8911352E-545.036
GTGCGAT253.8911352E-545.018
CTTACCG253.8911352E-545.01
CTCACGA5300.042.4528324
CGTTTTT38750.042.3290331
CGGTCTA5250.042.031
Produced by FastQC (version 0.11.5)