##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545193_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1328140
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02440405378951	31.0	31.0	33.0	30.0	34.0
2	31.290357191259957	31.0	31.0	34.0	28.0	34.0
3	31.405966991431626	31.0	31.0	34.0	28.0	34.0
4	35.18099070881082	37.0	35.0	37.0	32.0	37.0
5	35.021442769587544	35.0	35.0	37.0	32.0	37.0
6	35.09549520381888	37.0	35.0	37.0	32.0	37.0
7	35.729711476199796	37.0	35.0	37.0	35.0	37.0
8	35.74108603008719	37.0	35.0	37.0	35.0	37.0
9	37.430766334874335	39.0	37.0	39.0	35.0	39.0
10	36.5758112849549	39.0	35.0	39.0	32.0	39.0
11	36.25284683843571	38.0	35.0	39.0	31.0	39.0
12	35.51530335657385	37.0	35.0	39.0	30.0	39.0
13	35.20195386028581	37.0	34.0	39.0	30.0	39.0
14	36.1337554775852	38.0	35.0	40.0	30.0	41.0
15	36.43366663152981	38.0	35.0	40.0	30.0	41.0
16	36.55589169816435	38.0	35.0	40.0	31.0	41.0
17	36.49142936738597	38.0	35.0	40.0	31.0	41.0
18	36.40540680952309	38.0	35.0	40.0	31.0	41.0
19	36.35386329754393	38.0	35.0	40.0	30.0	41.0
20	36.238602858132424	38.0	34.0	40.0	30.0	41.0
21	36.08482388904784	38.0	34.0	40.0	30.0	41.0
22	35.981538090863914	38.0	34.0	40.0	30.0	41.0
23	35.90680425256374	37.0	34.0	40.0	30.0	41.0
24	35.840380532172816	37.0	34.0	40.0	30.0	41.0
25	35.72559519327782	37.0	34.0	40.0	29.0	41.0
26	35.55972713719939	37.0	34.0	40.0	29.0	41.0
27	35.48785971358441	37.0	34.0	40.0	29.0	41.0
28	35.492486484858524	37.0	34.0	40.0	29.0	41.0
29	35.47259852124023	37.0	34.0	40.0	29.0	41.0
30	35.426901531465056	37.0	34.0	40.0	29.0	41.0
31	35.2842501543512	37.0	34.0	40.0	28.0	41.0
32	35.14178701040553	37.0	34.0	40.0	27.0	41.0
33	34.951923742978906	37.0	33.0	40.0	27.0	41.0
34	34.80640820997787	37.0	34.0	40.0	26.0	41.0
35	34.67368726188504	37.0	33.0	40.0	25.0	41.0
36	34.52690830785911	36.0	33.0	40.0	24.0	41.0
37	34.455031096119384	36.0	33.0	40.0	24.0	41.0
38	34.40618534190673	36.0	33.0	40.0	24.0	41.0
39	34.37756637101511	36.0	33.0	40.0	24.0	41.0
40	34.22307889228545	36.0	33.0	40.0	23.0	41.0
41	34.19933440751728	36.0	33.0	40.0	23.0	41.0
42	34.124285843359885	36.0	33.0	40.0	23.0	41.0
43	34.05309078862168	35.0	33.0	40.0	23.0	41.0
44	33.928112247202854	35.0	33.0	40.0	23.0	41.0
45	33.841101841673314	35.0	33.0	40.0	23.0	41.0
46	33.777422560874605	35.0	33.0	39.0	23.0	41.0
47	33.67895026126764	35.0	32.0	39.0	23.0	41.0
48	33.622856777146986	35.0	32.0	39.0	23.0	41.0
49	33.60269474603581	35.0	32.0	39.0	23.0	41.0
50	33.53060294848434	35.0	32.0	39.0	23.0	41.0
51	33.36820892375804	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	5.0
14	11.0
15	41.0
16	141.0
17	432.0
18	934.0
19	1774.0
20	2944.0
21	4940.0
22	7064.0
23	9957.0
24	13716.0
25	18596.0
26	23364.0
27	27562.0
28	31715.0
29	36271.0
30	43037.0
31	52547.0
32	64047.0
33	80827.0
34	123372.0
35	165224.0
36	103851.0
37	125492.0
38	164748.0
39	225428.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.88653304621501	23.2989745056997	28.512054452090897	13.3024379959944
2	30.919631966509552	25.387986206273432	30.70293794328911	12.989443883927898
3	28.672956164259794	26.621365217522246	31.64003794780671	13.065640670411252
4	27.101284503139727	28.754047013116086	29.79964461577846	14.345023867965725
5	22.732618549249324	32.99817790293192	30.334302106705618	13.934901441113134
6	20.50024846778201	43.24551628593371	26.06901380878522	10.18522143749906
7	88.59713584411281	4.020886352342373	5.893354616230217	1.4886231873145903
8	90.30207658831148	2.946376135046004	5.274594545755718	1.4769527308868042
9	85.86813137169274	5.307422410288072	6.476877437619528	2.3475687803996568
10	44.35247790142605	32.524357371963795	12.17236134744831	10.950803379161837
11	41.46889635128827	22.49371301218245	20.92015901938049	15.117231617148793
12	38.018657671631004	22.004909121026397	23.777312632704383	16.199120574638215
