##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545187_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1458690
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99556382781811	31.0	31.0	33.0	28.0	34.0
2	31.283758029464792	31.0	31.0	34.0	28.0	34.0
3	31.401195593306323	31.0	31.0	34.0	28.0	34.0
4	35.185848261111	37.0	35.0	37.0	32.0	37.0
5	35.03962322357732	37.0	35.0	37.0	32.0	37.0
6	35.07948913065833	37.0	35.0	37.0	32.0	37.0
7	35.70566878500573	37.0	35.0	37.0	35.0	37.0
8	35.705465177659406	37.0	35.0	37.0	35.0	37.0
9	37.394351095846275	39.0	37.0	39.0	35.0	39.0
10	36.5237569325902	39.0	35.0	39.0	32.0	39.0
11	36.24293715594129	38.0	35.0	39.0	31.0	39.0
12	35.613776059340914	37.0	35.0	39.0	30.0	39.0
13	35.32101268946795	37.0	35.0	39.0	30.0	39.0
14	36.20349423112519	38.0	35.0	40.0	30.0	41.0
15	36.45643899663396	38.0	35.0	40.0	30.0	41.0
16	36.58253775648013	38.0	35.0	40.0	31.0	41.0
17	36.50570443343	38.0	35.0	40.0	31.0	41.0
18	36.432702630442385	38.0	35.0	40.0	30.0	41.0
19	36.35542987200844	38.0	35.0	40.0	30.0	41.0
20	36.23688309373479	38.0	34.0	40.0	30.0	41.0
21	36.115106019784875	38.0	34.0	40.0	30.0	41.0
22	36.046543131165635	38.0	34.0	40.0	30.0	41.0
23	35.99944127950421	38.0	34.0	40.0	30.0	41.0
24	35.908409600394876	37.0	34.0	40.0	30.0	41.0
25	35.785000925487935	37.0	34.0	40.0	30.0	41.0
26	35.63507393620303	37.0	34.0	40.0	29.0	41.0
27	35.533853663218366	37.0	34.0	40.0	29.0	41.0
28	35.51621249203052	37.0	34.0	40.0	29.0	41.0
29	35.476343157216405	37.0	34.0	40.0	29.0	41.0
30	35.423202325374135	37.0	34.0	40.0	29.0	41.0
31	35.26765316825371	37.0	34.0	40.0	27.0	41.0
32	35.13053424648143	37.0	34.0	40.0	27.0	41.0
33	34.94190540827729	37.0	33.0	40.0	26.0	41.0
34	34.76324441793664	37.0	33.0	40.0	25.0	41.0
35	34.61491955110407	37.0	33.0	40.0	24.0	41.0
36	34.440854465307915	37.0	33.0	40.0	24.0	41.0
37	34.38521618712681	37.0	33.0	40.0	24.0	41.0
38	34.33560249264751	36.0	33.0	40.0	24.0	41.0
39	34.25976321219724	36.0	33.0	40.0	23.0	41.0
40	34.088353934009284	36.0	33.0	40.0	23.0	41.0
41	34.02161528494745	36.0	33.0	40.0	23.0	41.0
42	33.96372087283796	36.0	33.0	40.0	23.0	41.0
43	33.90443685772851	36.0	33.0	40.0	23.0	41.0
44	33.7843674804105	35.0	33.0	40.0	23.0	41.0
45	33.718925885554846	35.0	32.0	40.0	23.0	41.0
46	33.66134202606448	35.0	32.0	39.0	23.0	41.0
47	33.54737332812318	35.0	32.0	39.0	23.0	41.0
48	33.473562580123264	35.0	32.0	39.0	23.0	41.0
49	33.43322296032742	35.0	32.0	39.0	23.0	41.0
50	33.37716307097464	35.0	32.0	39.0	23.0	41.0
51	33.19791593827338	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	4.0
13	4.0
14	11.0
15	67.0
16	163.0
17	495.0
18	1045.0
19	2105.0
20	3480.0
21	5428.0
22	7922.0
23	11383.0
24	15367.0
25	21341.0
26	27272.0
27	32075.0
28	36024.0
29	40957.0
30	48339.0
31	57916.0
32	70189.0
33	87329.0
34	129696.0
35	168164.0
36	117438.0
37	142901.0
38	185813.0
39	245634.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.01654223995503	24.274931616724597	28.76896393339229	13.939562209928086
2	30.82896297362702	25.018749700073357	30.67005326697242	13.482234059327206
3	28.853971714346432	25.145164496911615	32.03552502587939	13.96533876286257
4	25.587959059155818	27.36990039007603	32.10853574097307	14.93360480979509
5	22.822738210311993	32.58972091397075	30.741692888824907	13.845847986892348
6	19.892780508538483	41.46391625362483	27.89996503712235	10.743338200714339
7	87.4555251629887	4.065908452104285	6.808300598482201	1.6702657864248058
8	88.8204484845992	2.931671568325004	6.533396403622429	1.7144835434533725
9	84.67556506180203	4.622366644043628	7.929443541808061	2.7726247523462835
10	43.075156476016154	27.274060972516434	15.305856624779768	14.344925926687644
11	38.333641829312604	23.162837888790627	22.55558069226498	15.947939589631794
