##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545184_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1143957 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16106636875337 31.0 31.0 33.0 30.0 34.0 2 31.446838473823753 31.0 31.0 34.0 30.0 34.0 3 31.55004515029848 31.0 31.0 34.0 30.0 34.0 4 35.30807102015198 37.0 35.0 37.0 33.0 37.0 5 35.16921090565467 37.0 35.0 37.0 32.0 37.0 6 35.2251631835812 37.0 35.0 37.0 32.0 37.0 7 35.79969264579001 37.0 35.0 37.0 35.0 37.0 8 35.81550355476648 37.0 35.0 37.0 35.0 37.0 9 37.560900453426136 39.0 37.0 39.0 35.0 39.0 10 36.75087699974737 39.0 37.0 39.0 32.0 39.0 11 36.35049481754996 38.0 35.0 39.0 32.0 39.0 12 35.310612199584426 37.0 35.0 39.0 30.0 39.0 13 34.78712224323117 37.0 33.0 39.0 27.0 39.0 14 35.68515949463136 37.0 34.0 40.0 27.0 41.0 15 36.121437256819966 37.0 34.0 40.0 30.0 41.0 16 36.34380575493659 37.0 35.0 40.0 31.0 41.0 17 36.2992551293449 37.0 35.0 40.0 31.0 41.0 18 36.24511585662748 37.0 35.0 40.0 31.0 41.0 19 36.1658751159353 37.0 35.0 40.0 31.0 41.0 20 35.9921220815118 36.0 34.0 40.0 30.0 41.0 21 35.825108810908105 36.0 34.0 40.0 30.0 41.0 22 35.726196876281186 36.0 34.0 40.0 30.0 41.0 23 35.70009362240014 36.0 34.0 40.0 30.0 41.0 24 35.66824539733574 36.0 34.0 40.0 30.0 41.0 25 35.59931448472277 36.0 34.0 40.0 30.0 41.0 26 35.40855731465431 35.0 34.0 40.0 29.0 41.0 27 35.32782438500748 35.0 34.0 40.0 29.0 41.0 28 35.38372421341012 36.0 34.0 40.0 29.0 41.0 29 35.409278495607786 36.0 34.0 40.0 29.0 41.0 30 35.3884621537348 36.0 34.0 40.0 29.0 41.0 31 35.15672092569913 36.0 34.0 40.0 29.0 41.0 32 34.988170884045466 35.0 34.0 40.0 29.0 41.0 33 34.79175703282554 35.0 33.0 40.0 27.0 41.0 34 34.67788299735042 35.0 34.0 40.0 27.0 41.0 35 34.4980248383462 35.0 33.0 40.0 26.0 41.0 36 34.288176041581984 35.0 33.0 40.0 24.0 41.0 37 34.19758522392013 35.0 33.0 40.0 24.0 41.0 38 34.21741201810907 35.0 33.0 40.0 25.0 41.0 39 34.21566981975721 35.0 33.0 40.0 24.0 41.0 40 34.03761155358112 35.0 33.0 40.0 23.0 41.0 41 34.05052987131509 35.0 33.0 40.0 24.0 41.0 42 33.97986725025503 35.0 33.0 39.0 24.0 41.0 43 33.90618703325387 35.0 33.0 39.0 24.0 41.0 44 33.82499254779681 35.0 33.0 39.0 23.0 41.0 45 33.757254861852324 35.0 33.0 39.0 23.0 41.0 46 33.673033164708116 35.0 33.0 39.0 23.0 41.0 47 33.59057377156659 35.0 33.0 39.0 23.0 41.0 48 33.6132450782678 35.0 33.0 39.0 23.0 41.0 49 33.61254575128261 35.0 33.0 39.0 24.0 41.0 50 33.48390367819769 35.0 32.0 39.0 24.0 41.0 51 33.33628099657592 35.0 32.0 38.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 2.0 13 2.0 14 8.0 15 27.0 16 92.0 17 253.0 18 645.0 19 1220.0 20 2232.0 21 3693.0 22 5679.0 23 8541.0 24 11657.0 25 15700.0 26 19995.0 27 23341.0 28 26323.0 29 30578.0 30 36889.0 31 45295.0 32 56182.0 33 73045.0 34 122518.0 35 179685.0 36 78094.0 37 94213.0 38 125932.0 39 181987.0 40 128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.78222608017609 24.382647249852923 30.188896960287842 13.646229709683144 2 29.417888959112975 25.994683366595073 31.364815285889243 13.22261238840271 3 27.01596301259575 26.285778224181506 32.89896385965557 13.79929490356718 4 25.253833841656636 27.643696397679285 33.09748530757712 14.00498445308696 5 22.798321964899028 32.92676210731697 30.43934343685995 13.835572490924047 6 20.48713369471055 40.99288697040186 27.184588231900324 11.335391102987263 7 88.54082802063364 4.181101212720408 5.845849100971453 1.4322216656744964 8 90.6927445699445 3.0929484237606832 4.9227374805171875 1.2915695257776298 9 87.25616434883479 4.305494000211547 5.992882599608202 2.4454590513454613 10 54.20081349211553 25.137483314495213 10.733095736990114 9.928607456399147 11 48.183279616279286 18.593093971189475 19.45099335027453 13.772633062256713 12 43.29559590089488 