##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545185_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2099657
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.006873979892905	31.0	31.0	33.0	28.0	34.0
2	31.285193248230545	31.0	31.0	34.0	28.0	34.0
3	31.404679430973726	31.0	31.0	34.0	28.0	34.0
4	35.18303132368763	37.0	35.0	37.0	32.0	37.0
5	35.03855820260166	37.0	35.0	37.0	32.0	37.0
6	35.0947421412164	37.0	35.0	37.0	32.0	37.0
7	35.716465117874016	37.0	35.0	37.0	35.0	37.0
8	35.72128876287889	37.0	35.0	37.0	35.0	37.0
9	37.41533117075789	39.0	37.0	39.0	35.0	39.0
10	36.548417670124216	39.0	35.0	39.0	32.0	39.0
11	36.2718158251562	38.0	35.0	39.0	31.0	39.0
12	35.56411166204766	37.0	35.0	39.0	30.0	39.0
13	35.225906898126695	37.0	35.0	39.0	30.0	39.0
14	36.18746728632343	38.0	35.0	40.0	30.0	41.0
15	36.470133931399275	38.0	35.0	40.0	30.0	41.0
16	36.60022232202688	38.0	35.0	40.0	31.0	41.0
17	36.52360742730836	38.0	35.0	40.0	31.0	41.0
18	36.47081261367928	38.0	35.0	40.0	31.0	41.0
19	36.42614198414312	38.0	35.0	40.0	31.0	41.0
20	36.30743783389382	38.0	35.0	40.0	30.0	41.0
21	36.16602902283564	38.0	34.0	40.0	30.0	41.0
22	36.06833163702452	38.0	34.0	40.0	30.0	41.0
23	36.028520848881506	38.0	34.0	40.0	30.0	41.0
24	35.95772499984521	37.0	34.0	40.0	30.0	41.0
25	35.86242086207414	37.0	34.0	40.0	30.0	41.0
26	35.70027580695323	37.0	34.0	40.0	29.0	41.0
27	35.6164625936522	37.0	34.0	40.0	29.0	41.0
28	35.60869894463715	37.0	34.0	40.0	29.0	41.0
29	35.608136948082475	37.0	34.0	40.0	29.0	41.0
30	35.54699886695779	37.0	34.0	40.0	29.0	41.0
31	35.41517543103469	37.0	34.0	40.0	29.0	41.0
32	35.2996951406825	37.0	34.0	40.0	28.0	41.0
33	35.12889867249746	37.0	34.0	40.0	27.0	41.0
34	34.983253455207205	37.0	34.0	40.0	27.0	41.0
35	34.877267572751165	37.0	33.0	40.0	26.0	41.0
36	34.70543093467171	37.0	33.0	40.0	25.0	41.0
37	34.666990846600186	37.0	33.0	40.0	25.0	41.0
38	34.63731885731812	37.0	33.0	40.0	25.0	41.0
39	34.58870282146084	36.0	33.0	40.0	25.0	41.0
40	34.463875290106905	36.0	33.0	40.0	24.0	41.0
41	34.402962483872365	36.0	33.0	40.0	24.0	41.0
42	34.33501662414385	36.0	33.0	40.0	24.0	41.0
43	34.26856719930922	36.0	33.0	40.0	24.0	41.0
44	34.148316605998026	36.0	33.0	40.0	24.0	41.0
45	34.08754763278002	35.0	33.0	40.0	24.0	41.0
46	33.987758476741675	35.0	33.0	40.0	23.0	41.0
47	33.90212353732062	35.0	33.0	40.0	23.0	41.0
48	33.85507632913376	35.0	33.0	39.0	23.0	41.0
49	33.83247073212434	35.0	33.0	39.0	24.0	41.0
50	33.76747583057614	35.0	32.0	39.0	24.0	41.0
51	33.58673726232428	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	5.0
14	24.0
15	74.0
16	229.0
17	604.0
18	1358.0
19	2512.0
20	4238.0
21	6651.0
22	9889.0
23	14231.0
24	19903.0
25	27518.0
26	35549.0
27	42667.0
28	48538.0
29	56761.0
30	67681.0
31	81958.0
32	100553.0
33	126198.0
34	191115.0
35	258561.0
36	164975.0
37	199456.0
38	262344.0
39	375808.0
40	253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.47937305950448	24.071741241545645	29.225059140612014	13.223826558337862
