##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545186_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1607798
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9826924775376	31.0	31.0	33.0	28.0	34.0
2	31.253763221499216	31.0	31.0	34.0	28.0	34.0
3	31.373733516275056	31.0	31.0	34.0	28.0	34.0
4	35.145143855136034	35.0	35.0	37.0	32.0	37.0
5	34.986736517895906	35.0	35.0	37.0	32.0	37.0
6	35.03752648031656	37.0	35.0	37.0	32.0	37.0
7	35.68480244408813	37.0	35.0	37.0	35.0	37.0
8	35.678190917018185	37.0	35.0	37.0	35.0	37.0
9	37.348719179896975	39.0	37.0	39.0	35.0	39.0
10	36.53499693369441	39.0	35.0	39.0	32.0	39.0
11	36.19825873648306	38.0	35.0	39.0	31.0	39.0
12	35.33594332123812	37.0	35.0	39.0	30.0	39.0
13	34.923024534176555	37.0	34.0	39.0	27.0	39.0
14	35.85331677238061	38.0	34.0	40.0	29.0	41.0
15	36.21605948010882	38.0	34.0	40.0	30.0	41.0
16	36.370739981017515	38.0	35.0	40.0	31.0	41.0
17	36.29934108638025	38.0	35.0	40.0	31.0	41.0
18	36.2589890023498	37.0	35.0	40.0	31.0	41.0
19	36.182431499479414	37.0	35.0	40.0	30.0	41.0
20	36.03671170134557	37.0	34.0	40.0	30.0	41.0
21	35.901343949924055	37.0	34.0	40.0	30.0	41.0
22	35.80699689886416	37.0	34.0	40.0	30.0	41.0
23	35.76121565022472	37.0	34.0	40.0	30.0	41.0
24	35.66018305782194	36.0	34.0	40.0	30.0	41.0
25	35.5713161728028	36.0	34.0	40.0	29.0	41.0
26	35.393437484062055	36.0	34.0	40.0	29.0	41.0
27	35.31304430034121	36.0	34.0	40.0	29.0	41.0
28	35.33874591211085	36.0	34.0	40.0	29.0	41.0
29	35.33608699600323	36.0	34.0	40.0	29.0	41.0
30	35.24461717205769	36.0	34.0	40.0	29.0	41.0
31	35.08740588058948	36.0	34.0	40.0	28.0	41.0
32	34.91276889260964	36.0	34.0	40.0	27.0	41.0
33	34.70742095711029	36.0	33.0	40.0	26.0	41.0
34	34.57034963347386	36.0	33.0	40.0	25.0	41.0
35	34.41382437345985	36.0	33.0	40.0	25.0	41.0
36	34.2099890657906	35.0	33.0	40.0	24.0	41.0
37	34.169109552319384	35.0	33.0	40.0	24.0	41.0
38	34.14585041155667	35.0	33.0	40.0	24.0	41.0
39	34.1045560449758	35.0	33.0	40.0	24.0	41.0
40	33.94221413386507	35.0	33.0	40.0	23.0	41.0
41	33.90692985064044	35.0	33.0	40.0	23.0	41.0
42	33.83622693895626	35.0	33.0	40.0	23.0	41.0
43	33.74181333724759	35.0	33.0	40.0	23.0	41.0
44	33.614691646587445	35.0	33.0	39.0	23.0	41.0
45	33.57117249803769	35.0	32.0	39.0	23.0	41.0
46	33.46269680643962	35.0	32.0	39.0	23.0	41.0
47	33.377345910369336	35.0	32.0	39.0	23.0	41.0
48	33.33262822817294	35.0	32.0	39.0	23.0	41.0
49	33.33764875935908	35.0	32.0	39.0	23.0	41.0
50	33.240641548254196	35.0	32.0	39.0	23.0	41.0
51	33.05103750595535	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	6.0
13	5.0
14	16.0
15	63.0
16	209.0
17	562.0
18	1284.0
19	2338.0
20	3933.0
21	6199.0
22	9139.0
23	12851.0
24	18023.0
25	23322.0
26	29707.0
27	34765.0
28	39444.0
29	45602.0
30	54767.0
31	66465.0
32	80659.0
33	101447.0
34	158784.0
35	221258.0
36	121392.0
37	141188.0
38	181725.0
39	252473.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.873781407863426	23.657511702340717	29.967072978073116	13.50163391172274
2	29.251746799038187	26.74260074959665	30.778928696266572	13.226723755098588
3	28.561734745285168	26.319226669021855	31.644522508424565	13.474516077268413
4	24.794532646514053	28.376699062941984	31.857982159450376	14.970786131093583
5	22.56116750984887	32.06329402076629	30.513596857316656	14.861941612068183
6	19.35417260128449	42.63010651835616	27.31586928208643	10.699851598272918
7	86.5876807907461	4.934823902007591	6.643434063234312	1.8340612440119963
8	87.20063092502915	4.245682604406772	5.903913302541737	2.649773168022351
9	82.70230464274742	5.938183776817735	8.064881284838021	3.294630295596835
10	46.971261315165215	28.054768074098863	13.424820779724817	11.54914983101111
11	42.29666910893035	22.59873441812964	21.13095052985512	13.97364594308489
12	39.10049645540049	21.917740910238724	23.78389573814621	15.197866896214574