13	23.382550032376102	35.926709533633506	24.623458370352523	16.06728206363787
14	18.04629030072131	37.04383574020811	27.53610312165886	17.37377083741172
15	16.37395154125318	23.99317842998479	41.211167497402386	18.421702531359646
16	19.35526375231527	19.75318866986914	40.18371557215354	20.707832005662052
17	19.33523574321984	20.59669914316262	28.18814281626937	31.879922297348173
18	22.57487915430602	23.253422078997694	31.783396328700285	22.388302437995993
19	27.88764738657069	24.906109295706777	24.96423569804388	22.242007619678645
20	30.026503230081165	23.690951255138764	25.24477841191441	21.03776710286566
21	24.076528076859365	27.236285331365668	27.579999096480794	21.107187495294173
22	23.891607812429413	24.91348803589983	24.27936813890102	26.915536012769735
23	21.470477509901066	28.8071287665457	25.167753399491016	24.55464032406222
24	21.11065098558887	24.567891939102807	34.56472962187721	19.756727453431115
25	20.279187435059555	25.86060204496514	32.23357477374373	21.62663574623157
26	19.782251871037694	32.32806782417516	26.05719276582288	21.832487538964266
27	19.682111825560558	31.594485521104705	27.726369208065414	20.997033445269324
28	18.367190205851795	27.6038670622073	34.5456051319891	19.48333759995181
29	19.237956841899198	24.50697968587649	33.83958016474167	22.415483307482646
30	21.43998373665427	28.753444666977877	29.392458626349633	20.414112970018223
31	27.77342750011294	26.869381239929524	25.03674311443071	20.320448145526825
32	29.013357025614773	25.900507476621442	26.174876142575332	18.91125935518846
33	27.872513439848206	27.04391103347539	24.213637116569036	20.86993841010737
34	21.147017633683195	28.01722709955276	26.653741322450948	24.1820139443131
35	21.824732332434834	26.790323309289683	28.66294215971208	22.722002198563406
36	28.682593702471127	26.159742195852846	25.28618970891623	19.8714743927598
37	22.192464649811015	32.265047359465115	26.006219223877004	19.536268766846867
38	21.932702877708678	31.30859698525758	24.315734786995346	22.4429653500384
39	22.255786287590162	30.156308822865057	25.338142063336694	22.24976282620808
40	23.544882316623248	26.64018853434126	26.163431565949374	23.65149758308612
41	19.928170223018657	25.004216422967456	26.81818181818182	28.249431535832066
42	22.447708825876788	26.25114822232596	24.663966148147033	26.63717680365022
43	22.1334347282666	27.24916048006987	25.259987651904165	25.357417139759363
44	21.467089312873643	29.027587453129943	26.98435405906004	22.52096917493638
45	20.03937837878537	33.49172526992636	23.95078832050838	22.51810803077989
46	22.430316081136027	30.05383468610237	26.577092776363937	20.93875645639767
47	22.215278509795656	28.276988871655096	25.931904769075548	23.5758278494737
48	21.80447844353758	26.258451669251738	29.03571912599575	22.901350761214932
49	21.714051229539056	25.933711807490173	29.425060611080156	22.927176351890612
50	20.895688707515774	31.43012031864111	26.462948183173463	21.211242790669658
51	20.47841342027196	31.086933606396915	25.170463957112954	23.26418901621817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	719.0
1	1189.5
2	1660.0
3	4891.0
4	8122.0
5	6213.5
6	4305.0
7	4550.5
8	4796.0
9	5105.5
10	5415.0
11	5528.0
12	5641.0
13	5654.0
14	5667.0
15	5347.5
16	5028.0
17	4785.5
18	4543.0
19	4536.0
20	4529.0
21	4725.5
22	4922.0
23	4973.5
24	5025.0
25	5410.5
26	8309.0
27	10822.0
28	13452.0
29	16082.0
30	18494.0
31	20906.0
32	22650.5
33	24395.0
34	29839.0
35	35283.0
36	38428.5
37	41574.0
38	46188.5
39	50803.0
40	63760.0
41	76717.0
42	95119.5
43	113522.0
44	114109.0
45	114696.0
46	114356.5
47	114017.0
48	106676.5
49	99336.0
50	96694.5
51	94053.0
52	87360.0
53	80667.0
54	77833.0
55	74999.0
56	70659.0
57	66319.0
58	61044.5
59	55770.0
60	53093.5
61	50417.0
62	42998.5
