##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545190_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1730441
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07105876478886	31.0	31.0	33.0	30.0	34.0
2	31.351287908689173	31.0	31.0	34.0	30.0	34.0
3	31.469338162930722	31.0	31.0	34.0	30.0	34.0
4	35.24963405282237	37.0	35.0	37.0	33.0	37.0
5	35.10151458501041	37.0	35.0	37.0	32.0	37.0
6	35.15156541020468	37.0	35.0	37.0	32.0	37.0
7	35.754250506084865	37.0	35.0	37.0	35.0	37.0
8	35.751363958667184	37.0	35.0	37.0	35.0	37.0
9	37.44815454557538	39.0	37.0	39.0	35.0	39.0
10	36.635940202526406	39.0	35.0	39.0	32.0	39.0
11	36.328516256838576	38.0	35.0	39.0	32.0	39.0
12	35.582505846775476	37.0	35.0	39.0	30.0	39.0
13	35.248703654155214	37.0	35.0	39.0	30.0	39.0
14	36.13155085900068	38.0	35.0	40.0	30.0	41.0
15	36.45960769537938	38.0	35.0	40.0	30.0	41.0
16	36.60096472517699	38.0	35.0	40.0	31.0	41.0
17	36.55437544533446	38.0	35.0	40.0	31.0	41.0
18	36.50807279762789	38.0	35.0	40.0	31.0	41.0
19	36.444324308081	38.0	35.0	40.0	31.0	41.0
20	36.31114091725751	38.0	35.0	40.0	30.0	41.0
21	36.17533449565747	38.0	34.0	40.0	30.0	41.0
22	36.08958352235066	38.0	34.0	40.0	30.0	41.0
23	36.03438834378058	38.0	34.0	40.0	30.0	41.0
24	35.98223343066883	37.0	34.0	40.0	30.0	41.0
25	35.872129705664626	37.0	34.0	40.0	30.0	41.0
26	35.73498200747671	37.0	34.0	40.0	30.0	41.0
27	35.61779800640415	37.0	34.0	40.0	29.0	41.0
28	35.633402699080754	37.0	34.0	40.0	29.0	41.0
29	35.603356023117804	37.0	34.0	40.0	29.0	41.0
30	35.54009758206145	37.0	34.0	40.0	29.0	41.0
31	35.367567573815	37.0	34.0	40.0	29.0	41.0
32	35.263640309031054	37.0	34.0	40.0	28.0	41.0
33	35.07354194682165	37.0	34.0	40.0	27.0	41.0
34	34.90156902200075	37.0	34.0	40.0	26.0	41.0
35	34.759473452143126	37.0	33.0	40.0	26.0	41.0
36	34.569921193499226	36.0	33.0	40.0	24.0	41.0
37	34.50471816143977	36.0	33.0	40.0	24.0	41.0
38	34.43760925683106	36.0	33.0	40.0	24.0	41.0
39	34.37640867270251	36.0	33.0	40.0	24.0	41.0
40	34.21906438878875	36.0	33.0	40.0	23.0	41.0
41	34.158292597089414	36.0	33.0	40.0	23.0	41.0
42	34.09460247416699	36.0	33.0	40.0	23.0	41.0
43	34.049544017969986	35.0	33.0	40.0	23.0	41.0
44	33.91995508659353	35.0	33.0	40.0	23.0	41.0
45	33.861065474061235	35.0	33.0	40.0	23.0	41.0
46	33.815785109113804	35.0	33.0	39.0	23.0	41.0
47	33.72418302617656	35.0	33.0	39.0	23.0	41.0
48	33.637984768044674	35.0	32.0	39.0	23.0	41.0
49	33.59107533859866	35.0	32.0	39.0	24.0	41.0
50	33.5196669519504	35.0	32.0	39.0	23.0	41.0
51	33.32930854042409	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	22.0
15	50.0
16	192.0
17	527.0
18	1062.0
19	2044.0
20	3694.0
21	5806.0
22	8605.0
23	12205.0
24	17135.0
25	23203.0
26	29955.0
27	35600.0
28	40738.0
29	46883.0
30	55397.0
31	67361.0
32	83015.0
33	105101.0
34	160666.0
35	213954.0
36	137275.0
37	167127.0
38	217686.0
39	294935.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.25250615305578	24.32628445581213	29.326859453746184	13.094349937385905