13	22.392116422585424	39.47106539503097	22.985287952839847	15.151530229543761
14	16.10202276654157	41.718113842659335	24.95033580089041	17.229527589908685
15	14.042373482240928	24.54114260622292	44.67283825455685	16.743645656979297
16	15.610916296698962	21.08218818533174	42.353890227503705	20.953005290465594
17	15.535969070741473	21.14612656565066	28.354494781060808	34.96340958254706
18	20.145876534241243	24.341117478688243	34.00663516187979	21.506370825190725
19	27.04263844089867	26.394173894979346	24.431240740441275	22.13194692368071
20	29.516767653648035	23.576780167657876	24.99480656152079	21.9116456171733
21	20.85541840455082	31.14638779249632	26.910034718291726	21.088159084661132
22	22.301495585888276	26.36338644531216	24.261878668837753	27.07323929996181
23	18.645377093391087	31.56204946143732	23.846279196764765	25.946294248406826
24	19.00213832832234	25.568448275218653	37.23770025836579	18.191713138093217
25	16.96550188518707	27.538969447654495	33.42913724236502	22.066391424793412
26	15.950573392926227	34.90320301430901	26.175365313304283	22.97085827946048
27	17.255401487002718	34.44157785990529	26.7518058860628	21.551214767029194
28	15.665960524891808	29.620076651420142	35.787953461815476	18.926009361872573
29	16.05382019383032	24.788375156580617	35.26929377944244	23.88851087014662
30	17.92414221189478	32.74030692910428	29.747020459037763	19.58853039996318
31	27.984920991318564	28.319913322444734	23.411336498739267	20.283829187497435
32	27.54593549687212	28.234641416396837	24.553768570429867	19.665654516301174
33	26.407421827866433	28.8001353403848	23.42844063744326	21.364002194305503
34	18.251173343915095	28.845228069695324	26.985106337985243	25.91849224840434
35	18.724367115769518	25.802806073897344	30.38298343448617	25.089843375846964
36	27.482681282101357	26.725185626552587	25.069256212534164	20.722876878811892
37	18.01053366156694	33.02417343472252	28.82333477215421	20.14195813155633
38	18.237552229819915	32.317430423473596	24.976458485456508	24.468558861249985
39	18.93546328581078	31.9180643339524	26.987718606441852	22.158753773794967
40	23.23401322802989	27.626107259742827	24.6091859798308	24.530693532396484
41	17.194386359480482	24.402008212474453	27.85306363112779	30.550541796917273
42	20.283393809421334	25.585303626450585	25.20229531321721	28.92900725091087
43	21.46177567082432	25.576036293116424	26.36089857059158	26.601289465467676
44	19.18704961692949	29.202673470174734	27.896103863793837	23.714173049101937
45	17.009475070873332	36.262142383558135	22.674738990843377	24.053643554725156
46	21.08411628824019	33.302815403427545	24.803613389244173	20.809454919088093
47	19.764734127048296	28.997299411990813	26.32911597103616	24.90885048992473
48	20.749497138322106	25.43130418124665	30.714617134739562	23.104581545691687
49	20.848887733409295	24.70963392167424	31.05999634282416	23.3814820020923
50	19.523348082283967	31.417752727643645	27.12063331338887	21.938265876683513
51	17.925697133595143	32.066901439111135	24.95238829753489	25.055013129758834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1844.0
1	1985.5
2	2127.0
3	5908.5
4	9690.0
5	7455.5
6	5221.0
7	5875.5
8	6530.0
9	7253.0
10	7976.0
11	8562.0
12	9148.0
13	9261.5
14	9375.0
15	9050.5
16	8726.0
17	8562.0
18	8398.0
19	7670.0
20	6942.0
21	7206.0
22	7470.0
23	7506.5
24	7543.0
25	8543.5
26	11130.5
27	12717.0
28	14923.0
29	17129.0
30	21529.0
31	25929.0
32	30347.5
33	34766.0
34	38825.0
35	42884.0
36	45440.5
37	47997.0
38	56002.0
39	64007.0
40	85040.5
41	106074.0
42	123970.0
43	141866.0
44	150070.5
45	158275.0
46	155441.5
47	152608.0
48	149699.5
49	146791.0
50	132343.5
51	117896.0
52	106900.5
53	95905.0
54	86835.0
55	77765.0
56	71232.5
57	64700.0
58	56087.0
59	47474.0
60	43226.5
61	38979.0
62	37005.0
63	35031.0
64	31516.0
