##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545191_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2400469
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98948913733108	31.0	31.0	33.0	28.0	34.0
2	31.281094236168016	31.0	31.0	34.0	28.0	34.0
3	31.384382801860802	31.0	31.0	34.0	28.0	34.0
4	35.18002856941706	37.0	35.0	37.0	32.0	37.0
5	35.035353924587234	37.0	35.0	37.0	32.0	37.0
6	35.09071727233303	37.0	35.0	37.0	32.0	37.0
7	35.70488558694155	37.0	35.0	37.0	35.0	37.0
8	35.69640974326267	37.0	35.0	37.0	35.0	37.0
9	37.37910716614128	39.0	37.0	39.0	35.0	39.0
10	36.54103094020377	39.0	35.0	39.0	32.0	39.0
11	36.252023250456475	38.0	35.0	39.0	31.0	39.0
12	35.76945005330209	37.0	35.0	39.0	30.0	39.0
13	35.5798050297671	37.0	35.0	39.0	30.0	39.0
14	36.517607184262744	38.0	35.0	40.0	30.0	41.0
15	36.72192975622681	38.0	35.0	40.0	31.0	41.0
16	36.7782645807965	38.0	35.0	41.0	31.0	41.0
17	36.69147112501765	38.0	35.0	40.0	31.0	41.0
18	36.58716609129299	38.0	35.0	40.0	31.0	41.0
19	36.513810426212544	38.0	35.0	40.0	30.0	41.0
20	36.38724099332256	38.0	35.0	40.0	30.0	41.0
21	36.29123100527438	38.0	35.0	40.0	30.0	41.0
22	36.20146063123498	38.0	34.0	40.0	30.0	41.0
23	36.12959967406369	38.0	34.0	40.0	30.0	41.0
24	36.055437499921894	38.0	34.0	40.0	30.0	41.0
25	35.935480941432694	38.0	34.0	40.0	30.0	41.0
26	35.80104762860924	38.0	34.0	40.0	29.0	41.0
27	35.68070156290292	38.0	34.0	40.0	29.0	41.0
28	35.65796225654237	38.0	34.0	40.0	29.0	41.0
29	35.602822198495375	37.0	34.0	40.0	29.0	41.0
30	35.484122894317736	37.0	34.0	40.0	28.0	41.0
31	35.34651353547994	37.0	34.0	40.0	27.0	41.0
32	35.193387208916256	37.0	34.0	40.0	27.0	41.0
33	34.97959648718646	37.0	33.0	40.0	25.0	41.0
34	34.74895197563476	37.0	33.0	40.0	25.0	41.0
35	34.56862971361013	37.0	33.0	40.0	24.0	41.0
36	34.43136112151417	37.0	33.0	40.0	23.0	41.0
37	34.385037257302635	37.0	33.0	40.0	23.0	41.0
38	34.30700375634928	37.0	33.0	40.0	23.0	41.0
39	34.23439419546764	37.0	33.0	40.0	23.0	41.0
40	34.07995729167925	37.0	33.0	40.0	23.0	41.0
41	33.98085832393586	36.0	33.0	40.0	23.0	41.0
42	33.90611626311358	36.0	33.0	40.0	23.0	41.0
43	33.85068417880006	36.0	33.0	40.0	23.0	41.0
44	33.730376022352296	36.0	32.0	40.0	23.0	41.0
45	33.661043737702926	36.0	32.0	40.0	23.0	41.0
46	33.59292121664558	35.0	32.0	40.0	23.0	41.0
47	33.482648182501	35.0	32.0	40.0	22.0	41.0
48	33.3993169668094	35.0	32.0	39.0	22.0	41.0
49	33.351120968444086	35.0	32.0	39.0	22.0	41.0
50	33.30851554425406	35.0	32.0	39.0	22.0	41.0
51	33.14538825537843	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	6.0
13	6.0
14	22.0
15	104.0
16	361.0
17	841.0
18	1873.0
19	3637.0
20	5966.0
21	9200.0
22	13558.0
23	19051.0
24	26286.0
25	36117.0
26	46638.0
27	53837.0
28	58983.0
29	66964.0
30	78352.0
31	92561.0
32	112065.0
33	139102.0
34	200088.0
35	249483.0
36	202633.0
37	241858.0
38	317891.0
39	422798.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.11670802663979	22.671819548596545	28.272308453056468	14.939163971707195