12	35.64527075663781	21.032775983930787	25.88815992431565	17.433793335115755
13	24.930039967367982	30.910268802829936	26.416647814134603	17.743043415667483
14	20.068006224763316	33.451590125386474	28.52004195545318	17.96036169439703
15	18.167054000507303	23.394895419863026	39.80160280799896	18.63644777163071
16	20.78289424072284	19.934873071043196	38.35804728900589	20.924185399228072
17	21.097423030253175	20.50668750728393	28.945834961506556	29.450054500956334
18	24.169220327828395	22.777697797338707	32.08090821216297	20.97217366266993
19	27.827228540676906	25.152979728386427	25.529139159108517	21.490652571828146
20	29.91992815471416	23.941001857831342	25.30044080647704	20.838629180977453
21	25.061733473184844	27.01581556053719	27.778280511966212	20.144170454311748
22	23.96492743489021	23.19279627611076	27.09362510197506	25.748651187023974
23	21.451096531819648	27.722545571711603	26.451610691785092	24.374747204683654
24	21.359027620673345	24.60234868272217	34.41444035401627	19.624183342588214
25	21.623237288251786	25.256154494786422	31.072811906573705	22.047796310388087
26	20.331941673693517	29.55871364032111	26.723909809486596	23.385434876498774
27	19.51333045403753	29.889832658069913	28.42481952985213	22.17201735804043
28	18.717136608875087	27.367158203593632	33.50766783895139	20.408037348579892
29	19.347016843880468	24.870465966038022	33.28088901685759	22.50162817322392
30	21.710644482378026	27.14325867730635	30.09597652688371	21.050120313431915
31	27.54800540210737	25.52516298870905	26.325812886905375	20.60101872227821
32	27.82757131398721	25.127545948762247	25.956646031713387	21.08823670553716
33	27.391700772611046	25.897689022341964	25.570546174992632	21.140064030054365
34	21.336061808883315	26.601676847033985	28.33521858653998	23.727042757542726
35	21.734021622140414	25.92504233250382	29.057647615326083	23.283288430029682
36	27.307104319629254	25.778472465019988	25.5283165031638	21.38610671218696
37	21.335170598276537	30.988078344267798	26.417059142106957	21.259691915348704
38	22.435747142984457	29.881880317270976	25.022520206486643	22.659852333257923
39	22.11744784704084	28.890442794562244	25.781763088798854	23.210346269598066
40	24.812400167273374	25.552721962857085	26.561709479053125	23.073168390816416
41	20.587307789866248	24.153041427582284	27.911824993658698	27.34782578889277
42	21.85789989648246	26.067773138912308	25.59152390158293	26.482803063022303
43	22.141579088085887	25.77881523833028	26.876375377907575	25.20323029567626
44	22.191281218079236	27.1607401161316	27.52572513693794	23.122253528851232
45	19.611226511458913	31.216228259602797	25.0771582721483	24.095386956789998
46	21.913360618088834	29.556519891135196	26.78547189601629	21.74464759475968
47	22.032440066086696	27.931500181669854	26.852381246186646	23.183678506056804
48	22.1031884773324	26.243821511081862	29.226017865344932	22.426972146240807
49	22.281019270715504	25.417463614613112	29.754162981853582	22.5473541328178
50	21.455004147557055	29.01733747403492	27.4985089361002	22.029149442307823
51	21.39131686650351	30.446565068657495	25.180812921182707	22.981305143656293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	741.0
1	1196.5
2	1652.0
3	7507.0
4	13362.0
5	9605.5
6	5849.0
7	6014.0
8	6179.0
9	6588.0
10	6997.0
11	7166.0
12	7335.0
13	7069.0
14	6803.0
15	6403.0
16	6003.0
17	5672.0
18	5341.0
19	5499.0
20	5657.0
21	5498.5
22	5340.0
23	5752.5
24	6165.0
25	6493.5
26	9185.0
27	11548.0
28	13417.0
29	15286.0
30	18045.5
31	20805.0
32	25526.0
33	30247.0
34	35868.5
35	41490.0
36	41760.5
37	42031.0
38	48667.5
39	55304.0
40	67930.5
41	80557.0
42	91319.0
43	102081.0
44	108918.5
45	115756.0
46	111912.0
47	108068.0
48	108670.5
49	109273.0
