FastQCFastQC Report
Thu 26 May 2016
SRR1545187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545187_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1458690
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82390.5648218607106377No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19360.13272182574775998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT207.0339104E-445.00000415
CGTCTTA207.0339104E-445.00000431
CATAGCG406.8175723E-945.0000041
TACCGGA207.0339104E-445.00000437
CTTAACG453.8562575E-1045.01
ATCACGC453.8562575E-1045.030
TACGCTA253.8913247E-545.040
TACGAAC253.8913247E-545.026
TCGAATT253.8913247E-545.039
CGTTTTT55400.042.888091
CGGTCTA4150.042.83132631
CGACGGT4200.042.3214328
TCGATAG1200.041.2500041
ACGTTAG556.184564E-1140.9090921
TCACGAC4350.040.3448325
GCGACGT1450.040.34482617
CGACGTC1450.040.34482618
CTACGGG3550.039.9295773
ACGGTCT4400.039.88636430
TAATGCG1200.039.3750041
Produced by FastQC (version 0.11.5)