##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545192_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1097465
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.036144205054374	31.0	31.0	33.0	28.0	34.0
2	31.31685839639533	31.0	31.0	34.0	28.0	34.0
3	31.412371237351532	31.0	31.0	34.0	28.0	34.0
4	35.17058493892744	37.0	35.0	37.0	32.0	37.0
5	35.006150537830365	37.0	35.0	37.0	32.0	37.0
6	35.04452260436552	37.0	35.0	37.0	32.0	37.0
7	35.72378800235087	37.0	35.0	37.0	35.0	37.0
8	35.75457622794349	37.0	35.0	37.0	35.0	37.0
9	37.453843174953185	39.0	37.0	39.0	35.0	39.0
10	36.61311294665433	39.0	35.0	39.0	32.0	39.0
11	36.203980992560126	38.0	35.0	39.0	31.0	39.0
12	34.990867134715	35.0	34.0	39.0	30.0	39.0
13	34.41554035891805	35.0	33.0	39.0	26.0	39.0
14	35.27466388449746	36.0	33.0	40.0	27.0	41.0
15	35.785869253233585	36.0	34.0	40.0	30.0	41.0
16	36.05263584715686	36.0	34.0	40.0	31.0	41.0
17	36.02576209719672	36.0	34.0	40.0	31.0	41.0
18	35.95468283726588	36.0	34.0	40.0	30.0	41.0
19	35.84743932608329	36.0	34.0	40.0	30.0	41.0
20	35.677053937938794	36.0	34.0	40.0	30.0	41.0
21	35.477084918425646	35.0	34.0	40.0	30.0	41.0
22	35.37142414564474	35.0	34.0	40.0	29.0	41.0
23	35.357324379365174	35.0	34.0	40.0	29.0	41.0
24	35.296231770489264	35.0	34.0	40.0	29.0	41.0
25	35.235970167613544	35.0	34.0	40.0	29.0	41.0
26	35.071819146852064	35.0	34.0	40.0	29.0	41.0
27	34.96529547639332	35.0	34.0	40.0	29.0	41.0
28	35.0019873071123	36.0	34.0	40.0	29.0	41.0
29	35.056867417184144	36.0	34.0	40.0	29.0	41.0
30	34.99784230021003	36.0	34.0	40.0	29.0	41.0
31	34.77478552846788	35.0	34.0	40.0	28.0	41.0
32	34.56254003544532	35.0	33.0	40.0	27.0	41.0
33	34.316092084941204	35.0	33.0	40.0	25.0	41.0
34	34.15695443590456	35.0	33.0	40.0	25.0	41.0
35	34.02158428742602	35.0	33.0	40.0	24.0	41.0
36	33.73738388012374	35.0	33.0	39.0	23.0	41.0
37	33.68556445991444	35.0	33.0	39.0	23.0	41.0
38	33.743962677625255	35.0	33.0	39.0	23.0	41.0
39	33.732215605964655	35.0	33.0	39.0	23.0	41.0
40	33.5730879800267	35.0	33.0	39.0	23.0	41.0
41	33.576682627692	35.0	33.0	39.0	23.0	41.0
42	33.52902370462839	35.0	33.0	39.0	23.0	41.0
43	33.436773837890044	35.0	33.0	39.0	23.0	41.0
44	33.3372991393803	35.0	32.0	39.0	23.0	41.0
45	33.2805602000975	35.0	32.0	39.0	23.0	41.0
46	33.22439895577536	35.0	32.0	39.0	23.0	41.0
47	33.14665889117193	35.0	32.0	38.0	23.0	40.0
48	33.14304328611846	35.0	32.0	38.0	23.0	40.0
49	33.134504517228336	35.0	32.0	38.0	23.0	40.0
50	33.02397616324894	35.0	32.0	38.0	23.0	40.0
51	32.84249611604926	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	12.0
15	44.0
16	147.0
17	416.0
18	915.0
19	1740.0
20	2913.0
21	4721.0
22	6848.0
23	9664.0
24	13086.0
25	17583.0
26	22139.0
27	25250.0
28	27876.0
29	31761.0
30	37966.0
31	46033.0
32	56563.0
33	72245.0
34	121023.0
35	181960.0
36	72031.0
37	80108.0
38	106069.0
39	158198.0
40	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.677789268906068	24.74976422938317	31.011558455167137	13.560888046543626
2	29.91466698254614	26.553193040324746	30.807725075514934	12.724414901614175
3	26.714564929177698	26.2870342106582	33.46457518007408	13.533825680090025
4	24.352940640476007	28.70387666121471	32.54591262591518	14.397270072394107
5	23.0171349428	32.004665296843186	31.404008328283815	13.574191432073004
6	19.291093565626237	41.85892033003331	28.425963470361243	10.424022633979217
7	86.64841247784668	4.955966705088545	6.8975320397461415	1.4980887773186389
8	88.7312123849052	3.2625186224617644	6.199286537611678	1.8069824550213447
9	85.20034807488166	4.939838628111147	7.620106335965156	2.239706961042038
10	52.85179937401193	25.442086991384695	12.727148473983224	8.978965160620156
11	49.9738032647966	18.772808244454264	20.38935182443176	10.864036666317377
12	45.1864068557995	22.021659005070777	21.22035782462311	11.571576314506613
13	20.404659829698442	48.02804645250646	20.1425102395065	11.424783478288601