2	30.269567121907077	26.12218503838039	30.811683264555107	12.796564575157433
3	28.77439912715892	24.954563605462425	32.70310862953432	13.567928637844343
4	26.501683674855137	27.710392899844607	30.98608967309489	14.801833752205361
5	23.12780383728772	33.052325967773534	29.668679833637785	14.151190361300964
6	20.355504752834683	41.99865814552475	27.272180906485687	10.373656195154876
7	88.39943112767207	4.044229187819752	5.999164374861668	1.5571753096465004
8	89.50897487981388	3.225593938192634	5.706002111600453	1.5594290703930385
9	84.99272728743713	5.210810423470087	7.076173068021389	2.7202892210713916
10	45.26666901674197	30.302159969626242	12.743225570822696	11.687945442809088
11	41.3296957249626	22.569102327094654	21.561266752232523	14.539935195710227
12	37.52251593668897	21.819813561976396	24.4871105111356	16.17055999019903
13	24.110443522778297	34.290449659942176	25.255353982019614	16.343752835259913
14	18.86339956115233	35.60208062569021	28.673211048513064	16.861308764644388
15	16.363054273448213	23.56451332348228	41.87851536111315	18.193917041956357
16	19.866438670835933	19.620489805777833	39.11222630531755	21.40084521806869
17	19.942084127687682	20.36608009172228	28.094976945183337	31.5968588354067
18	23.516375305485713	22.62041872563121	32.46929539926528	21.393910569617802
19	28.90199665865522	24.030232755696378	24.971958015326727	22.09581257032167
20	29.50334625682124	24.319985483469242	24.93173705431159	21.24493120539793
21	24.40320126487988	27.162093362327873	28.65385182158768	19.780853551204576
22	24.5481354175034	23.16080120616652	25.74268640190564	26.548376974424436
23	20.34591182247762	28.782027240454887	26.403038300641278	24.469022636426203
24	20.725526036426555	25.5742322332862	34.473408801571395	19.226832928715858
25	20.397286009751273	25.62086774411841	32.46507682145765	21.51676942467267
26	19.74236625230216	30.43987053011342	25.94067061517844	23.877092602405977
27	19.57269852020381	31.266538414196148	27.895490224746176	21.265272840853864
28	18.387798254895717	27.62313190683762	33.797569521295436	20.191500316971222
29	18.868253815067952	25.203286329900877	33.36120676752342	22.56725308750775
30	22.54303960666674	28.66870352701999	29.179093653005218	19.609163213308054
31	27.646767500307725	26.73220294711001	25.21490186605611	20.40612768652615
32	28.595485197126052	25.049857232925017	26.271684501234077	20.082973068714853
33	27.610533962151845	26.05977320232241	24.940231998663926	21.38946083686182
34	21.174775678569798	26.88459184681824	28.09081615611281	23.849816318499155
35	21.97370496884898	25.81035701303887	28.652811624320044	23.563126393792103
36	29.620772970589577	25.74031706368492	24.558710756391	20.0801992093345
37	21.69764817176662	31.069767764402258	26.960295092407083	20.272288971424047
38	21.800858856210642	30.89120056679193	24.36488733218873	22.94305324480869
