FastQCFastQC Report
Thu 26 May 2016
SRR1545185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545185_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2099657
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74720.3558676488588374No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36750.1750285880027071No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27130.12921158074866515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCACG750.045.0000041
GCGCTAA351.2125747E-745.00000410
ACAACGT207.034623E-445.035
GTCGACC207.034623E-445.09
TAGAACG950.045.01
CGCGATC207.034623E-445.012
TATGCGA207.034623E-445.021
CCGTAGT207.034623E-445.028
GGGCGTA253.8919115E-545.07
CGTAAGT207.034623E-445.014
ATGCGAT207.034623E-445.022
CCGAATA453.8562575E-1045.021
ATCGGCG207.034623E-445.036
ACTACGT302.1663636E-644.99999643
GTCCGTT302.1663636E-644.9999969
ATACGTA302.1663636E-644.99999644
CGTTTTT57450.042.767621
TCACGAC7800.042.69230725
CGACGGT7650.042.6470628
GTATGCG2350.042.127661
Produced by FastQC (version 0.11.5)