65	28001.0
66	22415.5
67	16830.0
68	14338.5
69	11847.0
70	9657.0
71	7467.0
72	6978.0
73	6489.0
74	5306.0
75	2833.0
76	1543.0
77	1416.0
78	1289.0
79	872.0
80	455.0
81	342.0
82	229.0
83	192.0
84	155.0
85	89.5
86	24.0
87	16.0
88	8.0
89	6.0
90	4.0
91	4.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1607798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.085072668844724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75658889788929	16.01916883833281
2	8.264709987168871	3.3199460135842704
3	3.0009977301375224	1.8082577246655063
4	1.5129683839267207	1.21552319747331
5	0.9316137326088867	0.9355764759371582
6	0.624555012646816	0.7526539688821521
7	0.45290409607146853	0.6367628177129016
8	0.33780572127482267	0.5427881967805056
9	0.2988825594491968	0.5402770133388679
>10	2.769514074903354	12.686179810765614
>50	0.8290264582124436	11.950947922568863
>100	1.2095781571210913	47.27783906439183
>500	0.00899429911705255	1.2649318206021223
>1k	0.0012405929816624206	0.3515222231684121
>5k	6.202964908312103E-4	0.6976249117956843
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5685	0.3535891946625136	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5514	0.3429535302320316	No Hit
GTCTGAGGGTCAAGAAGAAGGAGCTACTCCCCACCACCAGCACCCAAAGCT	1919	0.11935578971985286	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0889415212607554	0.0
2	0.0	0.0	0.0	0.30986479644831005	0.0
3	0.0	0.0	0.0	0.42760346759978557	0.0
4	0.0	0.0	0.0	0.6382020626969308	0.0
5	0.0	0.0	0.0	1.0596480403632795	0.0
6	0.0	0.0	0.0	1.52823924398463	0.0
7	0.0	0.0	0.0	1.8361137406564756	0.0
8	0.0	0.0	0.0	2.6557440673517445	0.0
9	0.0	0.0	0.0	3.0351449622402815	0.0
10	0.0	0.0	0.0	3.6755239153177204	0.0
11	0.0	0.0	0.0	4.10387374533368	0.0
12	0.0	0.0	0.0	4.535768796826467	0.0
13	0.0	0.0	0.0	4.69797822860832	0.0
14	0.0	0.0	0.0	4.758309190582399	0.0
15	0.0	0.0	0.0	4.843581096630298	0.0
16	0.0	0.0	0.0	5.036329190607278	0.0
17	0.0	0.0	0.0	5.257252465794832	0.0
18	0.0	0.0	0.0	5.627697011689279	0.0
19	6.219686801451426E-5	0.0	0.0	5.754889606778961	0.0
20	6.219686801451426E-5	0.0	0.0	5.88699575444179	0.0
21	6.219686801451426E-5	0.0	0.0	6.031852260047593	0.0
22	1.243937360290285E-4	0.0	0.0	6.171297638136134	0.0
23	1.243937360290285E-4	0.0	0.0	6.335372975958423	0.0
24	1.243937360290285E-4	0.0	0.0	6.458211790287089	0.0
25	1.243937360290285E-4	0.0	0.0	6.554927920049658	0.0
26	1.243937360290285E-4	0.0	0.0	6.652328215360387	0.0
27	1.243937360290285E-4	0.0	0.0	6.759990993893512	0.0
28	1.243937360290285E-4	0.0	0.0	6.868462331710824	0.0
29	1.243937360290285E-4	0.0	0.0	6.985454640446125	0.0
30	1.243937360290285E-4	0.0	0.0	7.154070349633474	0.0
31	1.243937360290285E-4	0.0	0.0	7.289908309377173	0.0
32	1.243937360290285E-4	0.0	0.0	7.40932629596504	0.0
33	1.243937360290285E-4	0.0	0.0	7.528308904476806	0.0
34	1.243937360290285E-4	0.0	0.0	7.649654993973123	0.0
35	1.243937360290285E-4	0.0	0.0	7.804587392197279	0.0
36	1.243937360290285E-4	0.0	0.0	7.923321213236986	0.0
37	1.243937360290285E-4	0.0	0.0	8.051384564478871	0.0
38	1.243937360290285E-4	0.0	0.0	8.177955190888408	0.0
39	1.243937360290285E-4	0.0	0.0	8.308568613718888	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTAAG	125	0.0	45.000004	1
TCGTTGC	35	1.2123382E-7	45.0	34
TAGCGAT	25	3.891503E-5	45.0	11
TGAACGT	40	6.8175723E-9	45.0	10
CGCGATA	20	7.034127E-4	45.0	11
CGTTCCA	20	7.034127E-4	45.0	33
AACGGTC	20	7.034127E-4	45.0	38
TCAATCG	35	1.2123382E-7	45.0	21
GTATCGC	25	3.891503E-5	45.0	9
GTATACG	50	2.1827873E-11	45.0	1
AGTAGTA	20	7.034127E-4	45.0	10
GTCCAAT	45	3.8562575E-10	45.0	36
TATACGT	50	2.1827873E-11	45.0	43
CAATCGA	35	1.2123382E-7	45.0	22
TAAGCGA	35	1.2123382E-7	45.0	45
GCCACTC	20	7.034127E-4	45.0	35
AATACGG	80	0.0	45.0	2
ATCGCCT	40	6.8175723E-9	45.0	27
TACACGG	75	0.0	45.0	2
ATTCTCG	25	3.891503E-5	45.0	30
>>END_MODULE