FastQCFastQC Report
Thu 26 May 2016
SRR1545191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545191_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2400469
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171980.7164433283662485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA207.034824E-445.012
CGTTTTT109600.042.967611
TCACGAC5500.040.90909225
ACGGGTA2050.040.6097535
ACATACG7500.040.19999717
CGGTCTA5700.039.47368631
TCGACGG750.039.02
GGGTACC12350.038.987857
GTAGGGC12400.038.8306434
CATACGA7800.038.65384718
CGTACGT356.2511335E-638.57142621
ACGTCGT356.2511335E-638.57142645
CGTTAGG2700.038.3333322
AAGGGAT30800.038.1331185
AGGGATG44500.037.8202256
AGGGTAT13700.037.773726
TATGGGA22650.037.7483444
GACCGAT9900.037.7272729
GTTGATC11700.037.69230716
TTAGGGA24900.037.5903634
Produced by FastQC (version 0.11.5)