FastQCFastQC Report
Thu 26 May 2016
SRR1545188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545188_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences998053
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34710.3477771220566443No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26040.26090798785234853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG302.165214E-645.00000411
CCTAAGT302.165214E-645.00000420
GCCGATC302.165214E-645.0000049
GCGATAC302.165214E-645.0000049
ACCGTTA302.165214E-645.00000435
CGTTTAG302.165214E-645.00000430
GACAGTA351.2117198E-745.09
CTATGCG453.8380676E-1045.01
CCTTACG253.890435E-545.040
GATCGAC253.890435E-545.017
CGAAACA253.890435E-545.017
ACGTTCA207.0328393E-445.017
AACCCGC207.0328393E-445.036
CACGCGT207.0328393E-445.038
ATGTACG453.8380676E-1045.01
TACATGC207.0328393E-445.028
TAGTTAT207.0328393E-445.018
GTTACCT253.890435E-545.033
CCACTAG207.0328393E-445.020
ATTCACG253.890435E-545.012