FastQCFastQC Report
Thu 26 May 2016
SRR1545190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545190_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1730441
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60970.35233793004211067No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36950.21352938355020484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT302.16614E-645.00000443
CTATACG207.034277E-445.01
ACTTACG351.212411E-745.01
TACGAAC1000.045.034
CATACGT207.034277E-445.023
CGTAAGA207.034277E-445.021
CAATCGT351.212411E-745.017
TACGTAA207.034277E-445.032
TATCCGT207.034277E-445.016
TATCCGC207.034277E-445.029
CGGGTAT1700.043.6764686
CGACGGT5500.042.54545228
CGTTTTT51100.042.1819951
CACGCAT750.042.032
ATGACGT700.041.78571340
ACGTAGG3300.041.5909082
TCTCACG5750.041.08695623
CGTTAGG1550.040.645162
CTCACGA5900.040.4237324
TCGTACA451.9292202E-840.034