2	30.2477023628145	25.47473230151401	31.12551240512141	13.152052930550084
3	28.473269681667052	25.80778670039916	31.735612054730844	13.983331563202942
4	26.098643730856992	28.602719396549052	30.68110648548787	14.617530387106084
5	22.77100497843219	32.42177174652812	30.435256806230733	14.371966468808953
6	19.598724934596458	42.853523218316134	27.07275521668539	10.474996630402012
7	88.51845801480908	4.173110179424544	5.689453086861331	1.6189787189050402
8	89.51223938005111	3.1909973867160204	5.4263624963505945	1.8704007368822624
9	85.03655597080856	5.427124525577272	6.959851061387646	2.576468442226516
10	42.95430158354436	32.03408937745546	13.106569311082714	11.905039727917464
11	39.0620468009775	22.289545387651412	23.411776304415437	15.23663150695566
12	37.5367976769539	20.98404644187122	25.40543526871294	16.07372061246194
13	23.197407957585455	35.51237178262926	25.350473910738753	15.939746349046535
14	18.158727830307523	36.58835705069923	26.867150205962208	18.385764913031032
15	15.790340993790892	23.99544306522446	41.2996979982921	18.914517942692544
16	18.104004606466674	20.659660125439537	39.720821067441015	21.515514200652774
17	17.852439708009452	21.115210722513247	28.98206707095492	32.05028249852238
18	21.48203254150559	23.482549768843196	33.48699335177127	21.548424337879947
19	27.08890071092564	26.773515864734097	24.1509922811202	21.98659114322006
20	29.257493009572517	23.93133735653014	25.72253468066451	21.088634953232837
21	22.501770527281362	28.5149907818277	27.681616568801477	21.301622122089466
22	22.93045959411466	24.704273126515425	24.712036299262213	27.6532309801077
23	20.01345934121621	29.225440155225353	25.486972395967534	25.274128107590904
24	20.19334586553899	24.262058040908588	36.43280783480349	19.11178825874893
25	19.362972142592813	25.780020260452062	31.958934244974298	22.898073351980823
26	18.004559792385137	32.67547985218538	25.80359553965243	23.516364815777052
27	18.504260457779534	31.96750707377443	27.86040767611091	21.66782479233513
28	17.019922777863243	28.440502424919877	34.55673950554781	19.98283529166907
29	17.974888279371346	24.232481781548127	34.050132950286645	23.742496988793885
30	20.1315262445247	29.98599295027712	29.512439412723126	20.37004139247506
31	26.937685536256634	27.21930296234099	24.9418833647591	20.901128136643273
32	27.355134671996428	26.569292031984272	25.33737653340522	20.738196762614084
33	26.3383971763007	27.321319625062568	24.245150517441658	22.09513268119507
34	18.962716291279957	27.331083124529385	27.53716440351924	26.169036180671412
35	20.386329767195306	25.300513369564648	30.90390478063798	23.409252082602062
36	26.857958228415402	26.141888889470994	26.22599786536563	20.774155016747976
37	19.46279797128769	32.49083064519586	27.404571318077192	20.64180006543926
38	19.615870592196725	31.430228842139453	26.195754830431827	22.758145735231995