50	104795.0
51	100317.0
52	97303.0
53	94289.0
54	89041.0
55	83793.0
56	81661.5
57	79530.0
58	73012.0
59	66494.0
60	62945.5
61	59397.0
62	52879.5
63	46362.0
64	40065.0
65	33768.0
66	29933.0
67	26098.0
68	22563.5
69	19029.0
70	16073.0
71	13117.0
72	11070.5
73	9024.0
74	7003.0
75	3940.0
76	2898.0
77	2220.5
78	1543.0
79	1156.0
80	769.0
81	503.5
82	238.0
83	234.0
84	230.0
85	165.0
86	100.0
87	55.0
88	10.0
89	7.5
90	5.0
91	4.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1458690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.73428808059648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.28155212044479	16.02377964980934
2	7.086828601583363	2.9388069160608037
3	2.813379712701472	1.7500027632977426
4	1.68975672508501	1.401436108961515
5	1.0809894329809724	1.1206773157754064
6	0.8271601113628291	1.02903456226651
7	0.6493229848439437	0.9424274877574977
8	0.552199163342112	0.9159565224479762
9	0.42774702479586296	0.7982127033961922
>10	5.376691587644396	25.841584202267633
>50	1.358307674443255	20.048937560695165
>100	0.8510954440107087	25.861817467457097
>500	0.003975533409156423	0.5564702126573409
>1k	6.625889015260705E-4	0.20490688116983677
>5k	3.3129445076303523E-4	0.5659496459799817
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8239	0.5648218607106377	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1936	0.13272182574775998	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020977726590296772	0.0
2	0.0	0.0	0.0	0.0848706716300242	0.0
3	0.0	0.0	0.0	0.12127319718377448	0.0
4	0.0	0.0	0.0	0.16734193008795564	0.0
5	0.0	0.0	0.0	0.28820379929937134	0.0
6	0.0	0.0	0.0	0.43319690955583434	0.0
7	0.0	0.0	0.0	0.5336294894734317	0.0
8	0.0	0.0	0.0	0.7912579094941351	0.0
9	0.0	0.0	0.0	0.9078008349957839	0.0
10	0.0	0.0	0.0	1.1046212697694506	0.0
11	0.0	0.0	0.0	1.2569497288663116	0.0
12	0.0	0.0	0.0	1.39680123946829	0.0
13	0.0	0.0	0.0	1.4530160623573205	0.0
14	0.0	0.0	0.0	1.4796838258985803	0.0
15	0.0	0.0	0.0	1.513961156928477	0.0
16	0.0	0.0	0.0	1.5882058559392331	0.0
17	0.0	0.0	0.0	1.681440196340552	0.0
18	0.0	0.0	0.0	1.8158073339777472	0.0
19	0.0	0.0	0.0	1.872570594163256	0.0
20	0.0	0.0	0.0	1.9374918591338803	0.0
21	0.0	0.0	0.0	2.0172209311094202	0.0
22	0.0	0.0	0.0	2.0983896509882154	0.0
23	0.0	0.0	0.0	2.198068129623155	0.0
24	0.0	0.0	0.0	2.272381383295971	0.0
25	0.0	0.0	0.0	2.3373026482665953	0.0
26	0.0	0.0	0.0	2.4049660997196116	0.0
27	0.0	0.0	0.0	2.479759236026846	0.0
28	0.0	0.0	0.0	2.5539353803755422	0.0
29	0.0	0.0	0.0	2.6445646436185895	0.0
30	0.0	0.0	0.0	2.7519212444042256	0.0
31	0.0	0.0	0.0	2.8431674996058107	0.0
32	6.855466205979338E-5	0.0	0.0	2.9231707902295896	0.0
33	6.855466205979338E-5	0.0	0.0	3.0087955631422716	0.0
34	6.855466205979338E-5	0.0	0.0	3.104909199350102	0.0
35	6.855466205979338E-5	0.0	0.0	3.2211779062035113	0.0
36	6.855466205979338E-5	0.0	0.0	3.313863809308352	0.0
37	6.855466205979338E-5	0.0	0.0	3.4169700210462812	0.0
38	6.855466205979338E-5	0.0	0.0	3.545921340380753	0.0
39	6.855466205979338E-5	0.0	0.0	3.7469236095400666	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGAT	20	7.0339104E-4	45.000004	15
CGTCTTA	20	7.0339104E-4	45.000004	31
CATAGCG	40	6.8175723E-9	45.000004	1
TACCGGA	20	7.0339104E-4	45.000004	37
CTTAACG	45	3.8562575E-10	45.0	1
ATCACGC	45	3.8562575E-10	45.0	30
TACGCTA	25	3.8913247E-5	45.0	40
TACGAAC	25	3.8913247E-5	45.0	26
TCGAATT	25	3.8913247E-5	45.0	39
CGTTTTT	5540	0.0	42.88809	1
CGGTCTA	415	0.0	42.831326	31
CGACGGT	420	0.0	42.32143	28
TCGATAG	120	0.0	41.250004	1
ACGTTAG	55	6.184564E-11	40.909092	1
TCACGAC	435	0.0	40.34483	25
GCGACGT	145	0.0	40.344826	17
CGACGTC	145	0.0	40.344826	18
CTACGGG	355	0.0	39.929577	3
ACGGTCT	440	0.0	39.886364	30
TAATGCG	120	0.0	39.375004	1
>>END_MODULE