14	12.942827333901308	48.78123675925884	24.673224203049756	13.6027117037901
15	9.921136437152892	23.458606880401653	52.65115516212362	13.969101520321834
16	11.846026980359282	18.21716410090527	49.84997243647861	20.086836482256835
17	13.218735905017473	19.43005016105297	27.759062931391888	39.59215100253767
18	19.560168205819775	23.380153353409906	36.20206566952021	20.857612771250107
19	29.02744051063132	24.329705275339077	25.87754506977443	20.76530914425517
20	32.15883877845763	23.14752634480371	24.833867139270957	19.85976773746771
21	19.436975211054566	32.27446888966846	27.197769404946854	21.090786494330118
22	21.51731490298096	27.534545520813875	22.663319559165895	28.28482001703927
23	15.813625035878138	34.48820691320452	24.000856519342303	25.69731153157504
24	15.754944349022521	24.37417138587563	44.71805478990218	15.152829475199663
25	13.95598037295039	27.27613181285963	39.48882196698756	19.279065847202418
26	13.939487819657117	37.895787109383896	26.948012009494608	21.21671306146437
27	14.226695156565357	40.253128801374075	27.26993571548979	18.25024032657078
28	12.103347259365902	31.386422346042924	39.85666968878279	16.653560705808385
29	12.233920899527547	24.404514039172092	40.56184024091885	22.799724820381513
30	15.754853229943553	35.841416354963485	31.015020980167936	17.388709434925033
31	28.563826636840357	30.293813470133447	23.397283740255954	17.74507615277025
32	31.338584829584544	26.60622434428433	25.034875827475133	17.020314998655994
33	28.828618680322382	28.567198042762183	23.216047892187905	19.38813538472753
34	17.56657387707125	28.613577653957073	27.261188283908826	26.55866018506285
35	18.122309139699215	26.086936713243702	32.21187008241721	23.578884064639873
36	30.415366321477222	26.452779815301625	25.686559480256772	17.445294382964377
37	18.258714400914837	36.21628024583928	28.817502152688242	16.70750320055765
38	17.33923177504522	37.094577048015196	24.580373861581005	20.985817315358577
39	18.783651414851498	36.91325008086818	25.787519419753707	18.515579084526614
40	24.419093091807028	27.791774680741526	23.847867585754443	23.941264641697003
41	16.560619245260668	24.022633979215737	27.453814016847915	31.962932758675677
42	19.91380135129594	26.391001079761082	23.480384340275087	30.214813228667886
43	19.81839967561608	28.33220193810281	25.20745536304119	26.64194302323992
44	17.82936130081597	31.685292925059112	28.57284742565822	21.912498348466695
45	13.952973443344435	42.704960978254434	21.489523583895615	21.85254199450552
46	19.88810577102687	37.39399434150519	24.008419402896674	18.709480484571262
47	19.237697785350786	29.672107994332393	26.180607126423165	24.909587093893652
48	20.628812763960582	25.41393119598347	32.02844737645392	21.928808663602027
49	20.155904744114846	24.9408409379798	33.33764630307117	21.565608014834186
50	18.452069086485672	36.227852368868255	25.799273780940624	19.52080476370545
51	15.260987821935096	36.632512198566694	24.375993767454997	23.73050621204321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1059.0
1	1211.5
2	1364.0
3	5128.0
4	8892.0
5	6342.5
6	3793.0
7	4416.5
8	5040.0
9	5913.0
10	6786.0
11	7698.0
12	8610.0
13	8499.5
14	8389.0
15	7916.0
16	7443.0
17	6886.0
18	6329.0
19	6056.5
20	5784.0
21	5223.0
22	4662.0
23	5118.0
24	5574.0
25	5890.5
26	7433.0
27	8659.0
28	10163.0
29	11667.0
30	14101.5
31	16536.0
32	19069.5
33	21603.0
34	26113.5
35	30624.0
36	32650.5
37	34677.0
38	43269.0
39	51861.0
40	72434.0
41	93007.0
42	111257.0
43	129507.0
44	129770.5
45	130034.0
46	119104.5
47	108175.0
48	101028.5
49	93882.0
50	83315.0
51	72748.0
52	64996.0
53	57244.0
54	52075.5
55	46907.0
56	40492.0
57	34077.0
58	28764.5
59	23452.0
60	20416.5
61	17381.0
62	14823.0
63	12265.0
64	9905.0
65	7545.0
66	5971.5
67	4398.0
68	3742.5
69	3087.0
70	2795.5
71	2504.0
72	2199.5
73	1895.0
74	2022.5
75	1500.5
76	851.0
77	630.5
78	410.0
79	320.0
80	230.0
81	166.5
82	103.0