39	20.499014839090385	31.46566320118	25.913041987334122	22.122279972395493
40	23.853419868102264	26.39469208542157	25.869653948240117	23.882234098236047
41	18.900420401998993	24.25319945114845	28.002240365926433	28.84413978092612
42	20.25664191817997	26.104168442750407	25.339186352818576	28.30000328625104
43	20.48958472741024	26.697789210332928	26.18413388472498	26.628492177531854
44	20.546594038931122	27.79820704048328	28.124546056808324	23.530652863777274
45	19.048063564667945	33.45827437529082	24.064216202932194	23.42944585710904
46	21.095350335792943	31.940407409400677	26.257812585579455	20.706429669226925
47	19.57376847742274	28.35396448086521	27.67923522746811	24.393031814243944
48	20.311317515194148	26.013153576989	30.72106539306182	22.95446351475503
49	20.690855696906684	25.293226465084533	31.133751846134867	22.882165991873908
50	19.95087769097524	30.17959600068011	28.468506999000315	21.401019309344335
51	19.015820203014112	30.822653414343392	26.42550664227538	23.736019740367116
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1216.0
1	1937.0
2	2658.0
3	8002.0
4	13346.0
5	10019.0
6	6692.0
7	7086.5
8	7481.0
9	7824.0
10	8167.0
11	8435.5
12	8704.0
13	8642.0
14	8580.0
15	8057.0
16	7534.0
17	7110.5
18	6687.0
19	6990.5
20	7294.0
21	7247.5
22	7201.0
23	7274.0
24	7347.0
25	9874.5
26	16480.5
27	20559.0
28	23669.5
29	26780.0
30	30497.5
31	34215.0
32	40719.0
33	47223.0
34	53457.5
35	59692.0
36	64926.5
37	70161.0
38	79597.5
39	89034.0
40	112273.5
41	135513.0
42	158396.0
43	181279.0
44	184045.5
45	186812.0
46	184233.0
47	181654.0
48	172194.5
49	162735.0
50	155231.0
51	147727.0
52	139749.0
53	131771.0
54	120286.5
55	108802.0
56	101814.0
57	94826.0
58	89675.0
59	84524.0
60	74751.0
61	64978.0
62	58155.5
63	51333.0
64	44490.0
65	37647.0
66	31846.5
67	26046.0
68	22152.0
69	18258.0
70	15452.5
71	12647.0
72	10765.5
73	8884.0
74	7554.0
75	4545.5
76	2867.0
77	1974.5
78	1082.0
79	844.5
80	607.0
81	431.0
82	255.0
83	185.0
84	115.0
85	97.5
86	80.0
87	47.5
88	15.0
89	8.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2099657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.50289786197846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3718245992383	13.892369390793375
2	7.731004721407858	2.7062997201854997
3	2.9108493626098375	1.5284489725609525
4	1.525564673495289	1.0680721064812224
5	0.9556832584257269	0.8363613230314133
6	0.6794359968629938	0.7135259314106699
7	0.45946454226159733	0.5629372668083797
8	0.36582091945015327	0.5122340951128865
9	0.29971654714448404	0.47213173009932796
>10	3.2428363885661864	13.139847787339932
>50	1.0115241719530081	12.753982123816723
>100	1.4282849697300097	49.10715708659063
>500	0.016081834582154293	1.8304703514114764
>1k	0.0016354408049648436	0.5196860284071416
>5k	2.725734674941406E-4	0.3564760859504418
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7472	0.3558676488588374	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3675	0.1750285880027071	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2713	0.12921158074866515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033862673760523744	0.0