2	32.60187904946908	25.02885894381473	29.082650098793195	13.286611907922993
3	28.997958315645818	24.69325785919335	32.72006428743716	13.58871953772367
4	26.367722307599056	27.484545728355585	31.2162331611031	14.931498802942258
5	22.75521991744113	31.27438846325447	31.18719716855331	14.783194450751083
6	19.871825047521963	41.384329478947656	28.466645476363162	10.277199997167221
7	86.66981327398938	3.763056302747505	7.866629396172165	1.700501027090956
8	87.40729415793331	3.1010606677278485	7.717033629678201	1.7746115446606476
9	82.84243620725783	5.044597534898389	9.1267581460123	2.9862081118314796
10	40.22205660643816	30.62030794815513	15.897143433220759	13.260492012185951
11	34.079465304488416	24.17365106568758	25.441070057559585	16.30581357226442
12	32.239324898592734	21.634938839035208	28.628905434729628	17.49683082764243
13	24.57578081616551	28.191740863972832	28.892145659868966	18.340332659992693
14	19.92827235011158	30.303161590505855	30.7594474246491	19.00911863473346
15	18.57257894186511	23.617259793815293	38.19028698141905	19.619874282900547
16	21.788992067800084	21.116790093935812	35.7368497572766	21.357368080987506
17	21.623482744413696	21.868684827839893	30.216386881063663	26.291445546682752
18	23.377098392022557	23.142644208277634	32.63433104114238	20.84592635855743
19	27.099079388236213	25.166207103695154	26.52048412206115	21.214229386007485
20	28.493515225566334	24.98595066214144	26.817176143495292	19.70335796879693
21	24.868182009432324	26.414088246921747	28.963215105048224	19.754514638597705
22	24.26571640791862	23.43087954895481	28.2638101137736	24.03959392935297
23	21.783701435011242	26.939735526682497	28.116213956522664	23.1603490817836
24	21.507088823059163	24.284837671305066	33.772233676002486	20.43583982963329
25	22.04035961305895	25.046063915009942	31.070011735206744	21.843564736724367
26	20.72011761035031	27.793943600188133	27.835977052817597	23.649961736643967
27	20.100363720589602	27.160942299192364	30.09053647433064	22.648157505887394
28	19.68486158329893	26.185882842061282	32.534767164249985	21.594488410389804
29	20.830304411346283	24.99557378162351	31.767667068393717	22.40645473863649
30	22.537679095210144	27.036175014132656	30.619558094688998	19.806587795968206
31	26.36597264951141	25.407784895368362	27.3617780525389	20.86446440258133
32	26.909199827200435	25.346838471981936	26.6737041803081	21.070257520509532
33	26.930112407200426	25.615827573694972	26.736983481144726	20.717076537959876
34	21.826693033736326	26.504778857798208	28.875357273932718	22.793170834532752
35	21.740376568078986	26.26986643026842	29.830503955685327	22.15925304596727
36	26.25724389692181	27.600689698554742	25.574294023376265	20.567772381147183
37	22.04035961305895	30.427345656203013	26.432876242101024	21.099418488637014
38	22.826705947879354	29.634292298713294	26.070155457121086	21.468846296286266
