FastQCFastQC Report
Thu 26 May 2016
SRR1545184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545184_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1143957
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35120.3070045464995625No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34810.3042946544319411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTC406.8157533E-945.00000443
TATTTCG207.033271E-445.00000412
ACCGGTA406.8157533E-945.00000441
GTCGACG207.033271E-445.0000041
ACGCATA207.033271E-445.00000414
CGATTGA406.8157533E-945.00000410
TCGATAT207.033271E-445.00000416
ATCGTCC406.8157533E-945.00000425
GTAATCG207.033271E-445.00000434
ATCCGAT406.8157533E-945.00000428
GTTACGT207.033271E-445.00000425
AATGCGG1550.045.0000042
GAACGAC207.033271E-445.00000418
ACGCTAG207.033271E-445.0000041
CGTCGTA207.033271E-445.00000430
GTATACG207.033271E-445.0000041
GTCCCGA207.033271E-445.00000443
CCGGTAT406.8157533E-945.00000442
ACCGTCC207.033271E-445.00000427
ACGTACT207.033271E-445.00000429