19.55982611234513 23.23557616239072 13.909001824369271 13 22.23868554499863 41.412133498024836 23.35306309590308 12.99611786107345 14 14.33987466312108 43.7199125491605 27.562924130889538 14.377288656828885 15 12.294780310798394 21.825033633257192 50.08055372710688 15.799632328837534 16 14.57860741269121 17.084645664128985 47.00299049702043 21.333756426159376 17 16.296241904197448 17.042686045017426 27.994933375992282 38.66613867479284 18 20.995194749452995 21.86970314443637 35.21137595206813 21.9237261540425 19 29.623228845140158 23.034956733513585 24.68449425983669 22.657320161509567 20 32.59851550364218 21.53236528995408 24.360705865692502 21.508413340711233 21 22.19226771635647 28.3248408812569 27.160374035038032 22.3225173673486 22 23.238023806838893 23.451755616688388 24.226959579774416 29.0832609966983 23 18.70280089199157 30.492492287734592 23.940148099972287 26.864558720301552 24 19.84567601754262 22.50556620572277 40.06610388327533 17.582653893459284 25 16.606306006257228 24.62006876132582 36.58432965574755 22.1892955766694 26 16.89713861622421 34.56222567806307 26.577047913514228 21.96358779219848 27 17.111657168932048 33.73186229901998 28.136809338113235 21.019671193934737 28 15.524184912544788 27.519216194314993 38.47723297291769 18.479365920222527 29 14.75317691136992 23.9768627666949 37.74486278767471 23.525097534260468 30 18.768712460345974 30.898976097877807 30.271155296921126 20.061156144855094 31 30.259441569919147 26.46183379270375 23.83367556647671 19.445049070900392 32 32.650615364039034 25.395185308538693 24.31927074181984 17.63492858560243 33 29.447960019476255 27.36606358455781 22.66737298692171 20.51860340904422 34 20.27593694518238 27.293858073336676 26.013302947575827 26.41690203390512 35 20.997205314535424 25.642397397804288 30.79713660565913 22.56326068200116 36 32.82090148493344 24.517005446883054 24.158862614591285 18.503230453592224 37 21.183226292596665 33.51681925107325 26.42616811645892 18.873786339871167 38 21.170463575116898 33.71927441328651 22.37155767218523 22.73870433941136 39 20.890995028659294 32.82334912938161 25.182852152659585 21.10280368929951 40 25.500608851556482 25.363103683092987 24.094786779573006 25.041500685777525 41 17.96684665594948 24.0053603413415 25.43959257209843 32.58820043061059 42 21.544341264575504 25.512934489670503 23.00497308902345 29.937751156730542 43 22.110096795596338 25.57263953103132 24.936339390379185 27.380924282993153 44 20.18458735774159 29.14908514917956 28.438131852858106 22.228195640220743 45 17.62557508717548 37.69564765109178 20.928933517605994 23.749843744126746 46 21.863758865062234 33.09748530757712 25.36065603864481 19.678099788715837 47 20.408022329510636 27.391589019517344 26.426780027570967 25.773608623401056 48 22.313426116541095 24.060607173171718 30.969258459889666 22.65670825039752 49 20.948689504937683 23.318096746643448 32.55244733849262 23.18076640992625 50 19.59190773779084 33.650041041752445 25.813382845683886 20.944668374772828 51 18.33093376761539 34.29822974115286 23.585589318479627 23.785247172752122 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 610.0 1 962.5 2 1315.0 3 3832.5 4 6350.0 5 4774.0 6 3198.0 7 3539.5 8 3881.0 9 4293.0 10 4705.0 11 4919.5 12 5134.0 13 5093.0 14 5052.0 15 4700.0 16 4348.0 17 4124.0 18 3900.0 19 4068.0 20 4236.0 21 3829.5 22 3423.0 23 3624.0 24 3825.0 25 4046.5 26 5800.0 27 7332.0 28 7723.0 29 8114.0 30 10043.5 31 11973.0 32 15502.5 33 19032.0 34 22532.0 35 26032.0 36 28907.0 37 31782.0 38 39169.5 39 46557.0 40 65168.5 41 83780.0 42 101308.0 43 118836.0 44 117984.5 45 117133.0 46 109764.0 47 102395.0 48 95286.5 49 88178.0 50 84082.5 51 79987.0 52 75246.0 53 70505.0 54 62442.0 55 54379.0 56 53023.5 57 51668.0 58 48078.5 59 44489.0 60 42966.0 61 41443.0 62 35453.5 63 29464.0 64 25075.5 65 20687.0 66 17097.0 67 13507.0 68 11029.5 69 8552.0 70 6950.5 71 5349.0 72 4702.5 73 4056.0 74 3042.0 75 1649.0 76 1270.0 77 837.5 78 405.0 79 352.5 80 300.0 81 334.0 82 368.0 83 212.0 84 56.0 85 36.5 86 17.0 87 26.5 88 36.0 89 19.