83	67.5
84	32.0
85	22.0
86	12.0
87	8.5
88	5.0
89	4.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1097465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.613138423580832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.61463791561398	15.61492913982204
2	8.493750841196581	3.331782219675893
3	3.1582036058595677	1.8582685347472747
4	1.5637725143606351	1.2268194714858471
5	0.887641369666092	0.8704716526878971
6	0.5853811893762452	0.6888697378678009
7	0.4259955918844446	0.5848577358025402
8	0.3380066213300623	0.5303496521786705
9	0.2540546902073323	0.44845288255767185
>10	2.60220364548842	11.521550510035034
>50	0.7682595690953866	10.77141224463507
>100	1.296438022846438	50.261500764402875
>500	0.010722069228863025	1.3930568124327012
>1k	4.6617692299404453E-4	0.31681162083744485
>5k	4.6617692299404453E-4	0.5808670208312516
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6353	0.5788795086859262	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3465	0.315727608625332	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03735882237702341	0.0
2	0.0	0.0	0.0	0.1493441704291253	0.0
3	0.0	0.0	0.0	0.2203259329454698	0.0
4	0.0	0.0	0.0	0.3372317112618626	0.0
5	0.0	0.0	0.0	0.6046662080339692	0.0
6	9.111907896834978E-5	0.0	0.0	0.913559885736675	0.0
7	9.111907896834978E-5	0.0	0.0	1.119853480521019	0.0
8	9.111907896834978E-5	0.0	0.0	1.6503487582747514	0.0
9	9.111907896834978E-5	0.0	0.0	1.9130450629405038	0.0
10	9.111907896834978E-5	0.0	0.0	2.3354731130377733	0.0
11	9.111907896834978E-5	0.0	0.0	2.6303344525793535	0.0
12	9.111907896834978E-5	0.0	0.0	2.9132591927760796	0.0
13	9.111907896834978E-5	0.0	0.0	3.019686277011112	0.0
14	9.111907896834978E-5	0.0	0.0	3.057956290177819	0.0
15	9.111907896834978E-5	0.0	0.0	3.108982974400095	0.0
16	9.111907896834978E-5	0.0	0.0	3.242381306009759	0.0
17	9.111907896834978E-5	0.0	0.0	3.3868050461745933	0.0
18	9.111907896834978E-5	0.0	0.0	3.6303663442569922	0.0
19	9.111907896834978E-5	0.0	0.0	3.7148337304606525	0.0
20	9.111907896834978E-5	0.0	0.0	3.8124222640357552	0.0
21	9.111907896834978E-5	0.0	0.0	3.926047755509287	0.0
22	9.111907896834978E-5	0.0	0.0	4.033841625928845	0.0
23	9.111907896834978E-5	0.0	0.0	4.158401406878579	0.0
24	9.111907896834978E-5	0.0	0.0	4.247606985188594	0.0
25	9.111907896834978E-5	0.0	0.0	4.323782535206134	0.0
26	1.8223815793669957E-4	0.0	0.0	4.396586679301846	0.0
27	1.8223815793669957E-4	0.0	0.0	4.473035586556291	0.0
28	1.8223815793669957E-4	0.0	0.0	4.554587162232965	0.0
29	1.8223815793669957E-4	0.0	0.0	4.646435193833061	0.0
30	1.8223815793669957E-4	0.0	0.0	4.767076854387156	0.0
31	1.8223815793669957E-4	0.0	0.0	4.864300911646385	0.0
32	1.8223815793669957E-4	0.0	0.0	4.954691037982988	0.0
33	1.8223815793669957E-4	0.0	0.0	5.046174593267211	0.0
34	1.8223815793669957E-4	0.0	0.0	5.1407561972363585	0.0
35	1.8223815793669957E-4	0.0	0.0	5.280077268978965	0.0
36	1.8223815793669957E-4	0.0	0.0	5.385046447950504	0.0
37	1.8223815793669957E-4	0.0	0.0	5.481268195341081	0.0
38	1.8223815793669957E-4	0.0	0.0	5.58350380194357	0.0
39	2.7335723690504935E-4	0.0	0.0	5.691479910521064	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	20	7.033146E-4	45.000004	12
CGTGCCG	40	6.8139343E-9	45.000004	41
TAGCGCT	20	7.033146E-4	45.000004	30
TAGCGCG	40	6.8139343E-9	45.000004	1
ACGATTC	20	7.033146E-4	45.000004	35
CTAACGG	40	6.8139343E-9	45.000004	2
CCTCCGT	20	7.033146E-4	45.000004	38
TCTACGA	40	6.8139343E-9	45.000004	13
CCATCCG	20	7.033146E-4	45.000004	30
CTACGTC	20	7.033146E-4	45.000004	32
CGGATCT	20	7.033146E-4	45.000004	15
GGGCGTA	40	6.8139343E-9	45.000004	7
GTATGAC	40	6.8139343E-9	45.000004	16
TACGACT	20	7.033146E-4	45.000004	40
TATTCGA	20	7.033146E-4	45.000004	13
TCGCTGA	20	7.033146E-4	45.000004	36
TCGCTAA	40	6.8139343E-9	45.000004	43
AGCGCGA	20	7.033146E-4	45.000004	31
AGCGATC	20	7.033146E-4	45.000004	39
AACGACC	20	7.033146E-4	45.000004	31
>>END_MODULE