39	21.74671080955664	29.424291264481134	25.722864865083523	23.1061330608787
40	24.71208206462977	24.9130135034942	26.045210440575552	24.329693991300484
41	20.899585712543796	23.011648475735377	27.802565935504305	28.28619987621653
42	21.756188162439514	25.45380050518914	25.492461170302832	27.29755016206851
43	21.46574196982157	25.682586115331297	27.162035573590774	25.68963634125636
44	21.372182004471693	27.2939094716318	28.68817833141956	22.645730192476947
45	19.28462166580658	32.85122116269783	24.404125884673327	23.46003128682226
46	22.230518116480134	30.061989978277214	26.5548493129786	21.152642592264055
47	21.99190842103256	27.292695908152893	27.245598087423957	23.46979758339059
48	22.00832042236632	27.132274373989056	28.97261449538008	21.88679070826454
49	22.601464019865457	25.471137126316357	29.710287724343097	22.217111129475086
50	22.239244215780833	29.67931296126248	26.61038428932278	21.47105853363391
51	20.057488235657846	30.83861281603938	25.6974956095007	23.406403338802072
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1010.0
1	1620.5
2	2231.0
3	6535.5
4	10840.0
5	8189.0
6	5538.0
7	5804.0
8	6070.0
9	6646.0
10	7222.0
11	7446.0
12	7670.0
13	7549.0
14	7428.0
15	6964.5
16	6501.0
17	6397.0
18	6293.0
19	6236.0
20	6179.0
21	6112.0
22	6045.0
23	6718.5
24	7392.0
25	7789.0
26	10895.0
27	13604.0
28	16785.0
29	19966.0
30	22277.5
31	24589.0
32	29642.0
33	34695.0
34	40024.0
35	45353.0
36	47878.5
37	50404.0
38	59226.5
39	68049.0
40	88603.5
41	109158.0
42	124739.5
43	140321.0
44	147041.5
45	153762.0
46	143766.0
47	133770.0
48	130871.5
49	127973.0
50	123250.0
51	118527.0
52	111293.5
53	104060.0
54	101095.0
55	98130.0
56	93329.0
57	88528.0
58	83230.0
59	77932.0
60	69408.0
61	60884.0
62	54315.5
63	47747.0
64	43871.0
65	39995.0
66	34400.0
67	28805.0
68	23910.5
69	19016.0
70	16643.0
71	14270.0
72	12053.0
73	9836.0
74	7949.0
75	4639.0
76	3216.0
77	2463.0
78	1710.0
79	1296.5
80	883.0
81	614.0
82	345.0
83	255.0
84	165.0
85	104.0
86	43.0
87	32.5
88	22.0
89	17.0
90	12.0
91	7.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1730441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.790158035453526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.53428968596654	14.14927582750886
2	7.041406941526139	2.5053548456337897
3	2.701031305231517	1.44155321336328
4	1.4086779810057546	1.002424156126239
5	0.8774737470728757	0.7805198316194019
6	0.6268877046238395	0.6691458801444489
7	0.4912727218618942	0.6117873552301352
8	0.3766357218747093	0.536032721115856
9	0.3542630934346886	0.5672156776498575
>10	3.9125104066440195	16.330330430508226
>50	1.2459466338671439	15.958007929868897
>100	1.417576919531735	43.45564707372512
>500	0.010076790760256379	1.149395099189967
>1k	0.001625288832299416	0.4907308719926576
>5k	3.250577664598832E-4	0.35257908632326573
