FastQCFastQC Report
Thu 26 May 2016
SRR1545192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545192_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1097465
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63530.5788795086859262No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34650.315727608625332No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA207.033146E-445.00000412
CGTGCCG406.8139343E-945.00000441
TAGCGCT207.033146E-445.00000430
TAGCGCG406.8139343E-945.0000041
ACGATTC207.033146E-445.00000435
CTAACGG406.8139343E-945.0000042
CCTCCGT207.033146E-445.00000438
TCTACGA406.8139343E-945.00000413
CCATCCG207.033146E-445.00000430
CTACGTC207.033146E-445.00000432
CGGATCT207.033146E-445.00000415
GGGCGTA406.8139343E-945.0000047
GTATGAC406.8139343E-945.00000416
TACGACT207.033146E-445.00000440
TATTCGA207.033146E-445.00000413
TCGCTGA207.033146E-445.00000436
TCGCTAA406.8139343E-945.00000443
AGCGCGA207.033146E-445.00000431
AGCGATC207.033146E-445.00000439
AACGACC207.033146E-445.00000431