FastQCFastQC Report
Thu 26 May 2016
SRR1545189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545189_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1487756
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65160.43797504429489775No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33700.22651563831703586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT302.1659343E-645.00000424
CCAATCG302.1659343E-645.00000424
GCGATAC453.8562575E-1045.0000049
TCGTAAG1200.045.0000041
ACGTAAG302.1659343E-645.0000041
TCACGCA207.033957E-445.019
TCGTTAC253.891362E-545.01
CGAACTA207.033957E-445.020
CGAACAC253.891362E-545.042
CGAAACG207.033957E-445.020
ACGTTAC207.033957E-445.019
GACACGT207.033957E-445.012
GACCGTT207.033957E-445.09
ATGCTCG207.033957E-445.035
CACGAAA406.8175723E-945.035
TCTAGCG351.2122473E-745.01
CCGTCCA207.033957E-445.045
TAGTTCG253.891362E-545.032
CGTTAGA351.2122473E-745.037
ATATCGA253.891362E-545.021