39	22.733265874293732	28.611825439112103	25.829202543336322	22.825706143257836
40	24.57715554752009	26.55664372253922	26.5337315332962	22.33246919664449
41	20.819889779872184	25.15695891094615	28.261560553375194	25.76159075580647
42	21.994951819831872	26.803803756682548	25.541967007280657	25.65927741620492
43	21.53916588799939	26.35776591990982	27.04575647508883	25.05731171700197
44	22.338634658477154	26.35201704333611	28.114714249590396	23.19463404859634
45	20.941865943696836	29.601965282617687	26.476742669869928	22.979426103815545
46	22.218366494214255	29.07506824708005	26.908824900467366	21.797740358238325
47	20.852924990908026	28.1771187213832	27.889799868275738	23.080156419433035
48	21.722338426365848	26.414879758913777	29.351139298195477	22.511642516524898
49	22.078477164254153	25.795542454412036	29.609547134330832	22.516433247002983
50	21.531917304493415	27.775405556164234	28.09984215584538	22.592834983496974
51	20.785938081266618	28.361957600785516	27.581276825487016	23.27082749246085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1136.0
1	2216.0
2	3296.0
3	16264.5
4	29233.0
5	20823.5
6	12414.0
7	12553.0
8	12692.0
9	13114.0
10	13536.0
11	13698.5
12	13861.0
13	13353.0
14	12845.0
15	12265.0
16	11685.0
17	11387.0
18	11089.0
19	10479.5
20	9870.0
21	9963.0
22	10056.0
23	10823.0
24	11590.0
25	13093.5
26	17641.5
27	20686.0
28	23646.0
29	26606.0
30	32290.5
31	37975.0
32	43907.5
33	49840.0
34	59861.5
35	69883.0
36	76636.0
37	83389.0
38	90289.0
39	97189.0
40	112874.5
41	128560.0
42	142704.0
43	156848.0
44	161831.5
45	166815.0
46	169329.5
47	171844.0
48	168383.0
49	164922.0
50	161860.0
51	158798.0
52	153596.0
53	148394.0
54	141054.5
55	133715.0
56	128334.0
57	122953.0
58	117995.0
59	113037.0
60	108376.0
61	103715.0
62	93589.5
63	83464.0
64	72160.0
65	60856.0
66	52791.0
67	44726.0
68	37569.5
69	30413.0
70	25882.0
71	21351.0
72	18882.5
73	16414.0
74	13041.0
75	7634.5
76	5601.0
77	4044.5
78	2488.0
79	1797.5
80	1107.0
81	961.5
82	816.0
83	563.5
84	311.0
85	208.0
86	105.0
87	76.5
88	48.0
89	31.0
90	14.0
91	14.5
92	15.0
93	9.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2400469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.022489511846768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41016040478395	15.499441248151626
2	6.829954667298263	2.7350539138473677
3	2.886314876782646	1.733736280448035
4	1.6990090686243093	1.3607356502825114
5	1.131871521785968	1.1331442836858794
6	0.8683811498318486	1.0432291478876174
7	0.6891626252844107	0.9659126005699727
8	0.5421610594328734	0.8684331300981153
9	0.47838588398081566	0.8620628710159286
>10	5.268549343757707	24.16007205094271
>50	1.2568380817316056	17.849319711456886
>100	0.9335685277062732	30.111568406065608
>500	0.0037618593328683243	0.5015175786740145
>1k	0.0016719374812748109	0.4561152780598045
>5k	0.0	0.0