63	35580.0
64	31526.5
65	27473.0
66	24781.5
67	22090.0
68	17502.5
69	12915.0
70	11331.0
71	9747.0
72	8122.0
73	6497.0
74	5079.0
75	2869.0
76	2077.0
77	1482.0
78	887.0
79	639.5
80	392.0
81	279.0
82	166.0
83	120.5
84	75.0
85	50.5
86	26.0
87	15.0
88	4.0
89	4.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1328140.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.866802435806378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41026983750355	14.793510699624473
2	7.505677867485748	2.8321628294531624
3	2.9282486946813795	1.6574006881638448
4	1.4820518987738023	1.1184632149510816
5	0.9762955560870548	0.9209787687825093
6	0.6645601212059418	0.7522874708104826
7	0.5324596213955025	0.7032067337339246
8	0.4035188279662298	0.6090488005093599
9	0.3497963190137911	0.5939584239244948
>10	4.184221713235615	18.112703739645045
>50	1.2368201766015134	16.855760694810968
>100	1.3204756500448118	40.06599905590666
>500	0.004803185147177248	0.5452348171602628
>1k	8.005308578628748E-4	0.4392840625237187
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4350	0.32752571265077474	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1467	0.11045522309395095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024846778201093257	0.0
2	0.0	0.0	0.0	0.08222024786543587	0.0
3	0.0	0.0	0.0	0.11708103061424248	0.0
4	0.0	0.0	0.0	0.16805457256012168	0.0
5	0.0	0.0	0.0	0.28814733386540575	0.0
6	0.0	0.0	0.0	0.4207387775385125	0.0
7	0.0	0.0	0.0	0.5037872513439848	0.0
8	0.0	0.0	0.0	0.7304199858448658	0.0
9	0.0	0.0	0.0	0.8317647235984158	0.0
10	0.0	0.0	0.0	1.002831026849579	0.0
11	0.0	0.0	0.0	1.1555257728853885	0.0
12	0.0	0.0	0.0	1.2820184619091362	0.0
13	0.0	0.0	0.0	1.3445871670155254	0.0
14	0.0	0.0	0.0	1.366422214525577	0.0
15	0.0	0.0	0.0	1.4067794057855347	0.0
16	0.0	0.0	0.0	1.4797385817760176	0.0
17	7.529326727604018E-5	0.0	0.0	1.5681328775580887	0.0
18	7.529326727604018E-5	0.0	0.0	1.7069736624151068	0.0
19	7.529326727604018E-5	0.0	0.0	1.7641965455448974	0.0
20	7.529326727604018E-5	0.0	0.0	1.8277440631258752	0.0
21	7.529326727604018E-5	0.0	0.0	1.9077808062403059	0.0
22	7.529326727604018E-5	0.0	0.0	1.9891728281657055	0.0
23	7.529326727604018E-5	0.0	0.0	2.082084719984339	0.0
24	7.529326727604018E-5	0.0	0.0	2.155345069043926	0.0
25	7.529326727604018E-5	0.0	0.0	2.2145255771228936	0.0
26	7.529326727604018E-5	0.0	0.0	2.2760401764874185	0.0
27	7.529326727604018E-5	0.0	0.0	2.336425376842803	0.0
28	7.529326727604018E-5	0.0	0.0	2.4001234809583325	0.0
29	7.529326727604018E-5	0.0	0.0	2.4685650609122534	0.0
30	1.5058653455208036E-4	0.0	0.0	2.5549264384778714	0.0
31	1.5058653455208036E-4	0.0	0.0	2.6358667007996144	0.0
32	1.5058653455208036E-4	0.0	0.0	2.706416492237264	0.0
33	1.5058653455208036E-4	0.0	0.0	2.7841191440661377	0.0
34	1.5058653455208036E-4	0.0	0.0	2.865360579456985	0.0
35	1.5058653455208036E-4	0.0	0.0	2.9643712259249777	0.0
36	1.5058653455208036E-4	0.0	0.0	3.056680771605403	0.0
37	1.5058653455208036E-4	0.0	0.0	3.141084524221844	0.0
38	1.5058653455208036E-4	0.0	0.0	3.2259400364419415	0.0
39	1.5058653455208036E-4	0.0	0.0	3.3107955486620386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAATA	30	2.165758E-6	45.000004	32
GCGCTAA	30	2.165758E-6	45.000004	22
TACAACG	30	2.165758E-6	45.000004	33
CGTGCTA	20	7.033684E-4	45.0	24
CAACGAT	20	7.033684E-4	45.0	21
CGCATCG	40	6.8157533E-9	45.0	21
CGATTCA	50	2.1827873E-11	45.0	10
CGCGAAA	25	3.8911352E-5	45.0	24
ACTTACG	20	7.033684E-4	45.0	1
ATCGTAG	50	2.1827873E-11	45.0	1
GCTTAAC	20	7.033684E-4	45.0	11
GCGATTC	180	0.0	45.0	9
CGTACGA	25	3.8911352E-5	45.0	25
TCCGTCG	20	7.033684E-4	45.0	16
CATCCGA	25	3.8911352E-5	45.0	36
GTGCGAT	25	3.8911352E-5	45.0	18
CTTACCG	25	3.8911352E-5	45.0	1
CTCACGA	530	0.0	42.45283	24
CGTTTTT	3875	0.0	42.329033	1
CGGTCTA	525	0.0	42.0	31
>>END_MODULE