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6097	0.35233793004211067	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3695	0.21352938355020484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.7788737090718495E-5	0.0	0.0	0.02565819926827901	0.0
2	5.7788737090718495E-5	0.0	0.0	0.09182630323715169	0.0
3	5.7788737090718495E-5	0.0	0.0	0.1287533062381208	0.0
4	5.7788737090718495E-5	0.0	0.0	0.18209231057285397	0.0
5	1.1557747418143699E-4	0.0	0.0	0.29645622127538585	0.0
6	1.1557747418143699E-4	0.0	0.0	0.4190839213818905	0.0
7	1.1557747418143699E-4	0.0	0.0	0.5015484492103458	0.0
8	1.1557747418143699E-4	0.0	0.0	0.7038090290278606	0.0
9	1.1557747418143699E-4	0.0	0.0	0.7988137128050017	0.0
10	1.1557747418143699E-4	0.0	0.0	0.966458839105176	0.0
11	1.1557747418143699E-4	0.0	0.0	1.1108728930948817	0.0
12	1.7336621127215547E-4	0.0	0.0	1.2434980447180806	0.0
13	1.7336621127215547E-4	0.0	0.0	1.2989174435880795	0.0
14	1.7336621127215547E-4	0.0	0.0	1.320183698837464	0.0
15	1.7336621127215547E-4	0.0	0.0	1.351794138026087	0.0
16	1.7336621127215547E-4	0.0	0.0	1.420967256323677	0.0
17	1.7336621127215547E-4	0.0	0.0	1.5059166998470332	0.0
18	1.7336621127215547E-4	0.0	0.0	1.6458232323436628	0.0
19	1.7336621127215547E-4	0.0	0.0	1.700549166368573	0.0
20	1.7336621127215547E-4	0.0	0.0	1.7659082280181757	0.0
21	1.7336621127215547E-4	0.0	0.0	1.839588867808842	0.0
22	1.7336621127215547E-4	0.0	0.0	1.9140207611816873	0.0
23	1.7336621127215547E-4	0.0	0.0	2.0061360081042925	0.0
24	1.7336621127215547E-4	0.0	0.0	2.073748830500433	0.0
25	1.7336621127215547E-4	0.0	0.0	2.1288792856849788	0.0
26	1.7336621127215547E-4	0.0	0.0	2.188979572259326	0.0
27	1.7336621127215547E-4	0.0	0.0	2.25930846529873	0.0
28	1.7336621127215547E-4	0.0	0.0	2.32281828736143	0.0
29	1.7336621127215547E-4	0.0	0.0	2.403664730551345	0.0
30	1.7336621127215547E-4	0.0	0.0	2.502830203399018	0.0
31	1.7336621127215547E-4	0.0	0.0	2.5856414636500173	0.0
32	1.7336621127215547E-4	0.0	0.0	2.656201511637785	0.0
33	1.7336621127215547E-4	0.0	0.0	2.7390127718887842	0.0
34	1.7336621127215547E-4	0.0	0.0	2.825349145102318	0.0
35	1.7336621127215547E-4	0.0	0.0	2.9296578155510646	0.0
36	1.7336621127215547E-4	0.0	0.0	3.0131047519100624	0.0
37	1.7336621127215547E-4	0.0	0.0	3.105335576306849	0.0
38	1.7336621127215547E-4	0.0	0.0	3.2132271484552204	0.0
39	1.7336621127215547E-4	0.0	0.0	3.3857843174081057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGAT	30	2.16614E-6	45.000004	43
CTATACG	20	7.034277E-4	45.0	1
ACTTACG	35	1.212411E-7	45.0	1
TACGAAC	100	0.0	45.0	34
CATACGT	20	7.034277E-4	45.0	23
CGTAAGA	20	7.034277E-4	45.0	21
CAATCGT	35	1.212411E-7	45.0	17
TACGTAA	20	7.034277E-4	45.0	32
TATCCGT	20	7.034277E-4	45.0	16
TATCCGC	20	7.034277E-4	45.0	29
CGGGTAT	170	0.0	43.676468	6
CGACGGT	550	0.0	42.545452	28
CGTTTTT	5110	0.0	42.181995	1
CACGCAT	75	0.0	42.0	32
ATGACGT	70	0.0	41.785713	40
ACGTAGG	330	0.0	41.590908	2
TCTCACG	575	0.0	41.086956	23
CGTTAGG	155	0.0	40.64516	2
CTCACGA	590	0.0	40.42373	24
TCGTACA	45	1.9292202E-8	40.0	34
>>END_MODULE