>10k+	2.0899218515935136E-4	0.7196578488139924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17198	0.7164433283662485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1658525896397746E-5	0.0	0.0	0.016371800677284314	0.0
2	4.1658525896397746E-5	0.0	0.0	0.054905937131452226	0.0
3	4.1658525896397746E-5	0.0	0.0	0.08552495366530458	0.0
4	4.1658525896397746E-5	0.0	0.0	0.1219345052987562	0.0
5	4.1658525896397746E-5	0.0	0.0	0.20637633729075444	0.0
6	4.1658525896397746E-5	0.0	0.0	0.34630732577675444	0.0
7	4.1658525896397746E-5	0.0	0.0	0.44324671553767203	0.0
8	4.1658525896397746E-5	0.0	0.0	0.7016545516730273	0.0
9	4.1658525896397746E-5	0.0	0.0	0.8460846609558382	0.0
10	4.1658525896397746E-5	0.0	0.0	1.066749872629057	0.0
11	4.1658525896397746E-5	0.0	0.0	1.2056810564935436	0.0
12	4.1658525896397746E-5	0.0	0.0	1.34357077721062	0.0
13	8.331705179279549E-5	0.0	0.0	1.4063501757364916	0.0
14	8.331705179279549E-5	0.0	0.0	1.4365526070113799	0.0
15	8.331705179279549E-5	0.0	0.0	1.4662551359755114	0.0
16	8.331705179279549E-5	0.0	0.0	1.5229107311946124	0.0
17	8.331705179279549E-5	0.0	0.0	1.586023397927655	0.0
18	8.331705179279549E-5	0.0	0.0	1.6915027854973341	0.0
19	8.331705179279549E-5	0.0	0.0	1.736743944620822	0.0
20	8.331705179279549E-5	0.0	0.0	1.7923997352184093	0.0
21	8.331705179279549E-5	0.0	0.0	1.851429866413605	0.0
22	8.331705179279549E-5	0.0	0.0	1.9156673133458504	0.0
23	8.331705179279549E-5	0.0	0.0	1.9880281728278932	0.0
24	8.331705179279549E-5	0.0	0.0	2.0470166454971923	0.0
25	8.331705179279549E-5	0.0	0.0	2.0973818033059373	0.0
26	8.331705179279549E-5	0.0	0.0	2.1481218878477497	0.0
27	8.331705179279549E-5	0.0	0.0	2.210359725536968	0.0
28	1.2497557768919324E-4	0.0	0.0	2.2645574677281815	0.0
29	1.2497557768919324E-4	0.0	0.0	2.3345021327082334	0.0
30	1.6663410358559098E-4	0.0	0.0	2.4354823994811015	0.0
31	1.6663410358559098E-4	0.0	0.0	2.512842282070712	0.0
32	1.6663410358559098E-4	0.0	0.0	2.587619336054746	0.0
33	1.6663410358559098E-4	0.0	0.0	2.663312877608501	0.0
34	1.6663410358559098E-4	0.0	0.0	2.7451718809949224	0.0
35	1.6663410358559098E-4	0.0	0.0	2.8481100984849212	0.0
36	2.0829262948198873E-4	0.0	0.0	2.9309689064928564	0.0
37	2.0829262948198873E-4	0.0	0.0	3.0147025435446158	0.0
38	2.0829262948198873E-4	0.0	0.0	3.130138318803534	0.0
39	2.0829262948198873E-4	0.0	0.0	3.321934172030549	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGA	20	7.034824E-4	45.0	12
CGTTTTT	10960	0.0	42.96761	1
TCACGAC	550	0.0	40.909092	25
ACGGGTA	205	0.0	40.609753	5
ACATACG	750	0.0	40.199997	17
CGGTCTA	570	0.0	39.473686	31
TCGACGG	75	0.0	39.0	2
GGGTACC	1235	0.0	38.98785	7
GTAGGGC	1240	0.0	38.830643	4
CATACGA	780	0.0	38.653847	18
CGTACGT	35	6.2511335E-6	38.571426	21
ACGTCGT	35	6.2511335E-6	38.571426	45
CGTTAGG	270	0.0	38.333332	2
AAGGGAT	3080	0.0	38.133118	5
AGGGATG	4450	0.0	37.820225	6
AGGGTAT	1370	0.0	37.77372	6
TATGGGA	2265	0.0	37.748344	4
GACCGAT	990	0.0	37.727272	9
GTTGATC	1170	0.0	37.692307	16
TTAGGGA	2490	0.0	37.590363	4
>>END_MODULE