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1143957.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.314684223263782 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61591489861775 13.152231949404545 2 7.334355190894868 2.3931537784141064 3 2.3678600488352024 1.1589266694488483 4 1.1421647476209829 0.7453622875352042 5 0.6996535015705988 0.5707312971912554 6 0.4816171238235035 0.4714458775018195 7 0.39921280825542665 0.4559121633198746 8 0.33934363031607256 0.4429027337426149 9 0.25944702587529334 0.3809516669838322 >10 3.327629543485535 13.087081409114637 >50 1.30015581635173 15.313483233685963 >100 1.7245868473095238 50.48517324516041 >500 0.006984308104368788 0.7296976567917824 >1k 0.0010745089391336596 0.6129460317050972 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 3512 0.3070045464995625 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3481 0.3042946544319411 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.014948114308492366 0.0 2 0.0 0.0 0.0 0.07780012710267956 0.0 3 0.0 0.0 0.0 0.12308154939390205 0.0 4 0.0 0.0 0.0 0.19415065426410258 0.0 5 0.0 0.0 0.0 0.3453801148120078 0.0 6 0.0 0.0 0.0 0.5050889150553736 0.0 7 0.0 0.0 0.0 0.6076277342592422 0.0 8 0.0 0.0 0.0 0.8640184902054885 0.0 9 0.0 0.0 0.0 0.995491963421702 0.0 10 0.0 0.0 0.0 1.2488231638077305 0.0 11 0.0 0.0 0.0 1.4192841164484329 0.0 12 0.0 0.0 0.0 1.5872974246409612 0.0 13 0.0 0.0 0.0 1.6480514564795705 0.0 14 0.0 0.0 0.0 1.6682445231770076 0.0 15 0.0 0.0 0.0 1.7062704279968566 0.0 16 0.0 0.0 0.0 1.7870426947866047 0.0 17 0.0 0.0 0.0 1.8864345425571065 0.0 18 0.0 0.0 0.0 2.044657272956938 0.0 19 0.0 0.0 0.0 2.10611063178074 0.0 20 0.0 0.0 0.0 2.170885793784207 0.0 21 0.0 0.0 0.0 2.2461508605655633 0.0 22 0.0 0.0 0.0 2.3171325495626145 0.0 23 8.741587314907816E-5 0.0 0.0 2.404985502077438 0.0 24 8.741587314907816E-5 0.0 0.0 2.4646905434382584 0.0 25 8.741587314907816E-5 0.0 0.0 2.51294410541655 0.0 26 3.4966349259631265E-4 0.0 0.0 2.5662677880374876 0.0 27 3.4966349259631265E-4 0.0 0.0 2.625186086539966 0.0 28 3.4966349259631265E-4 0.0 0.0 2.681918988213718 0.0 29 3.4966349259631265E-4 0.0 0.0 2.7420611089402835 0.0 30 3.4966349259631265E-4 0.0 0.0 2.8306133884403 0.0 31 3.4966349259631265E-4 0.0 0.0 2.901595077437351 0.0 32 3.4966349259631265E-4 0.0 0.0 2.960863039432426 0.0 33 3.4966349259631265E-4 0.0 0.0 3.025638201435893 0.0 34 3.4966349259631265E-4 0.0 0.0 3.0990675348811187 0.0 35 3.4966349259631265E-4 0.0 0.0 3.1981097191590244 0.0 36 3.4966349259631265E-4 0.0 0.0 3.272850290701486 0.0 37 3.4966349259631265E-4 0.0 0.0 3.3486398527217367 0.0 38 3.4966349259631265E-4 0.0 0.0 3.424779078234584 0.0 39 3.4966349259631265E-4 0.0 0.0 3.5037156116882016 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCGTC 40 6.8157533E-9 45.000004 43 TATTTCG 20 7.033271E-4 45.000004 12 ACCGGTA 40 6.8157533E-9 45.000004 41 GTCGACG 20 7.033271E-4 45.000004 1 ACGCATA 20 7.033271E-4 45.000004 14 CGATTGA 40 6.8157533E-9 45.000004 10 TCGATAT 20 7.033271E-4 45.000004 16 ATCGTCC 40 6.8157533E-9 45.000004 25 GTAATCG 20 7.033271E-4 45.000004 34 ATCCGAT 40 6.8157533E-9 45.000004 28 GTTACGT 20 7.033271E-4 45.000004 25 AATGCGG 155 0.0 45.000004 2 GAACGAC 20 7.033271E-4 45.000004 18 ACGCTAG 20 7.033271E-4 45.000004 1 CGTCGTA 20 7.033271E-4 45.000004 30 GTATACG 20 7.033271E-4 45.000004 1 GTCCCGA 20 7.033271E-4 45.000004 43 CCGGTAT 40 6.8157533E-9 45.000004 42 ACCGTCC 20 7.033271E-4 45.000004 27 ACGTACT 20 7.033271E-4 45.000004 29 >>END_MODULE