2	4.7626826667403294E-5	0.0	0.0	0.12068637877519994	0.0
3	4.7626826667403294E-5	0.0	0.0	0.17159945648265407	0.0
4	4.7626826667403294E-5	0.0	0.0	0.2560418201639601	0.0
5	4.7626826667403294E-5	0.0	0.0	0.44288186118018324	0.0
6	4.7626826667403294E-5	0.0	0.0	0.6477248426766848	0.0
7	4.7626826667403294E-5	0.0	0.0	0.7859378936654892	0.0
8	4.7626826667403294E-5	0.0	0.0	1.1949094542584813	0.0
9	4.7626826667403294E-5	0.0	0.0	1.3725575177278955	0.0
10	4.7626826667403294E-5	0.0	0.0	1.66703418701245	0.0
11	4.7626826667403294E-5	0.0	0.0	1.8860223360291706	0.0
12	4.7626826667403294E-5	0.0	0.0	2.097628326912443	0.0
13	4.7626826667403294E-5	0.0	0.0	2.1816896759804103	0.0
14	4.7626826667403294E-5	0.0	0.0	2.2158857375276058	0.0
15	4.7626826667403294E-5	0.0	0.0	2.2633220568883394	0.0
16	4.7626826667403294E-5	0.0	0.0	2.374911711770065	0.0
17	4.7626826667403294E-5	0.0	0.0	2.502551607238706	0.0
18	9.525365333480659E-5	0.0	0.0	2.69415433092167	0.0
19	9.525365333480659E-5	0.0	0.0	2.7688331951361578	0.0
20	9.525365333480659E-5	0.0	0.0	2.8535613197774685	0.0
21	9.525365333480659E-5	0.0	0.0	2.9586260994057603	0.0
22	9.525365333480659E-5	0.0	0.0	3.067882039780783	0.0
23	9.525365333480659E-5	0.0	0.0	3.194807532849413	0.0
24	9.525365333480659E-5	0.0	0.0	3.2883942472508605	0.0
25	9.525365333480659E-5	0.0	0.0	3.3635970065586904	0.0
26	9.525365333480659E-5	0.0	0.0	3.4407048389332164	0.0
27	9.525365333480659E-5	0.0	0.0	3.5205750272544516	0.0
28	9.525365333480659E-5	0.0	0.0	3.6042077348824115	0.0
29	9.525365333480659E-5	0.0	0.0	3.7006996857105707	0.0
30	9.525365333480659E-5	0.0	0.0	3.8245294350458194	0.0
31	9.525365333480659E-5	0.0	0.0	3.928165409874089	0.0
32	1.428804800022099E-4	0.0	0.0	4.028943775102315	0.0
33	1.428804800022099E-4	0.0	0.0	4.132579749930584	0.0
34	1.428804800022099E-4	0.0	0.0	4.240787900118924	0.0
35	1.9050730666961318E-4	0.0	0.0	4.3832397386811275	0.0
36	2.3813413333701648E-4	0.0	0.0	4.49397211068284	0.0
37	2.3813413333701648E-4	0.0	0.0	4.603418558364533	0.0
38	2.3813413333701648E-4	0.0	0.0	4.721818849459697	0.0
39	2.3813413333701648E-4	0.0	0.0	4.845315211008274	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCACG	75	0.0	45.000004	1
GCGCTAA	35	1.2125747E-7	45.000004	10
ACAACGT	20	7.034623E-4	45.0	35
GTCGACC	20	7.034623E-4	45.0	9
TAGAACG	95	0.0	45.0	1
CGCGATC	20	7.034623E-4	45.0	12
TATGCGA	20	7.034623E-4	45.0	21
CCGTAGT	20	7.034623E-4	45.0	28
GGGCGTA	25	3.8919115E-5	45.0	7
CGTAAGT	20	7.034623E-4	45.0	14
ATGCGAT	20	7.034623E-4	45.0	22
CCGAATA	45	3.8562575E-10	45.0	21
ATCGGCG	20	7.034623E-4	45.0	36
ACTACGT	30	2.1663636E-6	44.999996	43
GTCCGTT	30	2.1663636E-6	44.999996	9
ATACGTA	30	2.1663636E-6	44.999996	44
CGTTTTT	5745	0.0	42.76762	1
TCACGAC	780	0.0	42.692307	25
CGACGGT	765	0.0	42.64706	28
GTATGCG	235	0.0	42.12766	1
>>END_MODULE